AB53007

Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y]

Name: Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) | Abcam
Brand: Abcam Limited
SKU: AB53007
Price: 390 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal ATP citrate lyase phospho S451 + T447 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, Citrate cleavage enzyme, ACL, ACLY

3 Images

Lab

Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (AB53007)

Blocking/Diluting Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 15 minutes whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 120 kDa

Observed band size: 122 kDa

false

Lab

Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (AB53007)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) treated with insulin whole cell lysate at 20 µg

Lane 2:

Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

Predicted band size: 120 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (AB53007)

Blocking/Diluting Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) treated with 100ng Calyculin A for 60 minutes whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 120 kDa

Observed band size: 122 kDa

false

Carrier free

Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP737Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab53007 only detects ATP-citrate lyase phosphorylated on Threonine 447 and Serine 451.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP citrate lyase (ACL ACLY) is an enzyme responsible for converting citrate and coenzyme A into acetyl-CoA and oxaloacetate using ATP in the process. This reaction is key for lipid and cholesterol biosynthesis. ACLY's alternate name is ATP citrate (pro-S)-lyase and it has a molecular weight of approximately 120 kDa. The enzyme is expressed mainly in the cytoplasm with substantial amounts found in liver and adipose tissues.

Biological function summary

ATP citrate lyase plays an important role in lipid metabolism and energy production. It drives the conversion of citrate-derived acetyl-CoA a central metabolite in lipogenesis providing substrates for fatty acid and cholesterol synthesis. ACLY functions as a homotetramer complex to facilitate its enzymatic activity. This mechanism supports energy homeostasis linking carbohydrate metabolism with lipid biosynthesis.

Pathways

The enzyme ATP citrate lyase is instrumental in the citric acid cycle and fatty acid synthesis pathways. It is a central player in the cholesterol biosynthesis pathway which tightly connects to acetyl-CoA and citrate shuttle processes. In these pathways it interacts functionally with other enzymes such as acetyl-CoA carboxylase which further processes acetyl-CoA into malonyl-CoA serving as a precursor for fatty acid elongation.

ATP citrate lyase becomes significant in metabolic syndrome and cancer. Elevated expression and activity are linked to an increased risk of metabolic diseases including obesity and type 2 diabetes due to its role in excessive lipid accumulation. Moreover cancer cells often exhibit upregulated ACLY activity encouraging tumor growth by supplying acetyl-CoA for lipid biosynthesis. Its activity is intricately linked to proteins such as fatty acid synthase which also contribute to altered lipid profiles in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate in multiple biochemical reactions in protein, carbohydrate and lipid metabolism.

See full target information ATP-citrate synthase phospho S451 + T447

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 9: PubMed31614639

2019

Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando E García-Arroyo,Fabiola Monroy-Sánchez,Itzel Muñoz-Jiménez,Guillermo Gonzaga,Ana Andrés-Hernando,Cecilia Zazueta,J Gabriel Juárez-Rojas,Miguel A Lanaspa,Richard J Johnson,L Gabriela Sánchez-Lozada

Journal of experimental & clinical cancer research 37:324 PubMed30591064

2018

Hepatocellular carcinoma-derived exosomal miRNA-21 contributes to tumor progression by converting hepatocyte stellate cells to cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhou,Haozhen Ren,Bo Dai,Jun Li,Longcheng Shang,Jianfei Huang,Xiaolei Shi

Journal of proteomics 74:2866-80 PubMed21787891

2011

A comparative proteome analysis links tyrosine kinase 2 (Tyk2) to the regulation of cellular glucose and lipid metabolism in response to poly(I:C).

Applications

Species

Unspecified reactive species

Tom Grunert,Nicole R Leitner,Martina Marchetti-Deschmann,Ingrid Miller,Barbara Wallner,Marta Radwan,Claus Vogl,Thomas Kolbe,Dagmar Kratky,Manfred Gemeiner,Günter Allmaier,Mathias Müller,Birgit Strobl

The Journal of biological chemistry 285:32606-15 PubMed20693577

2010

ATP-citrate lyase reduction mediates palmitate-induced apoptosis in pancreatic beta cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kwan Yi Chu,Yalin Lin,Alon Hendel,Jerzy E Kulpa,Roger W Brownsey,James D Johnson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y]

Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (AB53007)

Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (AB53007)

Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (AB53007)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise