AB181243

Anti-ATP synthase C antibody [EPR13907]

RabMAb
Recombinant
20ul selling size
What is this?

Be the first to review this product! Submit a review

(58 Publications)

Anti-ATP synthase C antibody [EPR13907] (ab181243) is a rabbit monoclonal antibody detecting ATP synthase C in Western Blot, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications

View Alternative Names

ATP5G1, ATP5MC1, ATP synthase lipid-binding protein, ATP synthase membrane subunit c locus 1, ATP synthase proteolipid P1, ATP synthase proton-transporting mitochondrial F(0) complex subunit C1, ATPase protein 9, ATPase subunit c

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATP synthase C antibody [EPR13907] (AB181243)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATP synthase C antibody [EPR13907] (AB181243)

Immunofluorescent analysis of acetone-fixed HL-60 cells labeling ATP synthase C with ab181243 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

Supplier Data

Western blot - Anti-ATP synthase C antibody [EPR13907] (AB181243)

All lanes:

Western blot - Anti-ATP synthase C antibody [EPR13907] (ab181243) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human fetal heart lysate at 20 µg

Lane 3:

HL-60 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 8 kDa

false

Carrier free

Anti-ATP synthase C antibody [EPR13907] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ATP synthase C antibody [EPR13907]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-ATP synthase C antibody [EPR13907]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13907

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-ATP synthase C antibody [EPR13907] (ab181243) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of ATP synthase C?
Anti-ATP synthase C [EPR13907] (ab181243) specifically detects a band for ATP synthase C (UniProt: P05496) at a molecular weight of 14kDa.

Trusted by the scientific community
Anti-ATP synthase C [EPR13907] (ab181243) was first used in a scientific publication in 2014 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR13907] also available for your convenience: ab181243, Alexa Fluor® 488 - ab210456, Alexa Fluor® 555 - ab210732, Carrier free - ab232644

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP synthase subunit c also known as ATP synthase C ATP-C or ATP synthase C subunit is an integral component of the ATP synthase complex. This protein has a known molecular mass of approximately 8.3 kDa. It is prominently expressed in mitochondrial membranes where it serves as an important part of the proton channel. Its primary function involves facilitating the conversion of proton motive force into rotational mechanical energy which drives ATP synthesis. Through this mechanical action ATP synthase C contributes significantly to energy conversion within cells.

Biological function summary

ATP synthase C functions as a critical component of the ATP synthase enzyme complex also known as FoF1 ATPase or Complex V of the oxidative phosphorylation pathway. This subunit forms a ring structure that allows protons to move across the mitochondrial membrane which is necessary for the synthesis of ATP from ADP and inorganic phosphate. By participating in this synthesis process ATP synthase C impacts cellular energy metabolism affecting various physiological functions dependent on ATP as an energy source.

Pathways

ATP synthase C plays an essential role within the oxidative phosphorylation pathway. This pathway is integral to cellular respiration and is responsible for the majority of ATP production in aerobic organisms. ATP synthase C interacts with other proteins such as the ATP synthase alpha and beta subunits which form the catalytic core of the enzyme. These interactions are central to the energy currency of the cell ensuring efficient energy conversion and transfer.

ATP synthase C is implicated in mitochondrial dysfunctions and associated conditions such as mitochondrial myopathies and neurodegenerative diseases. Mitochondrial myopathies result from defects in the oxidative phosphorylation pathway often involving proteins like cytochrome c oxidase which are important for efficient ATP production. Disorders related to ATP synthase C dysfunction can cause significant impacts on tissues that require high energy reflecting its critical role in maintaining cellular energy balance.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Subunit c, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). With the subunit a (MT-ATP6), forms the proton-conducting channel in the F(0) domain, that contains two crucial half-channels (inlet and outlet) that facilitate proton movement from the mitochondrial intermembrane space (IMS) into the matrix (PubMed : 37244256). Protons are taken up via the inlet half-channel and released through the outlet half-channel, following a Grotthuss mechanism (PubMed : 37244256).

See full target information ATP5MC1

style

left-column

Publications (58)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:26217 PubMed40683940

2025

TFEB overexpression alleviates autophagy-lysosomal deficits caused by progranulin insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Wren O Nader,Kaylan S Brown,Nicholas R Boyle,Azariah K Kaplelach,Shaimaa M Abdelaziz,Skylar E Davis,Qays Aljabi,Ahmad R Hakim,Amelia G Davidson,Giacynta A Vollmer,Leah C Wright,J Bailey Echols,Joelle Saad,Nicholas S Pena,Andrew E Arrant

Molecular psychiatry 30:4591-4604 PubMed40346285

2025

CLN5 deficiency impairs glucose uptake and uncovers PHGDH as a potential biomarker in Batten disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Marchese,Sara Bernardi,Rachele Vivarelli,Stefano Doccini,Lorenzo Santucci,Asahi Ogi,Rosario Licitra,Jingjing Zang,Rabah Soliymani,Serena Mero,Stephan Cf Neuhauss,Lea Ciarmoli,Giovanni Signore,Maciej M Lalowski,Filippo M Santorelli

Nature communications 16:4029 PubMed40301431

2025

Mitochondrial membrane hyperpolarization modulates nuclear DNA methylation and gene expression through phospholipid remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Mateus Prates Mori,Oswaldo A Lozoya,Ashley M Brooks,Carl D Bortner,Cristina A Nadalutti,Birgitta Ryback,Brittany P Rickard,Marta Overchuk,Imran Rizvi,Tatiana Rogasevskaia,Kai Ting Huang,Prottoy Hasan,György Hajnóczky,Janine H Santos

Aging and disease 16:3040-3054 PubMed39571159

2024

Exercise Types: Physical Activity Mitigates Cardiac Aging and Enhances Mitochondrial Function via PKG-STAT3-Opa1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Reka Szekeres,Daniel Priksz,Mariann Bombicz,Beata Pelles-Tasko,Anna Szilagyi,Brigitta Bernat,Aniko Posa,Balazs Varga,Rudolf Gesztelyi,Sandor Somodi,Zoltan Szabo,Zoltan Szilvassy,Bela Juhasz

Communications biology 7:1373 PubMed39438652

2024

Loss of CLN3 in microglia leads to impaired lipid metabolism and myelin turnover.

Applications

Unspecified application

Species

Unspecified reactive species

Seda Yasa,Elisabeth S Butz,Alessio Colombo,Uma Chandrachud,Luca Montore,Sarah Tschirner,Matthias Prestel,Steven D Sheridan,Stephan A Müller,Janos Groh,Stefan F Lichtenthaler,Sabina Tahirovic,Susan L Cotman

Scientific reports 14:17469 PubMed39080379

2024

TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

D Wünkhaus,R Tang,K Nyame,N N Laqtom,M Schweizer,A Scotto Rosato,E K Krogsæter,C Wollnik,M Abu-Remaileh,C Grimm,G Hermey,R Kuhn,D Gruber-Schoffnegger,S Markmann

Cells 13: PubMed38786099

2024

Heterologous HSPC Transplantation Rescues Neuroinflammation and Ameliorates Peripheral Manifestations in the Mouse Model of Lysosomal Transmembrane Enzyme Deficiency, MPS IIIC.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefang Pan,Antoine Caillon,Shuxian Fan,Shaukat Khan,Shunji Tomatsu,Alexey V Pshezhetsky

Neurobiology of disease 197:106536 PubMed38763444

2024

Targeting autophagy impairment improves the phenotype of a novel CLN8 zebrafish model.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Marchese,Sara Bernardi,Asahi Ogi,Rosario Licitra,Giada Silvi,Serena Mero,Daniele Galatolo,Nicola Gammaldi,Stefano Doccini,Gian Michele Ratto,Simona Rapposelli,Stephan C F Neuhauss,Jingjing Zang,Silvia Rocchiccioli,Elena Michelucci,Elisa Ceccherini,Filippo M Santorelli

Phytotherapy research : PTR 38:1990-2006 PubMed38372204

2024

Quercetin as a promising intervention for rat osteoarthritis by decreasing M1-polarized macrophages via blocking the TRPV1-mediated P2X7/NLRP3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Li,Hebei He,Min Du,Mu Gao,Qijie Sun,Yeyang Wang,Hanyu Lu,Shuanji Ou,Changliang Xia,Changpeng Xu,Qi Zhao,Hongtao Sun

PloS one 19:e0295737 PubMed38165883

2024

Direct toxicity of cigarette smoke extract on cardiac function mediated by mitochondrial dysfunction in Sprague-Dawley rat ventricular myocytes and human induced pluripotent stem cell-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sakiko Matsumura,Jumpei Yasuda,Takuya Notomi,Yoshihiro Suzuki,I-Shan Chen,Daichi Murakami,Muneki Hotomi,Tomoe Y Nakamura

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP synthase C antibody [EPR13907]

Immunocytochemistry/ Immunofluorescence - Anti-ATP synthase C antibody [EPR13907] (AB181243)

Western blot - Anti-ATP synthase C antibody [EPR13907] (AB181243)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (58)

Product promise