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AB109867

Anti-ATP synthase Immunocapture antibody [12F4AD8AF8]

4

(3 Reviews)

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(27 Publications)

Mouse Monoclonal ATPase Inhibitory Factor 1/IF1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 27 publications.

View Alternative Names

ATPI, ATPIF1, ATP5IF1, ATP synthase F1 subunit epsilon, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1

1 Images
Flow Cytometry - Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (AB109867)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (AB109867)

Overlay histogram showing HepG2 cells stained with ab109867 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109867, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

12F4AD8AF8

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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AB109714

ATP synthase Activity Assay Kit (Colorimetric)

Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714)

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Primary Antibodies

AB14730

Anti-ATPB antibody [3D5] - Mitochondrial Marker

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Primary Antibodies

AB181243

Anti-ATP synthase C antibody [EPR13907]

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Immunocytochemistry/ Immunofluorescence - Anti-ATP synthase C antibody [EPR13907] (AB181243)

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Assay Kits

AB109715

ATP Synthase Immunocapture Kit

Western blot - ATP Synthase Immunocapture Kit (AB109715)

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Reagents, Controls & Accessories

AB110411

Total OXPHOS Human WB Antibody Cocktail

Western blot - Total OXPHOS Human WB Antibody Cocktail (AB110411)

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  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab109867 is a sample of pure immunocapture antibody, not immobilized to a solid support.

This antibody was tested and confirmed not suitable for WB and ICC.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification notes
The purity of ab109867 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP synthase also known as Complex V functions mechanically as a pivotal component in cellular energy production. It is a complex enzyme with a mass of approximately 600 kDa composed of multiple subunits. ATP synthase localizes primarily to the inner membrane of mitochondria though it is also found in chloroplasts and bacteria. The enzyme facilitates the synthesis of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate driven by a proton gradient established across the membrane.
Biological function summary

ATP synthase plays an essential role in cellular metabolism by converting energy stored in the proton gradient into chemical energy in the form of ATP the universal energy currency. It functions as part of the mitochondrial respiratory chain complex working alongside other complexes such as NADH dehydrogenase and cytochrome c oxidase. ATP synthase is important for energy-intensive processes as it ensures cells have adequate ATP to maintain homeostasis and perform vital functions.

Pathways

The function of ATP synthase links closely to oxidative phosphorylation and photosynthesis two important biological pathways. In oxidative phosphorylation ATP synthase acts in concert with complexes I-IV of the electron transport chain utilizing the proton gradient generated by electron transfers. The enzyme also interacts with proteins like cytochrome c and ATP/ADP translocase which facilitate electron transfer and ATP exchange respectively. These interactions highlight ATP synthase's integral role in energy production pathways.

Disruptions in ATP synthase function can lead to metabolic disorders such as mitochondrial encephalomyopathy and Leigh syndrome. These conditions involve mutations that impair ATP production and disrupt energy balance in cells. In these disorders ATP synthase remains linked to proteins implicated in mitochondrial function including cytochrome c oxidase and succinate dehydrogenase. These connections emphasize the enzyme's importance in maintaining mitochondrial integrity and energy homeostasis within cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix (PubMed : 35926043). Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues : regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
See full target information ATP5IF1
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Gels (Basel, Switzerland) 11: PubMed40710654

2025

Pushing Optical Resolution to the Few-Nanometer Scale via dSTORM Imaging of Expanded Specimen-Gel Composites.

Applications

Unspecified application

Species

Unspecified reactive species

Jimmy Ching-Cheng Hsu,T Tony Yang

ACS nano 18:23445-23456 PubMed39143924

2024

Multiplexed Nanoscopy via Buffer Exchange.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Jui Ben Chang,T Tony Yang

Communications biology 6:427 PubMed37072500

2023

Spatial and temporal dynamics of ATP synthase from mitochondria toward the cell surface.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Wen Chang,T Tony Yang,Min-Chun Chen,Y-Geh Liaw,Chieh-Fan Yin,Xiu-Qi Lin-Yan,Ting-Yu Huang,Jen-Tzu Hou,Yi-Hsuan Hung,Chia-Lang Hsu,Hsuan-Cheng Huang,Hsueh-Fen Juan

Nature communications 14:1688 PubMed36973278

2023

Single-molecule localization microscopy reveals the ultrastructural constitution of distal appendages in expanded mammalian centrioles.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Jui Ben Chang,Jimmy Ching-Cheng Hsu,T Tony Yang

Molecular & cellular proteomics : MCP 21:100237 PubMed35439648

2022

Quantitative phosphoproteomics reveals ectopic ATP synthase on mesenchymal stem cells to promote tumor progression via ERK/c-Fos pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Wen Chang,Chia-Chi Wang,Chieh-Fan Yin,Chang-Hsun Wu,Hsuan-Cheng Huang,Hsueh-Fen Juan

iScience 25:103605 PubMed35005550

2022

Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Roseth Aass,Robin Mjelle,Martin H Kastnes,Synne S Tryggestad,Luca M van den Brink,Ingrid Aass Roseth,Marita Westhrin,Muhammad Zahoor,Siv H Moen,Tonje M Vikene Nedal,Glenn Buene,Kristine Misund,Anne-Marit Sponaas,Qianli Ma,Anders Sundan,Richard Wj Groen,Tobias S Slørdahl,Anders Waage,Therese Standal

Biochimica et biophysica acta. Molecular cell research 1868:118942 PubMed33359711

2020

TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel.

Applications

Unspecified application

Species

Unspecified reactive species

Hela Bahri,Jeremie Buratto,Manuel Rojo,Jim Paul Dompierre,Bénédicte Salin,Corinne Blancard,Sylvain Cuvellier,Marie Rose,Amel Ben Ammar Elgaaied,Emmanuel Tetaud,Jean-Paul di Rago,Anne Devin,Stéphane Duvezin-Caubet

Nutritional neuroscience :1-11 PubMed33023416

2020

Hydroxytyrosol improves mitochondrial energetics of a cellular model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Visioli,María Rodríguez-Pérez,Óscar Gómez-Torres,Cristina Pintado-Losa,Emma Burgos-Ramos

Cell 183:76-93.e22 PubMed32931733

2020

Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyi Zhao,Jiayu Liu,Hong Deng,Ruiying Ma,Jian-You Liao,Huixin Liang,Jingxiong Hu,Jiaqian Li,Zhiyong Guo,Junchao Cai,Xiaoding Xu,Zhiliang Gao,Shicheng Su

Molecular & cellular proteomics : MCP 19:1805-1825 PubMed32788343

2020

Multiomics Reveals Ectopic ATP Synthase Blockade Induces Cancer Cell Death via a lncRNA-mediated Phospho-signaling Network.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Wen Chang,Chia-Lang Hsu,Cheng-Wei Tang,Xiang-Jun Chen,Hsuan-Cheng Huang,Hsueh-Fen Juan
View all publications
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Product promise

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