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Mouse Monoclonal ATPase Inhibitory Factor 1/IF1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 22 publications.


Images

Flow Cytometry - Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (AB109867), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow CytWB
Human
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.

Alternative names

ATPI, ATPIF1, ATP5IF1, ATP synthase F1 subunit epsilon, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1

Recommended products

Mouse Monoclonal ATPase Inhibitory Factor 1/IF1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 22 publications.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
12F4AD8AF8
Light chain type
kappa
Concentration
Loading...
Purification notes

The purity of ab109867 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab109867 is a sample of pure immunocapture antibody, not immobilized to a solid support.

This antibody was tested and confirmed not suitable for WB and ICC.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATP synthase also known as Complex V functions mechanically as a pivotal component in cellular energy production. It is a complex enzyme with a mass of approximately 600 kDa composed of multiple subunits. ATP synthase localizes primarily to the inner membrane of mitochondria though it is also found in chloroplasts and bacteria. The enzyme facilitates the synthesis of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate driven by a proton gradient established across the membrane.

Biological function summary

ATP synthase plays an essential role in cellular metabolism by converting energy stored in the proton gradient into chemical energy in the form of ATP the universal energy currency. It functions as part of the mitochondrial respiratory chain complex working alongside other complexes such as NADH dehydrogenase and cytochrome c oxidase. ATP synthase is important for energy-intensive processes as it ensures cells have adequate ATP to maintain homeostasis and perform vital functions.

Pathways

The function of ATP synthase links closely to oxidative phosphorylation and photosynthesis two important biological pathways. In oxidative phosphorylation ATP synthase acts in concert with complexes I-IV of the electron transport chain utilizing the proton gradient generated by electron transfers. The enzyme also interacts with proteins like cytochrome c and ATP/ADP translocase which facilitate electron transfer and ATP exchange respectively. These interactions highlight ATP synthase's integral role in energy production pathways.

Associated diseases and disorders

Disruptions in ATP synthase function can lead to metabolic disorders such as mitochondrial encephalomyopathy and Leigh syndrome. These conditions involve mutations that impair ATP production and disrupt energy balance in cells. In these disorders ATP synthase remains linked to proteins implicated in mitochondrial function including cytochrome c oxidase and succinate dehydrogenase. These connections emphasize the enzyme's importance in maintaining mitochondrial integrity and energy homeostasis within cells.

Product promise

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1 product image

  • Flow Cytometry - Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867), expandable thumbnail

    Flow Cytometry - Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] (ab109867)

    Overlay histogram showing HepG2 cells stained with ab109867 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109867, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Product protocols

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