JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB105351

Anti-ATP11A antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal ATP11A antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATP11A.

View Alternative Names

ATPIH, ATPIS, KIAA1021, ATP11A, Phospholipid-transporting ATPase IH, ATPase IS, ATPase class VI type 11A, P4-ATPase flippase complex alpha subunit ATP11A

3 Images
Immunocytochemistry - Anti-ATP11A antibody (AB105351)
  • ICC

Unknown

Immunocytochemistry - Anti-ATP11A antibody (AB105351)

ab105351, at 10 μg/ml, staining ATP11A in K562 cells by Immunocytochemistry.

Immunocytochemistry - Anti-ATP11A antibody (AB105351)
  • ICC

Unknown

Immunocytochemistry - Anti-ATP11A antibody (AB105351)

Immunofluorescence of ATP11A in K562 cells with ab105351 at 20 ug/mL.

Western blot - Anti-ATP11A antibody (AB105351)
  • WB

Unknown

Western blot - Anti-ATP11A antibody (AB105351)

All lanes:

Western blot - Anti-ATP11A antibody (ab105351) at 1 µg/mL

Lane 1:

K562 cell lysate at 15 µg

Lane 2:

K562 cell lysate at 15 µg with blocking peptide

Predicted band size: 129 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human ATP11A. The exact immunogen used to generate this antibody is proprietary information.

P98196

Specificity

ab105351 is predicted to not cross react with AT11B. At least two isoforms of ATP11A are known to exist; this antibody will recognize both.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10-20 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP11A also known as AtpIH is a phospholipid-transporting ATPase located on the plasma membrane important for maintaining phospholipid asymmetry. Its approximate molecular weight is 130 kDa. This enzyme primarily expresses in tissues such as the brain testis and heart highlighting its important functions in these areas. ATP11A works in the flipping of aminophospholipids from the outer to the inner leaflet of cellular membranes playing an important role in maintaining membrane stability and function.
Biological function summary

ATP11A and its activity contribute to cellular processes including vesicle formation and transport across the membrane. It often functions as a part of a larger protein complex which facilitates its phospholipid translocating action. By coordinating with specific binding partners ATP11A supports cellular morphology and communication ultimately assisting in developmental and functional processes in several tissues.

Pathways

ATP11A participates significantly in the phospholipid transport pathway and cellular homeostasis. It closely interacts with CDC50 proteins such as CDC50A which act as regulatory subunits assisting in the flipping activity. On a broader scale ATP11A influences membrane trafficking pathways and the asymmetry of phospholipids linking it to key cellular events like apoptosis and immune response. These interactions enhance the precision and efficiency of its role in cellular function.

Disrupted ATP11A function associates with immune disorders and neurological conditions. Aberrations in its normal activity can lead to immune system dysfunctions and specific types of neuropathies. The dysregulation of ATP11A also links it to Alzheimer's disease where improper membrane phospholipid distribution might influence neurodegenerative processes. Understanding the interaction between ATP11A and related proteins like APP (amyloid precursor protein) aids in clarifying its role in these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids, phosphatidylserines (PS) and phosphatidylethanolamines (PE), from the outer to the inner leaflet of the plasma membrane (PubMed : 25315773, PubMed : 25947375, PubMed : 26567335, PubMed : 29799007, PubMed : 30018401, PubMed : 36300302). Does not show flippase activity toward phosphatidylcholine (PC) (PubMed : 34403372). Contributes to the maintenance of membrane lipid asymmetry with a specific role in morphogenesis of muscle cells. In myoblasts, mediates PS enrichment at the inner leaflet of plasma membrane, triggering PIEZO1-dependent Ca2+ influx and Rho GTPases signal transduction, subsequently leading to the assembly of cortical actomyosin fibers and myotube formation (PubMed : 29799007). May be involved in the uptake of farnesyltransferase inhibitor drugs, such as lonafarnib.
See full target information ATP11A
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 22:1775-84 PubMed25822340

2015

Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry.

Applications

Unspecified application

Species

Unspecified reactive species

S Marathe,S Liu,E Brai,M Kaczarowski,L Alberi
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB105377

Anti-ATP11B antibody

Immunocytochemistry - Anti-ATP11B antibody (AB105377)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB76286

Anti-beta III Tubulin (phospho S172) antibody

Western blot - Anti-beta III Tubulin (phospho S172) antibody (AB76286)

  • 4
  • 1
Primary Antibodies

AB288304

Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NOL3 antibody [EPR25182-11] - BSA and Azide free (AB288304)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com