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AB105377

Anti-ATP11B antibody

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(1 Publication)

Rabbit Polyclonal ATP11B antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATP11B.

View Alternative Names

ATPIF, ATPIR, KIAA0956, ATP11B, Phospholipid-transporting ATPase IF, ATPase IR, ATPase class VI type 11B, P4-ATPase flippase complex alpha subunit ATP11B

3 Images
Immunocytochemistry - Anti-ATP11B antibody (AB105377)
  • ICC

Unknown

Immunocytochemistry - Anti-ATP11B antibody (AB105377)

Immunofluorescence of ATP11B in K562 cells with ab105377 at 20 ug/mL.

Immunocytochemistry - Anti-ATP11B antibody (AB105377)
  • ICC

Unknown

Immunocytochemistry - Anti-ATP11B antibody (AB105377)

ab105377 at 10 µg/ml staining ATP11B in K562 cells by Immunocytochemistry

Western blot - Anti-ATP11B antibody (AB105377)
  • WB

Unknown

Western blot - Anti-ATP11B antibody (AB105377)

All lanes:

Western blot - Anti-ATP11B antibody (ab105377) at 1 µg/mL

Lane 1:

K562 cell tissue lysate without blocking peptide at 15 µg

Lane 2:

K562 cell tissue lysate with blocking peptide at 15 µg

Predicted band size: 134 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human ATP11B. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2G3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10-20 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP11B also known as a P-type ATPase acts as a phospholipid transporter that assists in maintaining cellular membrane organization by flipping phospholipids between the inner and outer layers of cell membranes. It has an approximate molecular mass of 150 kDa. This protein is predominantly expressed in the human prostate liver and colorectal tissues. Researchers recognize ATP11B's role in various cellular processes due to its transport activity.
Biological function summary

The ATP11B protein is involved in maintaining the proper distribution of phospholipids across different cellular membranes which is essential for cell membrane stability and function. It often functions as part of a larger protein complex particularly involving transmembrane proteins. This coordination facilitates the efficient movement of specific lipids a process vital for cellular functions like vesicle formation and endocytosis.

Pathways

ATP11B plays a significant role in the vesicular trafficking pathway and lipid signaling pathway. In these pathways ATP11B interacts with other proteins such as CDC50A which serves as an essential subunit for ATPase activity and function. These interactions enable the protein to mediate lipid movement and support various cellular processes including protein sorting and signal transduction.

Research associates ATP11B with cancer progression particularly ovarian cancer and drug resistance mechanisms. ATP11B expression correlates with the development of cisplatin resistance in ovarian cancer cells possibly by altering drug distribution within the cells. Moreover ATP11B might interact with other oncogenic proteins such as MRP2 (Multidrug Resistance Protein 2) contributing to chemotherapy resistance and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids, phosphatidylserines (PS) and phosphatidylethanolamines (PE), from the outer to the inner leaflet of intracellular membranes (PubMed : 30018401). May contribute to the maintenance of membrane lipid asymmetry in endosome compartment (PubMed : 30018401).
See full target information ATP11B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular vision 22:1387-1404 PubMed28003730

2016

Accumulation of cholesterol and increased demand for zinc in serum-deprived RPE cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sanghamitra Mishra,Katherine Peterson,Lili Yin,Alan Berger,Jianguo Fan,Graeme Wistow
View all publications
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Product promise

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