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AB275647

Anti-ATP12A antibody

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Rabbit Polyclonal ATP12A antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ATP12A.

View Alternative Names

ATP1AL1, ATP12A, Potassium-transporting ATPase alpha chain 2, HK alpha 2, Non-gastric H(+)/K(+) ATPase subunit alpha, Non-gastric Na(+)/K(+) ATPase subunit alpha, Proton pump, Sodium pump

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP12A antibody (AB275647)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP12A antibody (AB275647)

Immunochemical staining of human ATP12A in human kidney with ab275647 at 1 : 200 dilution, formalin-fixed paraffin embedded sections.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ATP12A. The exact immunogen used to generate this antibody is proprietary information.

P54707

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Preservative: 0.03% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ATP12A protein also known as gastric H+/K+ ATPase functions as a proton pump in cellular processes. It facilitates the exchange of potassium ions (K+) and hydrogen ions (H+) across cell membranes playing an essential role in ion homeostasis. The ATP12A protein has a molecular mass of approximately 116 kDa. Expression of ATP12A is found primarily in the gastric epithelium where it contributes to the acidification of the stomach although it can also be present in other tissues such as the colon.
Biological function summary

Ion transport is significantly influenced by ATP12A. This protein reforms a part of an enzyme complex that provides energy for H+ ion secretion. By coupling ATP hydrolysis to the transport process ATP12A catalyzes reactions vital for maintaining the acidic environment in the stomach. The protein's activity relates closely with potassium channels with which it might interact to optimize gastric acid secretion.

Pathways

ATP12A is integral to the gastric acid secretion pathway. Through this pathway the protein collaborates with the neighboring potassium chloride transporters to regulate acid release in the gastric lumen. ATP12A also shares a functional relationship with ATP1AL1 another ATPase variant in facilitating ATP-dependent ion transport. These pathways and interactions highlight the protein's central role in maintaining cellular processes related to pH balance.

ATP12A is linked with certain gastric pathologies such as peptic ulcers and gastritis. The protein's role in abnormal gastric acid secretion can exacerbate these conditions highlighting its impact on gastrointestinal health. Malfunction in related proteins such as ATP4A can further influence the pathological outcomes associated with ATP12A activity leading to exacerbated symptoms of gastric dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The catalytic subunit of a H(+)/K(+) ATPase and/or Na(+)/K(+) ATPase pump which transports K(+) ions in exchange for Na(+) and/or H(+) ions across the apical membrane of epithelial cells. Uses ATP as an energy source to pump K(+) ions into the cell while transporting Na(+) and/or H(+) ions to the extracellular compartment (PubMed : 11341842, PubMed : 7485470, PubMed : 8853415, PubMed : 9774385). Involved in the maintenance of electrolyte homeostasis through K(+) ion absorption in kidney and colon (By similarity). In the airway epithelium, may play a primary role in mucus acidification regulating its viscosity and clearance (PubMed : 29391451).
See full target information ATP12A
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