Skip to main content

Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture) (AB322686), expandable thumbnail
  • Sandwich ELISA - Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture) (AB322686), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested
Mouse
Tested

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Target data

Function

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (PubMed:17408578).

Alternative names

Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR29444-721

Purification technique

Affinity purification Protein A

Specificity

This antibody has 11% cross reaction with mouse ATP1A2, 100% cross reaction with mouse ATP1A3 and 10% cross reaction with mouse ATP1A4. The antibody may cross-react with human ATP1A3, but no cross-reactivity testing has been performed.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab322686 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse ATP1A1 + ATP1A3. The recommended pair for sandwich ELISA is:

Capture: ab322686, Human/Mouse ATP1A1 + ATP1A3 Capture Antibody (unconjugated)

Detector: Anti-ATP1A1 antibody [EPR29444-505] - BSA and Azide free (Detector) ab322687, Human/Mouse ATP1A1 Detector Antibody (unconjugated)

The reference range value is 31.25-2000 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture) (ab322686), expandable thumbnail

    Sandwich ELISA - Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture) (ab322686)

    Interpolated concentration of native alpha 1 Sodium Potassium ATPase was measured at different sample concentrations. Undiluted samples are as follows: Neuro-2a cell extract (25 ug/mL) and SH-SY5Y cell extract (200 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (Antibody Diluent CP2 ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture) (ab322686), expandable thumbnail

    Sandwich ELISA - Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture) (ab322686)

    Sandwich ELISA of Human/Mouse ATP1A1 Antibody Pair - BSA and Azide free ab322685 with the capture antibody ab322686 dilution at 2 ug/mL and detector antibody Anti-ATP1A1 antibody [EPR29444-505] - BSA and Azide free (Detector) ab322687 dilution at 0.5 ug/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com