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AB322686

Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1

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Sandwich ELISA - Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture) (AB322686)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture) (AB322686)

Sandwich ELISA of ab322685 with the capture antibody ab322686 dilution at 2 ug/mL and detector antibody ab322687 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture) (AB322686)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ATP1A1 + ATP1A3 antibody [EPR29444-721] - BSA and Azide free (Capture) (AB322686)

Interpolated concentration of native alpha 1 Sodium Potassium ATPase was measured at different sample concentrations. Undiluted samples are as follows : Neuro-2a cell extract (25 ug/mL) and SH-SY5Y cell extract (200 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29444-721

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has 11% cross reaction with mouse ATP1A2, 100% cross reaction with mouse ATP1A3 and 10% cross reaction with mouse ATP1A4. The antibody may cross-react with human ATP1A3, but no cross-reactivity testing has been performed.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab322686 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse ATP1A1 + ATP1A3. The recommended pair for sandwich ELISA is:

Capture: ab322686, Human/Mouse ATP1A1 + ATP1A3 Capture Antibody (unconjugated)

Detector: ab322687, Human/Mouse ATP1A1 Detector Antibody (unconjugated)

The reference range value is 31.25-2000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (PubMed : 17408578).
See full target information Atp1a1
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Complementary Products

Assay Kits

AB324503

Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular

SimpleStep

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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