AB166888

Anti-ATP1A2 antibody [EPR11896(B)]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal AT1A2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

KIAA0778, ATP1A2, Sodium/potassium-transporting ATPase subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, Sodium pump subunit alpha-2

2 Images

Supplier Data

Western blot - Anti-ATP1A2 antibody [EPR11896(B)] (AB166888)

All lanes:

Western blot - Anti-ATP1A2 antibody [EPR11896(B)] (ab166888) at 1/1000 dilution

Lane 1:

Human cerebellum lysate unboiled at 20 µg

Lane 2:

Human cerebellum lysate boiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 102 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-ATP1A2 antibody [EPR11896(B)] (AB166888)

All lanes:

Western blot - Anti-ATP1A2 antibody [EPR11896(B)] (ab166888) at 1/1000 dilution

Lane 1:

Mouse brain lysate unboiled at 20 µg

Lane 2:

Mouse brain lysate boiled at 20 µg

Lane 3:

Rat brain lysate unboiled at 20 µg

Lane 4:

Rat brain lysate boiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 102 kDa

false

Exposure time: 1s

Carrier free

Anti-ATP1A2 antibody [EPR11896(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11896(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP1A2 also known as the Na+/K+ ATPase alpha-2 subunit is integral in the maintenance of the sodium and potassium gradient across the plasma membrane. This protein with a mass around 113 kDa functions as part of the Na+/K+ ATPase pump and is essential for cellular ion homeostasis. ATP1A2 is expressed in various tissues with high expression noted in the brain heart and skeletal muscle. The pump activity provides necessary conditions for maintaining resting membrane potential and supporting cellular processes such as nutrient transport.

Biological function summary

ATP1A2 contributes to neuronal excitability and muscle contraction. It forms a part of the Na+/K+ ATPase enzyme complex which plays a significant role in neurotransmitter release and action potential propagation in neurons. This action is critical in both central and peripheral nervous systems. The interaction of ATP1A2 with intracellular signaling pathways also influences cardiac muscle function impacting heart rate and contractility.

Pathways

ATP1A2 plays an essential role in the regulation of certain vital biochemical pathways like the electrical excitability in nerve cells and the cardiac action potential pathways. These pathways coordinate a variety of physiological responses and maintain cellular energetics. The protein pathways involving ATP1A2 often intersect with those of other ion channels and transporters such as the NCX (sodium-calcium exchanger) which further modulate cellular ion exchange and signaling processes.

ATP1A2 mutations have associations with conditions such as familial hemiplegic migraine type 2 and alternating hemiplegia of childhood. These disorders often result from disrupted ion homeostasis leading to abnormal neural activity. The altered function of ATP1A2 in these diseases may also show interaction with proteins involved in neurotransmitter regulation such as those involved in the glutamatergic and GABAergic signaling pathways potentially exacerbating neural dysfunction and symptoms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients.

See full target information ATP1A2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 30:101398 PubMed39790485

2025

Porcine pericardial decellularized matrix bilayer patch containing adipose stem cell-derived exosomes for the treatment of diabetic wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liang,Huiting Wu,Lindan Tan,Xiaoyu Meng,Wanwen Dang,Meng Han,Yonghuan Zhen,Haifeng Chen,Hongsen Bi,Yang An

The journal of headache and pain 22:92 PubMed34384358

2021

Functional correlation of ATP1A2 mutations with phenotypic spectrum: from pure hemiplegic migraine to its variant forms.

Applications

Unspecified application

Species

Unspecified reactive species

Yingji Li,Wenjing Tang,Li Kang,Shanshan Kong,Zhao Dong,Dengfa Zhao,Ruozhuo Liu,Shengyuan Yu

Molecular pharmacology 96:138-147 PubMed31189668

2019

Generation and Characterization of an Humanized Mouse Model ( ) with Knockout Capability.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Krohn,Viktoria Zoufal,Severin Mairinger,Thomas Wanek,Kristin Paarmann,Thomas Brüning,Ivan Eiriz,Mirjam Brackhan,Oliver Langer,Jens Pahnke

American journal of physiology. Cell physiology 314:C627-C639 PubMed29365273

2018

P2Y receptor regulation of K2P channels that facilitate K secretion by human mammary epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yotesawee Srisomboon,Nathan A Zaidman,Peter J Maniak,Chatsri Deachapunya,Scott M O'Grady

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP1A2 antibody [EPR11896(B)]

Western blot - Anti-ATP1A2 antibody [EPR11896(B)] (AB166888)

Western blot - Anti-ATP1A2 antibody [EPR11896(B)] (AB166888)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise