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AB249372

Anti-ATP1A2 antibody [EPR11896(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal AT1A2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

KIAA0778, ATP1A2, Sodium/potassium-transporting ATPase subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, Sodium pump subunit alpha-2

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11896(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249372 is the carrier-free version of ab166888.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP1A2 also known as the Na+/K+ ATPase alpha-2 subunit is integral in the maintenance of the sodium and potassium gradient across the plasma membrane. This protein with a mass around 113 kDa functions as part of the Na+/K+ ATPase pump and is essential for cellular ion homeostasis. ATP1A2 is expressed in various tissues with high expression noted in the brain heart and skeletal muscle. The pump activity provides necessary conditions for maintaining resting membrane potential and supporting cellular processes such as nutrient transport.
Biological function summary

ATP1A2 contributes to neuronal excitability and muscle contraction. It forms a part of the Na+/K+ ATPase enzyme complex which plays a significant role in neurotransmitter release and action potential propagation in neurons. This action is critical in both central and peripheral nervous systems. The interaction of ATP1A2 with intracellular signaling pathways also influences cardiac muscle function impacting heart rate and contractility.

Pathways

ATP1A2 plays an essential role in the regulation of certain vital biochemical pathways like the electrical excitability in nerve cells and the cardiac action potential pathways. These pathways coordinate a variety of physiological responses and maintain cellular energetics. The protein pathways involving ATP1A2 often intersect with those of other ion channels and transporters such as the NCX (sodium-calcium exchanger) which further modulate cellular ion exchange and signaling processes.

ATP1A2 mutations have associations with conditions such as familial hemiplegic migraine type 2 and alternating hemiplegia of childhood. These disorders often result from disrupted ion homeostasis leading to abnormal neural activity. The altered function of ATP1A2 in these diseases may also show interaction with proteins involved in neurotransmitter regulation such as those involved in the glutamatergic and GABAergic signaling pathways potentially exacerbating neural dysfunction and symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients.
See full target information ATP1A2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com