AB182571

Anti-ATP1A3 antibody [EPR14138]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal ATP1A3 antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-3, Na(+)/K(+) ATPase alpha-3 subunit, Na(+)/K(+) ATPase alpha(III) subunit, Sodium pump subunit alpha-3, ATP1A3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (AB182571)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (AB182571)

Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling ATP1A3 with purified ab182571 at 1/50 (5.2 μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Lab

Western blot - Anti-ATP1A3 antibody [EPR14138] (AB182571)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATP1A3 antibody [EPR14138] (ab182571) at 1/1000 dilution

Lane 1:

Mouse brain lysate, unboiled at 20 µg

Lane 2:

Mouse brain lysate, boiled at 20 µg

Lane 3:

Rat brain lysate, unboiled at 20 µg

Lane 4:

Rat brain lysate, boiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 111 kDa

Observed band size: 102 kDa

false

Exposure time: 3s

Carrier free

Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14138

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20 - 1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP1A3 also known as the alpha3 subunit of the sodium-potassium ATPase is an ion pump that functions to maintain the electrochemical gradients of sodium and potassium ions across the plasma membrane. It is a protein with a molecular mass of approximately 110 kDa. ATP1A3 expression is found mainly in neurons of the central nervous system. By actively transporting three sodium ions out of the cell and two potassium ions into the cell ATP1A3 helps sustain the resting potential essential for neuronal excitability and signaling.

Biological function summary

The sodium-potassium ATPase where ATP1A3 serves acts as a vital component in maintaining ion homeostasis. This protein resides in cell membranes and forms a part of the larger complex required for normal neuron function. Its activity also influences other cell functions like cell volume regulation and signal transduction processes. The alpha3 subunit specifically exhibits specialized roles in neuronal tissues where rapid Na+ and K+ exchange is necessary contributing significantly to nerve impulse transmission.

Pathways

The function of ATP1A3 is integral in the maintenance of the membrane potential which is an important part of many physiological pathways including the action potential propagation in neurons. Another pathway where its activity is significant involves synaptic vesicle cycling which depends on ATP1A3 to maintain ionic conditions for synaptic transmission. Proteins such as the beta subunit of the sodium-potassium ATPase ATP1B1 closely associate with ATP1A3 and modulate its activity and stability.

Mutations in ATP1A3 have associations with neurological conditions such as Alternating Hemiplegia of Childhood (AHC) and Rapid-Onset Dystonia-Parkinsonism (RDP). These disorders are characterized by sudden severe disruptions in motor function that relate to impaired ion gradients caused by dysfunctional ATP1A3. In AHC the disrupted interaction of ATP1A3 with other ion pumps like ATP1A2 exacerbates neurological function deficits.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

See full target information ATP1A3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:363 PubMed38796484

2024

Neuronal autosis is Na/K-ATPase alpha 3-dependent and involved in hypoxic-ischemic neuronal death.

Applications

Unspecified application

Species

Unspecified reactive species

Pauline Depierre,Vanessa Ginet,Anita C Truttmann,Julien Puyal

Nature communications 15:2189 PubMed38467605

2024

PCDHA9 as a candidate gene for amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhong,Chaodong Wang,Dan Zhang,Xiaoli Yao,Quanzhen Zhao,Xusheng Huang,Feng Lin,Chun Xue,Yaqing Wang,Ruojie He,Xu-Ying Li,Qibin Li,Mingbang Wang,Shaoli Zhao,Shabbir Khan Afridi,Wenhao Zhou,Zhanjun Wang,Yanming Xu,Zhiheng Xu

Cell reports 37:109899 PubMed34706226

2021

Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Ulferts,Elena Marcassa,Lewis Timimi,Liam Changwoo Lee,Andrew Daley,Beatriz Montaner,Suzanne Dawn Turner,Oliver Florey,John Kenneth Baillie,Rupert Beale

JCI insight 5: PubMed31941841

2020

Interaction between the autophagy protein Beclin 1 and Na+,K+-ATPase during starvation, exercise, and ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Álvaro F Fernández,Yang Liu,Vanessa Ginet,Mingjun Shi,Jihoon Nah,Zhongju Zou,Anwu Zhou,Bruce A Posner,Guanghua Xiao,Marion Tanguy,Valérie Paradis,Junichi Sadoshima,Pierre-Emmanuel Rautou,Julien Puyal,Ming Chang Hu,Beth Levine

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP1A3 antibody [EPR14138]

Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] (AB182571)

Western blot - Anti-ATP1A3 antibody [EPR14138] (AB182571)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise