Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (AB250624)

Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling ATP1A3 with purified ab182571 at 1/50 (5.2 μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182571).

• WB

Lab

Western blot - Anti-ATP1A3 antibody [EPR14138] - BSA and Azide free (AB250624)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182571). Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATP1A3 antibody [EPR14138] (<a href='/en-us/products/primary-antibodies/atp1a3-antibody-epr14138-ab182571'>ab182571</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate, unboiled at 20 µg

Lane 2:

Mouse brain lysate, boiled at 20 µg

Lane 3:

Rat brain lysate, unboiled at 20 µg

Lane 4:

Rat brain lysate, boiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 111 kDa

Observed band size: 102 kDa

false

Exposure time: 3s