Mouse Monoclonal ATP1B1 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples. Cited in 12 publications.
Images
![Western blot - Anti-ATP1B1 antibody [464.8] (AB8344), expandable thumbnail](https://content.abcam.com/products/images/atp1b1-antibody-4648-ab8344--western-blot-img97026.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATP1B1 antibody [464.8] (AB8344)")
![Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (AB8344), expandable thumbnail](https://content.abcam.com/products/images/atp1b1-antibody-4648-ab8344--flow-cytometry-intracellular-img50264.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (AB8344)")
Publications
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- European journal of oral sciences 122:21-62013Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Wen,Rodrigo S Lacruz,Charles E Smith,Michael L PainePubMed 24313748
- PloS one 8:e707502013Ca²⁺/calmodulin-dependent protein kinase II contributes to hypoxic ischemic cell death in neonatal hippocampal slice cultures.
- Gut 63:808-172013Farnesoid X receptor agonists attenuate colonic epithelial secretory function and prevent experimental diarrhoea in vivo.
- Free radical biology & medicine 53:1139-512012Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures.
- EMBO molecular medicine 2:63-752010Role of the WNK-activated SPAK kinase in regulating blood pressure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatema H Rafiqi,Annie Mercier Zuber,Mark Glover,Ciaran Richardson,Stewart Fleming,Sofija Jovanović,Aleksandar Jovanović,Kevin M O'Shaughnessy,Dario R AlessiPubMed 20091762
- Reproductive sciences (Thousand Oaks, Calif.) 15:366-732008Altered endothelin receptor binding in response to nitric oxide synthase inhibition in the pregnant rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark G Neerhof et. al.PubMed 18325929
- European journal of immunology 37:1194-2032007NADPH oxidase of human dendritic cells: role in Candida albicans killing and regulation by interferons, dectin-1 and CD206.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Donini,Elena Zenaro,Nicola Tamassia,Stefano DusiPubMed 17407098
- The American journal of physiology 267:F75-851994Expression and molecular regulation of Na(+)-K(+)-ATPase after renal ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS K Van Why,A S Mann,T Ardito,N J Siegel,M KashgarianPubMed 8048568
- The American journal of physiology 266:C579-891994Analysis of subunit assembly of the Na-K-ATPase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD M Fambrough,M V Lemas,M Hamrick,M Emerick,K J Renaud,E M Inman,B Hwang,K TakeyasuPubMed 8166221
- The Journal of biological chemistry 268:14342-71993Molecular requirements for the cell-surface expression of multisubunit ion-transporting ATPases. Identification of protein domains that participate in Na,K-ATPase and H,K-ATPase subunit assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC J Gottardi,M J CaplanPubMed 8390991
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | WB | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane (PubMed:19694409). Plays a role in innate immunity by enhancing virus-triggered induction of interferons (IFNs) and interferon stimulated genes (ISGs). Mechanistically, enhances the ubiquitination of TRAF3 and TRAF6 as well as the phosphorylation of TAK1 and TBK1 (PubMed:34011520). Involved in cell adhesion and establishing epithelial cell polarity.
Alternative names
ATP1B, ATP1B1, Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1
Recommended products
Mouse Monoclonal ATP1B1 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples. Cited in 12 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 464.8
- Purification technique
- Affinity purification
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP1B1 also known as the beta-1 subunit of Na+/K+ ATPase plays an essential role in ion transport across the plasma membrane. The protein works as part of the sodium-potassium pump which helps maintain the cellular electrochemical gradient. The molecular mass of ATP1B1 is approximately 35 kDa. This target is expressed in various tissues including the heart kidney and brain where it supports the critical functions of excitable tissues and epithelial cell polarization.
- Biological function summary
ATP1B1 contributes to establishing and maintaining cellular homeostasis as part of the Na+/K+ ATPase complex. This complex regulates the balance of sodium and potassium ions within cells important for nerve impulse transmission and muscle contraction. ATP1B1 interacts with the alpha subunit to modulate the enzyme’s activity and transport kinetics highlighting its role in cell volume regulation and epithelial layer development.
- Pathways
ATP1B1 is actively involved in the ion transport pathway and cellular signaling pathways such as WNT signaling. It works closely with proteins such as ATP1A1 and ATP1A2 which are different alpha subunits of the Na+/K+ ATPase. Through these pathways ATP1B1 affects cellular processes like membrane potential maintenance and intercellular communication essential for proper cellular function and organismal health.
- Associated diseases and disorders
ATP1B1 shows a link to hypertension and polycystic ovary syndrome (PCOS). Mutations or dysfunctional expression of ATP1B1 can disrupt ion gradients contributing to these conditions. The protein connects with regulatory proteins including G-protein signaling regulators which can alter cellular responses in disease contexts. Understanding ATP1B1's involvement in these disorders helps in exploring therapeutic targets and improving patient outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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2 product images
![Western blot - Anti-ATP1B1 antibody [464.8] (ab8344), expandable thumbnail](https://content.abcam.com/products/images/atp1b1-antibody-4648-ab8344--western-blot-img97026.jpg "Western blot - Anti-ATP1B1 antibody [464.8] (ab8344)")
Western blot - Anti-ATP1B1 antibody [464.8] (ab8344)
All lanes: Western blot - Anti-ATP1B1 antibody [464.8] (ab8344) at 1/500 dilution
All lanes: Human kidney tissue lysate - total protein (ab30203) at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 45 kDa
Exposure time: 4min
![Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (ab8344), expandable thumbnail](https://content.abcam.com/products/images/atp1b1-antibody-4648-ab8344--flow-cytometry-intracellular-img50264.jpg "Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (ab8344)")
Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (ab8344)
Overlay histogram showing HepG2 cells stained with ab8344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8344, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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