AB8344

Anti-ATP1B1 antibody [464.8]

Name: Anti-ATP1B1 antibody [464.8] (ab8344) | Abcam
Brand: Abcam Limited
SKU: AB8344
Price: 505 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(12 Publications)

Mouse Monoclonal ATP1B1 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples. Cited in 12 publications.

View Alternative Names

ATP1B, ATP1B1, Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1

2 Images

Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (AB8344)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (AB8344)

Overlay histogram showing HepG2 cells stained with ab8344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8344, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Western blot - Anti-ATP1B1 antibody [464.8] (AB8344)

All lanes:

Western blot - Anti-ATP1B1 antibody [464.8] (ab8344) at 1/500 dilution

All lanes:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 20 µg

Predicted band size: 35 kDa

Observed band size: 45 kDa

true

Exposure time: 4min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

464.8

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP1B1 also known as the beta-1 subunit of Na+/K+ ATPase plays an essential role in ion transport across the plasma membrane. The protein works as part of the sodium-potassium pump which helps maintain the cellular electrochemical gradient. The molecular mass of ATP1B1 is approximately 35 kDa. This target is expressed in various tissues including the heart kidney and brain where it supports the critical functions of excitable tissues and epithelial cell polarization.

Biological function summary

ATP1B1 contributes to establishing and maintaining cellular homeostasis as part of the Na+/K+ ATPase complex. This complex regulates the balance of sodium and potassium ions within cells important for nerve impulse transmission and muscle contraction. ATP1B1 interacts with the alpha subunit to modulate the enzyme’s activity and transport kinetics highlighting its role in cell volume regulation and epithelial layer development.

Pathways

ATP1B1 is actively involved in the ion transport pathway and cellular signaling pathways such as WNT signaling. It works closely with proteins such as ATP1A1 and ATP1A2 which are different alpha subunits of the Na+/K+ ATPase. Through these pathways ATP1B1 affects cellular processes like membrane potential maintenance and intercellular communication essential for proper cellular function and organismal health.

ATP1B1 shows a link to hypertension and polycystic ovary syndrome (PCOS). Mutations or dysfunctional expression of ATP1B1 can disrupt ion gradients contributing to these conditions. The protein connects with regulatory proteins including G-protein signaling regulators which can alter cellular responses in disease contexts. Understanding ATP1B1's involvement in these disorders helps in exploring therapeutic targets and improving patient outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane (PubMed : 19694409). Plays a role in innate immunity by enhancing virus-triggered induction of interferons (IFNs) and interferon stimulated genes (ISGs). Mechanistically, enhances the ubiquitination of TRAF3 and TRAF6 as well as the phosphorylation of TAK1 and TBK1 (PubMed : 34011520).. Involved in cell adhesion and establishing epithelial cell polarity.

See full target information ATP1B1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

European journal of oral sciences 122:21-6 PubMed24313748

2013

Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wen,Rodrigo S Lacruz,Charles E Smith,Michael L Paine

PloS one 8:e70750 PubMed23976956

2013

Ca²⁺/calmodulin-dependent protein kinase II contributes to hypoxic ischemic cell death in neonatal hippocampal slice cultures.

Applications

Species

Rat

Qing Lu,Valerie A Harris,Xutong Sun,Yali Hou,Stephen M Black

Gut 63:808-17 PubMed23916961

2013

Farnesoid X receptor agonists attenuate colonic epithelial secretory function and prevent experimental diarrhoea in vivo.

Applications

Species

Mouse

Magdalena S Mroz,Niamh Keating,Joseph B Ward,Rafiquel Sarker,Silvie Amu,Gabriella Aviello,Mark Donowitz,Padraic G Fallon,Stephen J Keely

Free radical biology & medicine 53:1139-51 PubMed22728269

2012

Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures.

Applications

Species

Unspecified reactive species

Qing Lu,Mark S Wainwright,Valerie A Harris,Saurabh Aggarwal,Yali Hou,Thomas Rau,David J Poulsen,Stephen M Black

EMBO molecular medicine 2:63-75 PubMed20091762

2010

Role of the WNK-activated SPAK kinase in regulating blood pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Fatema H Rafiqi,Annie Mercier Zuber,Mark Glover,Ciaran Richardson,Stewart Fleming,Sofija Jovanović,Aleksandar Jovanović,Kevin M O'Shaughnessy,Dario R Alessi

Reproductive sciences (Thousand Oaks, Calif.) 15:366-73 PubMed18325929

2008

Altered endothelin receptor binding in response to nitric oxide synthase inhibition in the pregnant rat.

Applications

Unspecified application

Species

Unspecified reactive species

Mark G Neerhof,Tamas Jilling,Sylvia Synowiec,Saira Khan,Larry G Thaete

European journal of immunology 37:1194-203 PubMed17407098

2007

NADPH oxidase of human dendritic cells: role in Candida albicans killing and regulation by interferons, dectin-1 and CD206.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Donini,Elena Zenaro,Nicola Tamassia,Stefano Dusi

The American journal of physiology 267:F75-85 PubMed8048568

1994

Expression and molecular regulation of Na(+)-K(+)-ATPase after renal ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

S K Van Why,A S Mann,T Ardito,N J Siegel,M Kashgarian

The American journal of physiology 266:C579-89 PubMed8166221

1994

Analysis of subunit assembly of the Na-K-ATPase.

Applications

Unspecified application

Species

Unspecified reactive species

D M Fambrough,M V Lemas,M Hamrick,M Emerick,K J Renaud,E M Inman,B Hwang,K Takeyasu

The Journal of biological chemistry 268:14342-7 PubMed8390991

1993

Molecular requirements for the cell-surface expression of multisubunit ion-transporting ATPases. Identification of protein domains that participate in Na,K-ATPase and H,K-ATPase subunit assembly.

Applications

Unspecified application

Species

Unspecified reactive species

C J Gottardi,M J Caplan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP1B1 antibody [464.8]

Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (AB8344)

Western blot - Anti-ATP1B1 antibody [464.8] (AB8344)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise