AB96292

Anti-ATP1B1 antibody

(1 Review)

(1 Publication)

Rabbit Polyclonal ATP1B1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP1B1 aa 50 to C-terminus.

View Alternative Names

ATP1B, ATP1B1, Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody (AB96292)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody (AB96292)

ab96292 detects ATP1B1 protein at membrane on Cal27 (human) xenograft by immunohistochemical analysis. Sample : Paraffin-embedded Cal27 xenograft. Antibody dilution : 1 : 500

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody (AB96292)

ab96292 detects ATP1B1 protein at membrane on human hepatoma by immunohistochemcial analysis. Sample : Paraffin-embedded hepatoma tissue. Antibody dilution : 1 : 500

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody (AB96292)

ab96292, at 1/100 dilution, staining beta Sodium Potassium ATPase in paraffin-embedded MDAMB468 (human) xenograft by Immunohistochemistry.

Supplier Data

Western blot - Anti-ATP1B1 antibody (AB96292)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ATP1B1 antibody (ab96292) at 1/1000 dilution

All lanes:

Human brain tissue extract at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-ATP1B1 antibody (AB96292)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ATP1B1 antibody (ab96292) at 1/1000 dilution

All lanes:

Mouse brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ATP1B1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05026

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "Optimal dilutions/concentrations should be determined by the researcher", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Optimal dilutions/concentrations should be determined by the researcher" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "Optimal dilutions/concentrations should be determined by the researcher", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "Optimal dilutions/concentrations should be determined by the researcher", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP1B1 also known as the beta-1 subunit of Na+/K+ ATPase plays an essential role in ion transport across the plasma membrane. The protein works as part of the sodium-potassium pump which helps maintain the cellular electrochemical gradient. The molecular mass of ATP1B1 is approximately 35 kDa. This target is expressed in various tissues including the heart kidney and brain where it supports the critical functions of excitable tissues and epithelial cell polarization.

Biological function summary

ATP1B1 contributes to establishing and maintaining cellular homeostasis as part of the Na+/K+ ATPase complex. This complex regulates the balance of sodium and potassium ions within cells important for nerve impulse transmission and muscle contraction. ATP1B1 interacts with the alpha subunit to modulate the enzyme’s activity and transport kinetics highlighting its role in cell volume regulation and epithelial layer development.

Pathways

ATP1B1 is actively involved in the ion transport pathway and cellular signaling pathways such as WNT signaling. It works closely with proteins such as ATP1A1 and ATP1A2 which are different alpha subunits of the Na+/K+ ATPase. Through these pathways ATP1B1 affects cellular processes like membrane potential maintenance and intercellular communication essential for proper cellular function and organismal health.

ATP1B1 shows a link to hypertension and polycystic ovary syndrome (PCOS). Mutations or dysfunctional expression of ATP1B1 can disrupt ion gradients contributing to these conditions. The protein connects with regulatory proteins including G-protein signaling regulators which can alter cellular responses in disease contexts. Understanding ATP1B1's involvement in these disorders helps in exploring therapeutic targets and improving patient outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane (PubMed : 19694409). Plays a role in innate immunity by enhancing virus-triggered induction of interferons (IFNs) and interferon stimulated genes (ISGs). Mechanistically, enhances the ubiquitination of TRAF3 and TRAF6 as well as the phosphorylation of TAK1 and TBK1 (PubMed : 34011520).. Involved in cell adhesion and establishing epithelial cell polarity.

See full target information ATP1B1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Society of Nephrology : JA 26:400-9 PubMed25012180

2014

Physical and functional links between anion exchanger-1 and sodium pump.

Applications

Unspecified application

Species

Human

Ya Su,Rafia S Al-Lamki,Katherine G Blake-Palmer,Alison Best,Zoe J Golder,Aiwu Zhou,Fiona E Karet Frankl

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP1B1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody (AB96292)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody (AB96292)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody (AB96292)

Western blot - Anti-ATP1B1 antibody (AB96292)

Western blot - Anti-ATP1B1 antibody (AB96292)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise