AB251176

Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ATP1B1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

View Alternative Names

ATP1B, ATP1B1, Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

This data was developed using ab193669, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling ATP1B1 with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

This data was developed using ab193669, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ATP1B1 with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

This data was developed using ab193669, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP1B1 with ab193669 at 1/100 dilution (7.8μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

This data was developed using ab193669, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATP1B1 antibody [EPR12195] (<a href='/en-us/products/primary-antibodies/atp1b1-antibody-epr12195-ab193669'>ab193669</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 45-50 kDa

false

Supplier Data

Western blot - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

This data was developed using ab193669, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATP1B1 antibody [EPR12195] (<a href='/en-us/products/primary-antibodies/atp1b1-antibody-epr12195-ab193669'>ab193669</a>) at 1/10000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 45-50 kDa

false

Supplier Data

Western blot - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

This data was developed using ab193669, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATP1B1 antibody [EPR12195] (<a href='/en-us/products/primary-antibodies/atp1b1-antibody-epr12195-ab193669'>ab193669</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 45-50 kDa

false

Unconjugated

Anti-ATP1B1 antibody [EPR12195]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12195

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251176 is the carrier-free version of ab193669.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP1B1 also known as the beta-1 subunit of Na+/K+ ATPase plays an essential role in ion transport across the plasma membrane. The protein works as part of the sodium-potassium pump which helps maintain the cellular electrochemical gradient. The molecular mass of ATP1B1 is approximately 35 kDa. This target is expressed in various tissues including the heart kidney and brain where it supports the critical functions of excitable tissues and epithelial cell polarization.

Biological function summary

ATP1B1 contributes to establishing and maintaining cellular homeostasis as part of the Na+/K+ ATPase complex. This complex regulates the balance of sodium and potassium ions within cells important for nerve impulse transmission and muscle contraction. ATP1B1 interacts with the alpha subunit to modulate the enzyme’s activity and transport kinetics highlighting its role in cell volume regulation and epithelial layer development.

Pathways

ATP1B1 is actively involved in the ion transport pathway and cellular signaling pathways such as WNT signaling. It works closely with proteins such as ATP1A1 and ATP1A2 which are different alpha subunits of the Na+/K+ ATPase. Through these pathways ATP1B1 affects cellular processes like membrane potential maintenance and intercellular communication essential for proper cellular function and organismal health.

ATP1B1 shows a link to hypertension and polycystic ovary syndrome (PCOS). Mutations or dysfunctional expression of ATP1B1 can disrupt ion gradients contributing to these conditions. The protein connects with regulatory proteins including G-protein signaling regulators which can alter cellular responses in disease contexts. Understanding ATP1B1's involvement in these disorders helps in exploring therapeutic targets and improving patient outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane (PubMed : 19694409). Plays a role in innate immunity by enhancing virus-triggered induction of interferons (IFNs) and interferon stimulated genes (ISGs). Mechanistically, enhances the ubiquitination of TRAF3 and TRAF6 as well as the phosphorylation of TAK1 and TBK1 (PubMed : 34011520).. Involved in cell adhesion and establishing epithelial cell polarity.

See full target information ATP1B1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

Western blot - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

Western blot - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

Western blot - Anti-ATP1B1 antibody [EPR12195] - BSA and Azide free (AB251176)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise