Rabbit Recombinant Monoclonal ATP1B2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Western blot - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (AB250809), expandable thumbnail](https://content.abcam.com/products/images/atp1b2-antibody-epr15460b-bsa-and-azide-free-ab250809--western-blot-img107244.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (AB250809)")
![Immunoprecipitation - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (AB250809), expandable thumbnail](https://content.abcam.com/products/images/atp1b2-antibody-epr15460b-bsa-and-azide-free-ab250809--immunoprecipitation-img74274.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (AB250809)")
![Western blot - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (AB250809), expandable thumbnail](https://content.abcam.com/products/images/atp1b2-antibody-epr15460b-bsa-and-azide-free-ab250809--western-blot-img41039.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (AB250809)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Mediates cell adhesion of neurons and astrocytes, and promotes neurite outgrowth.
Alternative names
Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule in glia, Sodium/potassium-dependent ATPase subunit beta-2, AMOG, ATP1B2
Recommended products
Rabbit Recombinant Monoclonal ATP1B2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15460(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250809 is the carrier-free version of Anti-ATP1B2 antibody [EPR15460(B)] ab185210.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP1B2 also known as Sodium/potassium-transporting ATPase subunit beta-2 is a protein that functions mechanically as part of the Na+/K+ pump essential for maintaining the electrochemical gradients of sodium and potassium ions across the plasma membrane. This protein is approximately 40 kDa in mass. It is particularly expressed in nervous tissues such as brain and spinal cord but can be found in many other tissues as well. ATP1B2 plays a significant role in ion transport and regulation of cell volume as well as maintaining resting potential.
- Biological function summary
ATP1B2 participates in the assembly of the Na+/K+ ATPase enzyme complex a complex vital for proper ion exchange and cellular homeostasis. It stabilizes the enzyme's alpha subunit and ensures correct localization within the cell membrane. This complex is essential for cellular processes like neuronal signaling and muscle contraction as it helps in maintaining the appropriate ion balance within the cells.
- Pathways
ATP1B2 is involved in the ion transport pathway important for maintaining cellular ion homeostasis. It is part of pathways such as the sodium reabsorption in kidneys and nerve transmission. In these pathways ATP1B2 works closely with proteins like ATP1A1 the alpha subunit of the Na+/K+ ATPase helping to facilitate the transport of sodium out of and potassium into the cells which is critical for many physiological processes.
- Associated diseases and disorders
ATP1B2 has been linked to conditions such as epilepsy and certain types of congenital heart disease. In epilepsy dysfunctional ion transport due to anomalies in ATP1B2 can lead to abnormal neuronal firing. Another related protein ATP1A2 also plays a part in neurological disorders through similar ion transport mechanisms. In congenital heart disease disruptions in the Na+/K+ pump imaging can affect heart function demonstrating ATP1B2's role in maintaining cardiovascular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (ab250809), expandable thumbnail](https://content.abcam.com/products/images/atp1b2-antibody-epr15460b-bsa-and-azide-free-ab250809--western-blot-img107244.jpg "Western blot - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (ab250809)")
Western blot - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (ab250809)
This data was developed using Anti-ATP1B2 antibody [EPR15460(B)] ab185210, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ATP1B2 antibody [EPR15460(B)] (Anti-ATP1B2 antibody [EPR15460(B)] ab185210) at 1/5000 dilution
All lanes: Y79 (Human retinoblastoma cell line) cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
![Immunoprecipitation - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (ab250809), expandable thumbnail](https://content.abcam.com/products/images/atp1b2-antibody-epr15460b-bsa-and-azide-free-ab250809--immunoprecipitation-img74274.jpg "Immunoprecipitation - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (ab250809)")
Immunoprecipitation - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (ab250809)
This data was developed using Anti-ATP1B2 antibody [EPR15460(B)] ab185210, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human cerebellum lysate immunoprecipitated using Anti-ATP1B2 antibody [EPR15460(B)] ab185210 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.All lanes: Immunoprecipitation - Anti-ATP1B2 antibody [EPR15460(B)] (Anti-ATP1B2 antibody [EPR15460(B)] ab185210)
Predicted band size: 33 kDa
![Western blot - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (ab250809), expandable thumbnail](https://content.abcam.com/products/images/atp1b2-antibody-epr15460b-bsa-and-azide-free-ab250809--western-blot-img41039.jpg "Western blot - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (ab250809)")
Western blot - Anti-ATP1B2 antibody [EPR15460(B)] - BSA and Azide free (ab250809)
This data was developed using Anti-ATP1B2 antibody [EPR15460(B)] ab185210, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ATP1B2 antibody [EPR15460(B)] (Anti-ATP1B2 antibody [EPR15460(B)] ab185210) at 1/20000 dilution
All lanes: Human cerebellum lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
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