Rabbit Polyclonal ATP1B3 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Sodium/potassium-transporting ATPase subunit beta-3 aa 50 to C-terminus.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Sodium/potassium-transporting ATPase subunit beta-3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P97370
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5-2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5-20 µg/mL | Notes - |
Associated Products
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Target data
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
Alternative names
CD298, Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, ATPB-3, Atp1b3
Recommended products
Rabbit Polyclonal ATP1B3 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Sodium/potassium-transporting ATPase subunit beta-3 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Sodium/potassium-transporting ATPase subunit beta-3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P97370
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab231671 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP1B3 also known as ATPase Na+/K+ transporting subunit beta-3 is a protein involved in ion transport. It has a molecular mass of around 31 kDa. ATP1B3 is part of the Na+/K+ ATPase complex which maintains electrochemical gradients across cell membranes by pumping sodium out of and potassium into the cell using ATP as energy source. It is widely expressed in different tissues with notable expression in the kidneys brain and heart. This protein plays a functional role by serving as an accessory subunit that stabilizes and regulates the enzyme complex.
- Biological function summary
ATP1B3 supports the proper functioning of the Na+/K+ ATPase by influencing the conformation and activity of the enzyme complex. The protein ensures that the ion gradients needed for basic cellular activities such as electrical excitability and nutrient uptake are preserved. It along with alpha subunits forms heterodimeric complexes required for ion exchange. Its presence ensures that the complex works effectively supporting various physiological functions across multiple organ systems.
- Pathways
It aligns with the ion transport and signal transduction pathways related to maintaining cellular homeostasis. ATP1B3 interacts with other proteins like the alpha-1 subunit of the Na+/K+ ATPase important for regulating membrane potentials. The protein plays a role in the Wnt signaling pathway influencing processes such as cell proliferation and migration. Understanding ATP1B3 in these pathways helps unveil its broad functional implications.
- Associated diseases and disorders
ATP1B3 associates with both cardiovascular diseases and neurological disturbances. Mutations or altered expression of this protein disrupt ion exchange which can lead to heart failure or epilepsy. In these conditions blurred regulation of ATP1B3 affects the Na+/K+ ATPase complex composed of alpha and gamma subunits. This can result in imbalanced ion homeostasis emphasizing the importance of ATP1B3 in maintaining normal physiological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-ATP1B3 antibody (ab231671)
All lanes: Western blot - Anti-ATP1B3 antibody (ab231671) at 2 µg/mL
All lanes: Recombinant mouse ATP1B3
SecondaryAll lanes: HRP-Linked Goat anti-Rabbit IgG at 0.2 µg/mL
Predicted band size: 32 kDa
Western blot - Anti-ATP1B3 antibody (ab231671)
All lanes: Western blot - Anti-ATP1B3 antibody (ab231671) at 2 µg
All lanes: Mouse heart lysate
SecondaryAll lanes: HRP-Linked Goat anti-Rabbit IgG at 0.2 µg/mL
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody (ab231671)
Formalin-fixed, paraffin-embedded mouse skeletal muscle tissue stained for ATP1B3 using ab231671 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody (ab231671)
Formalin-fixed, paraffin-embedded mouse skeletal muscle tissue stained for ATP1B3 using ab231671 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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