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AB67409

Anti-ATP1B3 antibody

0

(1 Review)

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(2 Publications)

Mouse Polyclonal ATP1B3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Sodium/potassium-transporting ATPase subunit beta-3.

View Alternative Names

CD298, Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, ATPB-3, ATP1B3

1 Images
Western blot - Anti-ATP1B3 antibody (AB67409)
  • WB

Unknown

Western blot - Anti-ATP1B3 antibody (AB67409)

All lanes:

Western blot - Anti-ATP1B3 antibody (ab67409) at 1/500 dilution

Lane 1:

ATP1B3 293T transfected lysate at 25 µg

Lane 2:

Non transfected 293T lysate at 25 µg

Secondary

All lanes:

Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 32 kDa

Observed band size: 35 kDa,36 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Sodium/potassium-transporting ATPase subunit beta-3.

P54709

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Alternative Products

Primary Antibodies

AB137055

Anti-ATP1B3 antibody [EPR8981]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

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  • 0
Primary Antibodies

AB231671

Anti-ATP1B3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody (AB231671)

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB76020

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (AB76020)

  • 5
  • 18
Primary Antibodies

AB193669

Anti-ATP1B1 antibody [EPR12195]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B1 antibody [EPR12195] (AB193669)

  • 0
  • 0
Primary Antibodies

AB307727

Anti-ALP antibody [EPR27191-14] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALP antibody [EPR27191-14] - BSA and Azide free (AB307727)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP1B3 also known as ATPase Na+/K+ transporting subunit beta-3 is a protein involved in ion transport. It has a molecular mass of around 31 kDa. ATP1B3 is part of the Na+/K+ ATPase complex which maintains electrochemical gradients across cell membranes by pumping sodium out of and potassium into the cell using ATP as energy source. It is widely expressed in different tissues with notable expression in the kidneys brain and heart. This protein plays a functional role by serving as an accessory subunit that stabilizes and regulates the enzyme complex.
Biological function summary

ATP1B3 supports the proper functioning of the Na+/K+ ATPase by influencing the conformation and activity of the enzyme complex. The protein ensures that the ion gradients needed for basic cellular activities such as electrical excitability and nutrient uptake are preserved. It along with alpha subunits forms heterodimeric complexes required for ion exchange. Its presence ensures that the complex works effectively supporting various physiological functions across multiple organ systems.

Pathways

It aligns with the ion transport and signal transduction pathways related to maintaining cellular homeostasis. ATP1B3 interacts with other proteins like the alpha-1 subunit of the Na+/K+ ATPase important for regulating membrane potentials. The protein plays a role in the Wnt signaling pathway influencing processes such as cell proliferation and migration. Understanding ATP1B3 in these pathways helps unveil its broad functional implications.

ATP1B3 associates with both cardiovascular diseases and neurological disturbances. Mutations or altered expression of this protein disrupt ion exchange which can lead to heart failure or epilepsy. In these conditions blurred regulation of ATP1B3 affects the Na+/K+ ATPase complex composed of alpha and gamma subunits. This can result in imbalanced ion homeostasis emphasizing the importance of ATP1B3 in maintaining normal physiological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
See full target information Sodium/potassium-transporting ATPase subunit beta-3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 294:6375-6386 PubMed30792309

2019

A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na/K-ATPase in brain microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Hong Zhang,Dong-Xin Liu,Li Wang,Yu-Hua Li,Yan-Hua Wang,Hu Zhang,Zheng-Kang Su,Wen-Gang Fang,Xiao-Xue Qin,De-Shu Shang,Bo Li,Xiao-Ning Han,Wei-Dong Zhao,Yu-Hua Chen

The Journal of biological chemistry 291:4754-62 PubMed26694617

2015

ATP1B3 Protein Modulates the Restriction of HIV-1 Production and Nuclear Factor κ Light Chain Enhancer of Activated B Cells (NF-κB) Activation by BST-2.

Applications

Unspecified application

Species

Unspecified reactive species

Hironori Nishitsuji,Ryuichi Sugiyama,Makoto Abe,Hiroshi Takaku
View all publications
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Product promise

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