AB137055

Anti-ATP1B3 antibody [EPR8981]

RabMAb
Recombinant
What is this?

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(10 Publications)

Rabbit Recombinant Monoclonal ATP1B3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 10 publications.

View Alternative Names

CD298, Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, ATPB-3, ATP1B3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ATP1B3 with ab137055 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labelling ATP1B3 with ab137055 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ATP1B3 antibody [EPR8981] (AB137055)

All lanes:

Western blot - Anti-ATP1B3 antibody [EPR8981] (ab137055) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 32 kDa

Observed band size: 40 kDa

false

Carrier free

Anti-ATP1B3 antibody [EPR8981] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8981

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP1B3 also known as ATPase Na+/K+ transporting subunit beta-3 is a protein involved in ion transport. It has a molecular mass of around 31 kDa. ATP1B3 is part of the Na+/K+ ATPase complex which maintains electrochemical gradients across cell membranes by pumping sodium out of and potassium into the cell using ATP as energy source. It is widely expressed in different tissues with notable expression in the kidneys brain and heart. This protein plays a functional role by serving as an accessory subunit that stabilizes and regulates the enzyme complex.

Biological function summary

ATP1B3 supports the proper functioning of the Na+/K+ ATPase by influencing the conformation and activity of the enzyme complex. The protein ensures that the ion gradients needed for basic cellular activities such as electrical excitability and nutrient uptake are preserved. It along with alpha subunits forms heterodimeric complexes required for ion exchange. Its presence ensures that the complex works effectively supporting various physiological functions across multiple organ systems.

Pathways

It aligns with the ion transport and signal transduction pathways related to maintaining cellular homeostasis. ATP1B3 interacts with other proteins like the alpha-1 subunit of the Na+/K+ ATPase important for regulating membrane potentials. The protein plays a role in the Wnt signaling pathway influencing processes such as cell proliferation and migration. Understanding ATP1B3 in these pathways helps unveil its broad functional implications.

ATP1B3 associates with both cardiovascular diseases and neurological disturbances. Mutations or altered expression of this protein disrupt ion exchange which can lead to heart failure or epilepsy. In these conditions blurred regulation of ATP1B3 affects the Na+/K+ ATPase complex composed of alpha and gamma subunits. This can result in imbalanced ion homeostasis emphasizing the importance of ATP1B3 in maintaining normal physiological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.

See full target information Sodium/potassium-transporting ATPase subunit beta-3

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:121 PubMed38167556

2024

Unraveling the surface marker signature of cell-derived vesicles via proteome analysis and nanoparticle flow cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Chong Lau,Ilaria Passalacqua,Jik-Han Jung,Yerim Kwon,Davide Zocco,Sung-Soo Park,Seung Wook Oh

Nature communications 13:2167 PubMed35443762

2022

Single cell transcriptomic analysis reveals cellular diversity of murine esophageal epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Faujul Kabir,Adam L Karami,Ricardo Cruz-Acuña,Alena Klochkova,Reshu Saxena,Anbin Mu,Mary Grace Murray,Jasmine Cruz,Annie D Fuller,Margarette H Clevenger,Kumaraswamy Naidu Chitrala,Yinfei Tan,Kelsey Keith,Jozef Madzo,Hugh Huang,Jaroslav Jelinek,Tatiana Karakasheva,Kathryn E Hamilton,Amanda B Muir,Marie-Pier Tétreault,Kelly A Whelan

Virologica Sinica : PubMed33534085

2021

ATP1B3 Restricts Hepatitis B Virus Replication Via Reducing the Expression of the Envelope Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhang,Tianhang Zheng,Xiaolei Zhou,Hong Wang,Zhaolong Li,Chen Huan,Baisong Zheng,Wenyan Zhang

Journal of medical virology 92:201-209 PubMed31556466

2019

ATP1B3 cooperates with BST-2 to promote hepatitis B virus restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Baisong Zheng,Jun Zhang,Tianhang Zheng,Hong Wang,Zhaolong Li,Chen Huan,Shanshan Ning,Yingchao Wang,Wenyan Zhang

Epigenetics & chromatin 12:46 PubMed31331374

2019

Interplay between FACT subunit SPT16 and TRIM33 can remodel chromatin at macrophage distal regulatory elements.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Ferri,Vanessa Petit,Vilma Barroca,Paul-Henri Romeo

The Journal of biological chemistry 294:6375-6386 PubMed30792309

2019

A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na/K-ATPase in brain microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Hong Zhang,Dong-Xin Liu,Li Wang,Yu-Hua Li,Yan-Hua Wang,Hu Zhang,Zheng-Kang Su,Wen-Gang Fang,Xiao-Xue Qin,De-Shu Shang,Bo Li,Xiao-Ning Han,Wei-Dong Zhao,Yu-Hua Chen

JCI insight 3: PubMed30333310

2018

Increased expression of ATP12A proton pump in cystic fibrosis airways.

Applications

Unspecified application

Species

Unspecified reactive species

Paolo Scudieri,Ilaria Musante,Emanuela Caci,Arianna Venturini,Patrizia Morelli,Christine Walter,Davide Tosi,Alessandro Palleschi,Pablo Martin-Vasallo,Isabelle Sermet-Gaudelus,Gabrielle Planelles,Gilles Crambert,Luis Jv Galietta

Oncotarget 8:84285-84299 PubMed29137423

2017

Expression of the β3 subunit of Na/K-ATPase is increased in gastric cancer and regulates gastric cancer cell progression and prognosis via the PI3/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Ru Feng,Qian Xu,Feiyue Zhang,Tong Liu,Jiang Cao,Sujuan Fei

Channels (Austin, Tex.) 11:388-398 PubMed28636485

2017

Mineralocorticoids modulate the expression of the β-3 subunit of the Na, K-ATPase in the renal collecting duct.

Applications

Unspecified application

Species

Unspecified reactive species

Macarena Rojas,Pablo Díaz,Pablo León,Alexis A Gonzalez,Magdalena González,Víctor Barrientos,Nikolay B Pestov,Rodrigo Alzamora,Luis Michea

Molecular therapy : the journal of the American So 24:1760-1770 PubMed27434591

2016

Extracellular Antibody Drug Conjugates Exploiting the Proximity of Two Proteins.

Applications

Species

Unspecified reactive species

David J Marshall,Scott S Harried,John L Murphy,Chad A Hall,Mohammed S Shekhani,Christophe Pain,Conner A Lyons,Antonella Chillemi,Fabio Malavasi,Homer L Pearce,Jon S Thorson,James R Prudent

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP1B3 antibody [EPR8981]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (AB137055)

Western blot - Anti-ATP1B3 antibody [EPR8981] (AB137055)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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