AB326700

Anti-ATP1B3 antibody [SR4028]

Recombinant
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Rabbit Recombinant Monoclonal ATP1B3 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples.

View Alternative Names

CD298, Sodium/potassium-transporting ATPase subunit beta-3, Sodium/potassium-dependent ATPase subunit beta-3, ATPB-3, ATP1B3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [SR4028] (AB326700)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [SR4028] (AB326700)

Formalin-fixed, paraffin-embedded human liver cancer tissue stained for ATP1B3 using antibody at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ATP1B3 antibody [SR4028] (AB326700)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Mouse brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 32 kDa
Observed band size : 37 kDa

Exposure time : 6 seconds

All lanes:

Western blot - Anti-ATP1B3 antibody [SR4028] (ab326700) at 1/1000 dilution

Lane 1:

Hela whole cell lysates at 20 µg

Lane 2:

Mouse brain lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 32 kDa

Observed band size: 37 kDa

false

Exposure time: 6s

Supplier Data

Western blot - Anti-ATP1B3 antibody [SR4028] (AB326700)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : ATP1B3 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-ATP1B3 antibody [SR4028] (ab326700) at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

ATP1B3 Rabbit mAb knockdown Hela cell lysate at 20 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR4028

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.

See full target information Sodium/potassium-transporting ATPase subunit beta-3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com