Rabbit Polyclonal ATP2C1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP2C1 aa 450-750.
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- Oxidative medicine and cellular longevity 2021:48050402021OM-MSCs Alleviate the Golgi Apparatus Stress Response following Cerebral Ischemia/Reperfusion Injury via the PEDF-PI3K/Akt/mTOR Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJialin He,Jianyang Liu,Yan Huang,Xiangqi Tang,Han Xiao,Zuo Liu,Zheng Jiang,Liuwang Zeng,Zhiping Hu,Ming LuPubMed 34815829
- Frontiers in cell and developmental biology 8:5865412020Olfactory Mucosa Mesenchymal Stem Cells Alleviate Cerebral Ischemia/Reperfusion Injury Golgi Apparatus Secretory Pathway Ca -ATPase Isoform1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJialin He et. al.PubMed 33195239
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ATP2C1 aa 450-750. The exact immunogen used to generate this antibody is proprietary information. Database link P98194
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway (PubMed:12707275, PubMed:16192278, PubMed:20439740, PubMed:21187401, PubMed:30923126). Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state (PubMed:16192278, PubMed:16332677, PubMed:30923126). Plays a primary role in the maintenance of Ca(2+) homeostasis in the trans-Golgi compartment with a functional impact on Golgi and post-Golgi protein sorting as well as a structural impact on cisternae morphology (PubMed:14632183, PubMed:20439740). Responsible for loading the Golgi stores with Ca(2+) ions in keratinocytes, contributing to keratinocyte differentiation and epidermis integrity (PubMed:10615129, PubMed:14632183, PubMed:20439740). Participates in Ca(2+) and Mn(2+) ions uptake into the Golgi store of hippocampal neurons and regulates protein trafficking required for neural polarity (By similarity). May also play a role in the maintenance of Ca(2+) and Mn(2+) homeostasis and signaling in the cytosol while preventing cytotoxicity (PubMed:21187401).
Alternative names
KIAA1347, PMR1L, HUSSY-28, ATP2C1, Calcium-transporting ATPase type 2C member 1, ATPase 2C1, ATP-dependent Ca(2+) pump PMR1, Ca(2+)/Mn(2+)-ATPase 2C1, Secretory pathway Ca(2+)-transporting ATPase type 1, SPCA1
Recommended products
Rabbit Polyclonal ATP2C1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP2C1 aa 450-750.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ATP2C1 aa 450-750. The exact immunogen used to generate this antibody is proprietary information. Database link P98194
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP2C1 also known as Secretory Pathway Calcium ATPase 1 (SPCA1) is an essential calcium pump. It has a molecular mass of approximately 115 kDa. ATP2C1 is located on the Golgi membrane and participates in maintaining calcium and manganese ion homeostasis by transporting these ions from the cytosol into the Golgi apparatus. It shows expression in various tissues with high levels found in epithelial cells.
- Biological function summary
The efficient functioning of ATP2C1 regulates calcium ion concentration within the Golgi impacting protein sorting and processing. This protein contributes to cellular signaling and is a part of a broader family of P-type ATPases. Although ATP2C1 does not form part of a complex its activity indirectly influences the function of other calcium-binding proteins through its ion transport role.
- Pathways
ATP2C1 participates in key calcium homeostasis pathways ensuring cellular functions related to calcium-dependent processes. It plays a role in the intracellular signaling pathway that involves calcium-regulated gene expression. ATP2C1 is functionally linked to proteins such as STIM1 and Orai1 which are critical in the process of calcium reabsorption and signaling.
- Associated diseases and disorders
Dysfunctional ATP2C1 activity associates with Hailey-Hailey disease a genetic disorder causing recurring blisters and erosions of the skin. Mutations in the ATP2C1 gene result in impaired calcium ion transport contributing to the skin lesions. This condition shows links to the protein desmoglein-1 involved in cell adhesion suggesting disrupted adhesion due to altered calcium ion homeostasis.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-ATP2C1 antibody (ab126171)
7.5% SDS PAGE
All lanes: Western blot - Anti-ATP2C1 antibody (ab126171) at 1/2000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 101 kDa
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