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Mouse Monoclonal ATP5A antibody. Mitochondrion marker. Suitable for IHC-P, ICC/IF, Flow Cyt, WB and reacts with Human, Rat, Cow, Mouse, Drosophila melanogaster samples. Cited in 553 publications.
Alternative names=ATP synthase F1 subunit alpha, ATP5A, ATP5F1A, ATP5A1, ATP5AL2, ATPM
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested |
| Rat | Expected | Expected | Expected | Tested |
| Cow | Expected | Expected | Expected | Tested |
| Drosophila melanogaster | Expected | Expected | Expected | Expected |
| Pig | Predicted | Predicted | Predicted | Predicted |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Drosophila melanogaster | Dilution info 1.00000-10.00000 µg/mL | Notes Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-10.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Mouse | Dilution info 1/1.00000 - 1/10.00000 | Notes - |
Species Drosophila melanogaster | Dilution info 1.00000-10.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Drosophila melanogaster | Dilution info 1 µg/mL | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Cow | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal ATP5A antibody. Mitochondrion marker. Suitable for IHC-P, ICC/IF, Flow Cyt, WB and reacts with Human, Rat, Cow, Mouse, Drosophila melanogaster samples. Cited in 553 publications.
Alternative names=ATP synthase F1 subunit alpha, ATP5A, ATP5F1A, ATP5A1, ATP5AL2, ATPM
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
- Clone number
15H4C4
- Purity
IgG fraction
- Light chain type
kappa
- Concentration
- Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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