AB110273

Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker

Name: Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker (ab110273) | Abcam
Brand: Abcam Limited
SKU: AB110273
Price: 400 USD
Availability: InStock
Rating: 5 (7 reviews)

Lab Essentials
What is this?

(7 Reviews)

(84 Publications)

Anti-ATP5A antibody [7H10BD4F9] (ab110273) is a mouse monoclonal antibody detecting ATP5A in Western Blot, Flow Cytometry, ICC/IF. Suitable for Cow, Human, Mouse, Rat.

- Over 80 publications

View Alternative Names

ATP5A, ATP5A1, ATP5AL2, ATPM, ATP5F1A, ATP synthase F1 subunit alpha

3 Images

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Western blot - Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker (AB110273)

All lanes:

Western blot - Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker (ab110273) at 1 µg/mL

Lane 1:

Human heart mitochondria at 5 µg

Lane 2:

Bovine heart mitochondria at 1 µg

Lane 3:

Rat heart mitochondria at 10 µg

Lane 4:

Mouse heart mitochondria at 10 µg

Lane 5:

HepG2 mitochondria at 20 µg

Predicted band size: 60 kDa

false

Flow Cytometry - Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker (AB110273)

Flow Cyt

Unknown

Flow Cytometry - Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker (AB110273)

HL-60 cells were stained with 1 µg/mL ab110273 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker (AB110273)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker (AB110273)

Mitochondrial localization of ATP5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110273 (2 µg/ml) followed by an Alexa Fluor® 594-conjugated-goat-anti-mouse IgG2b isotype specific secondary antibody (2 µg/ml).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7H10BD4F9

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

WB, ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-ATP5A antibody [7H10BD4F9] (ab110273) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Human, Mouse, Rat samples.

What is the molecular weight of ATP5A?
Anti-ATP5A [7H10BD4F9] (ab110273) specifically detects a band for ATP5A (UniProt: P25705) at a molecular weight of 60kDa.

Trusted by the scientific community
Anti-ATP5A [7H10BD4F9] (ab110273) was first used in a scientific publication in 2011 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-ATP5A [7H10BD4F9] (ab110273) has over 5 independent reviews from customers.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Near homogeneity as judged by SDS-PAGE. ab110273 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5A also known as ATP synthase F1 subunit alpha is a protein important for cellular energy production. As part of the ATP synthase complex it plays a mechanical role in synthesizing ATP from ADP and inorganic phosphate. The ATP5A protein has a molecular weight of approximately 55 kDa and is widely expressed in the inner mitochondrial membrane across different cell types. Its central function lies in its ability to harness the energy of the proton gradient generated by the electron transport chain to catalyze ATP synthesis.

Biological function summary

ATP5A is essential in cellular respiration serving as a catalytic core of the F1 component of ATP synthase. As part of the multi-subunit enzyme complex ATP synthase is responsible for ATP production the primary energy currency in cells. The ATP5A subunit works in conjunction with other subunits of the enzyme oligomer to facilitate the conversion of energy released during oxidative phosphorylation into a usable form. The protein's efficiency in this biological role underpins its importance in sustaining cellular energy homeostasis.

Pathways

ATP5A plays a pivotal role in oxidative phosphorylation and the electron transport chain integral components of cellular respiration. The oxidative phosphorylation pathway depends on this protein to manage the synthesis of ATP molecules while the electron transport chain creates the proton gradient necessary for ATP production. ATP5A is functionally connected to other proteins in these pathways such as ATP5B and cytochrome c oxidase working in a coordinated manner to ensure efficient energy transfer and maintenance.

ATP5A is implicative in mitochondrial disorders and neurodegenerative diseases such as Leigh syndrome and Parkinson's disease. These conditions often arise from deficits in ATP production where ineffective ATP synthase activity can contribute to cellular energy failures. In the context of Parkinson’s disease for instance ATP5A interactions with other proteins like Parkin can contribute to mitochondrial dysfunction an important pathological feature of the disorder. Through such associations alterations in ATP5A activity can significantly impact disease progression and symptomatology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit alpha, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the catalytic subunit beta (ATP5F1B), forms the catalytic core in the F(1) domain (PubMed : 37244256). Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (Probable). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (PubMed : 30146159).

See full target information ATP5F1A

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Publications (84)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 85:103680 PubMed40472774

2025

Redox signaling-mediated S-glutathionylation of protein disulfide isomerase A1 initiates intrinsic apoptosis and contributes to accelerated aging.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Wei Ye,Jie Zhang,Amit Kumar,Xuejian Huang,Theodore L Mathuram,Andrew D Mccall,John Culpepper,Leilei Zhang,Anthony D Curione,Jianqiang Xu,Kenneth D Tew,Danyelle M Townsend,Anna Blumental-Perry

iScience 27:109808 PubMed38741710

2024

Mitochondrial F0F1-ATP synthase governs the induction of mitochondrial fission.

Applications

Unspecified application

Species

Unspecified reactive species

Charlène Lhuissier,Valérie Desquiret-Dumas,Anaïs Girona,Jennifer Alban,Justine Faure,Julien Cassereau,Philippe Codron,Guy Lenaers,Olivier R Baris,Naïg Gueguen,Arnaud Chevrollier

JCI insight 9: PubMed38386420

2024

Enhancing CAR-T cell metabolism to overcome hypoxic conditions in the brain tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Ryusuke Hatae,Keith Kyewalabye,Akane Yamamichi,Tiffany Chen,Su Phyu,Pavlina Chuntova,Takahide Nejo,Lauren S Levine,Matthew H Spitzer,Hideho Okada

International journal of molecular sciences 24: PubMed37894873

2023

Characterizing Early Cardiac Metabolic Programming via 30% Maternal Nutrient Reduction during Fetal Development in a Non-Human Primate Model.

Applications

Unspecified application

Species

Unspecified reactive species

Susana P Pereira,Mariana S Diniz,Ludgero C Tavares,Teresa Cunha-Oliveira,Cun Li,Laura A Cox,Mark J Nijland,Peter W Nathanielsz,Paulo J Oliveira

International journal of molecular sciences 24: PubMed37762163

2023

Human Prune Regulates the Metabolism of Mammalian Inorganic Polyphosphate and Bioenergetics.

Applications

Unspecified application

Species

Unspecified reactive species

Ernest R Scoma,Renata T Da Costa,Ho Hang Leung,Pedro Urquiza,Mariona Guitart-Mampel,Vedangi Hambardikar,Lindsey M Riggs,Ching-On Wong,Maria E Solesio

Orphanet journal of rare diseases 18:92 PubMed37095554

2023

ATAD3A-related pontocerebellar hypoplasia: new patients and insights into phenotypic variability.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Skopkova,Hana Stufkova,Vibhuti Rambani,Viktor Stranecky,Katarina Brennerova,Miriam Kolnikova,Michaela Pietrzykova,Miloslav Karhanek,Lenka Noskova,Marketa Tesarova,Hana Hansikova,Daniela Gasperikova

Journal of extracellular vesicles 11:e12280 PubMed36382606

2022

A human kidney and liver organoid-based multi-organ-on-a-chip model to study the therapeutic effects and biodistribution of mesenchymal stromal cell-derived extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Vivian V T Nguyen,Shicheng Ye,Vasiliki Gkouzioti,Monique E van Wolferen,Fjodor Yousef Yengej,Dennis Melkert,Sofia Siti,Bart de Jong,Paul J Besseling,Bart Spee,Luc J W van der Laan,Reyk Horland,Marianne C Verhaar,Bas W M van Balkom

British journal of pharmacology 179:2678-2696 PubMed34862596

2022

Ruthenium 360 and mitoxantrone inhibit mitochondrial calcium uniporter channel to prevent liver steatosis induced by high-fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwang Zhang,Zupeng Luo,Lin Yu,Yang Xiao,Siqi Liu,Zhier ALuo,Zeqiang Ma,Liang Huang,Lianggui Xiao,Mengting Jia,Ziyi Song,Haojie Zhang,Yixing Li,Lei Zhou

Journal of animal science and biotechnology 12:94 PubMed34503581

2021

Dietary calcium supplementation promotes the accumulation of intramuscular fat.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwang Zhang,Tingli Pan,Yu Sun,Siqi Liu,Ziyi Song,Haojie Zhang,Yixing Li,Lei Zhou

Methods in molecular biology (Clifton, N.J.) 2192:287-311 PubMed33230780

2020

Blue-Native Electrophoresis to Study the OXPHOS Complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Fernandez-Vizarra,Massimo Zeviani

View all publications

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Product promise

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Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker

Western blot - Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker (AB110273)

Flow Cytometry - Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker (AB110273)

Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [7H10BD4F9] - Mitochondrial Marker (AB110273)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (84)

Product promise