Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (AB231692)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATP5A using ab176569 (unpurified) at a 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176569).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (AB231692)

ab176569 (purified) staining ATP5A in HeLa (human cervix adenocarcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 4% paraformaldehyde and permeabilized n 0.1% TritonX-100. Samples were incubated with primary antibody at 1/500 dilution (4.2µg/ml).  An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1/1000 dilution (2µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HeLa cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176569).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (AB231692)

Immunohistochemical analysis of paraffin-embedded Human fetal heart tissue labeling ATP5A using ab176569 (unpurified) at a 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176569).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (AB231692)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ATP5A with Purified ab176569 at 1 : 500 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176569).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (AB231692)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ATP5A with purified ab176569 at 1/60 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176569).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (AB231692)

Immunofluorescence analysis of MCF7 cells labeling ATP5A using ab176569 (unpurified) at a 1/100 dilution (green). DAPI nuclear staining (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176569).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (AB231692)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling ATP5A using ab176569 (unpurified) at a 1/10 dilution (red) or a rabbit IgG negative control (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176569).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (AB231692)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling ATP5A with Purified ab176569 at 1 : 500 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176569).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (AB231692)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling ATP5A with Purified ab176569 at 1 : 500 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176569).