Anti-ATP5A antibody [EPR13030(B)] (ab176569) is a rabbit monoclonal antibody that is used to detect ATP5A in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 30 publications
Images
![Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--western-blot-img125074.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)")
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunocytochemistry-immunofluorescence-img41475.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56588.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126231.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125474.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)")
Publications
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- Scientific reports 13:209482023Proteomic analyses identify HK1 and ATP5A to be overexpressed in distant metastases of lung adenocarcinomas compared to matched primary tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelen Pasternack,Mirjam Polzer,Timo Gemoll,Christiane Kümpers,Thorben Sauer,Pamela Lazar-Karsten,Sofie Hinrichs,Sabine Bohnet,Sven Perner,Franz Friedrich Dressler,Jutta KirfelPubMed 38016997
- Science advances 9:eadf26952023A conditional knockout rat resource of mitochondrial protein-coding genes via a DdCBE-induced premature stop codon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Tan,Xiaolong Qi,Weining Kong,Jiachuan Jin,Dan Lu,Xu Zhang,Yue Wang,Siting Wang,Wei Dong,Xudong Shi,Wei Chen,Jianying Wang,Keru Li,Yuan Xie,Lijuan Gao,Feifei Guan,Kai Gao,Chaojun Li,Cheng Wang,Zhibin Hu,Lianfeng Zhang,Xuejiang Guo,Bin Shen,Yuanwu MaPubMed 37058569
- Molecular therapy. Nucleic acids 32:1-122023Expanding DdCBE-mediated targeting scope to aC motif preference in rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolong Qi,Lei Tan,Xu Zhang,Jiachuan Jin,Weining Kong,Wei Chen,Jianying Wang,Wei Dong,Lijuan Gao,Lijun Luo,Dan Lu,Jianan Gong,Feifei Guan,Wenjie Shu,Xingxu Huang,Lianfeng Zhang,Shengqi Wang,Bin Shen,Yuanwu MaPubMed 36942261
- Cell death discovery 9:592023NMDARs antagonist MK801 suppresses LPS-induced apoptosis and mitochondrial dysfunction by regulating subunits of NMDARs via the CaM/CaMKII/ERK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Min Han,Xiao-Bin Hao,Yi-Xiang Hong,Shan-Shan Zhao,Xu-Chang Chen,Ruiying Wang,Yan Wang,Gang LiPubMed 36774369
- Nature communications 14:3122023A FRET-based respirasome assembly screen identifies spleen tyrosine kinase as a target to improve muscle mitochondrial respiration and exercise performance in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmi Kobayashi,Kotaro Azuma,Toshihiko Takeiwa,Toshimori Kitami,Kuniko Horie,Kazuhiro Ikeda,Satoshi InouePubMed 36697396
- Experimental & molecular medicine 55:253-2682023SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianxin Mao,Di Wang,Dong Wang,Qi Wu,Qiliang Shang,Chu Gao,Huanbo Wang,Han Wang,Mu Du,Pandi Peng,Haoruo Jia,Xiaolong Xu,Jie Wang,Liu Yang,Zhuojing LuoPubMed 36653443
- Frontiers in physiology 13:9895472022Reduced PGC-1β protein expression may underlie corticosterone inhibition of mitochondrial biogenesis and oxidative phosphorylation in chicken muscles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng Li,Zhi Wang,Jing Wen Yao,Hong Chao Jiao,Xiao Juan Wang,Hai Lin,Jing Peng ZhaoPubMed 36311241
- Frontiers in pharmacology 13:9914212022Photobiomodulation promotes repair following spinal cord injury by restoring neuronal mitochondrial bioenergetics AMPK/PGC-1α/TFAM pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhijie Zhu,Xuankang Wang,Zhiwen Song,Xiaoshuang Zuo,Yangguang Ma,Zhihao Zhang,Cheng Ju,Zhuowen Liang,Kun Li,Xueyu Hu,Zhe WangPubMed 36172183
- Cells 11:2022Beta-Amyloid Instigates Dysfunction of Mitochondria in Cardiac Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSehwan Jang,Xavier R Chapa-Dubocq,Rebecca M Parodi-Rullán,Silvia Fossati,Sabzali JavadovPubMed 35159183
- Cell death & disease 12:8392021CISD3 inhibition drives cystine-deprivation induced ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanchun Li,Xin Wang,Zhihui Huang,Yi Zhou,Jun Xia,Wanye Hu,Xu Wang,Jing Du,Xiangmin Tong,Ying WangPubMed 34497268
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected | Expected |
| Rat | Tested | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes For unpurified use at 1/50 - 1/100. The use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes For unpurified use at 1/50 - 1/100. The use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500 | Notes For unpurified use at 1/50 - 1/100. The use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (PubMed:30146159).
Alternative names
ATP5A, ATP5A1, ATP5AL2, ATPM, ATP5F1A, ATP synthase F1 subunit alpha
Recommended products
Anti-ATP5A antibody [EPR13030(B)] (ab176569) is a rabbit monoclonal antibody that is used to detect ATP5A in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 30 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13030(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-ATP5A antibody [EPR13030(B)] (ab176569) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of ATP5A?
Anti-ATP5A [EPR13030(B)] (ab176569) specifically detects a band for ATP5A (UniProt: P25705) at a molecular weight of 60kDa.
Trusted by the scientific community
Anti-ATP5A [EPR13030(B)] (ab176569) was first used in a scientific publication in 2013 and has been cited over 30 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP5A also known as ATP synthase F1 subunit alpha is a protein important for cellular energy production. As part of the ATP synthase complex it plays a mechanical role in synthesizing ATP from ADP and inorganic phosphate. The ATP5A protein has a molecular weight of approximately 55 kDa and is widely expressed in the inner mitochondrial membrane across different cell types. Its central function lies in its ability to harness the energy of the proton gradient generated by the electron transport chain to catalyze ATP synthesis.
- Biological function summary
ATP5A is essential in cellular respiration serving as a catalytic core of the F1 component of ATP synthase. As part of the multi-subunit enzyme complex ATP synthase is responsible for ATP production the primary energy currency in cells. The ATP5A subunit works in conjunction with other subunits of the enzyme oligomer to facilitate the conversion of energy released during oxidative phosphorylation into a usable form. The protein's efficiency in this biological role underpins its importance in sustaining cellular energy homeostasis.
- Pathways
ATP5A plays a pivotal role in oxidative phosphorylation and the electron transport chain integral components of cellular respiration. The oxidative phosphorylation pathway depends on this protein to manage the synthesis of ATP molecules while the electron transport chain creates the proton gradient necessary for ATP production. ATP5A is functionally connected to other proteins in these pathways such as ATP5B and cytochrome c oxidase working in a coordinated manner to ensure efficient energy transfer and maintenance.
- Associated diseases and disorders
ATP5A is implicative in mitochondrial disorders and neurodegenerative diseases such as Leigh syndrome and Parkinson's disease. These conditions often arise from deficits in ATP production where ineffective ATP synthase activity can contribute to cellular energy failures. In the context of Parkinson’s disease for instance ATP5A interactions with other proteins like Parkin can contribute to mitochondrial dysfunction an important pathological feature of the disorder. Through such associations alterations in ATP5A activity can significantly impact disease progression and symptomatology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
11 product images
![Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--western-blot-img125074.jpg "Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Western blot staining using rabbit Anti-ATP5A antibody
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569) at 0.01 µg/mL
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: Mouse brain lysates at 15 µg
Lane 3: Rat brain lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunocytochemistry-immunofluorescence-img41475.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-ATP5A antibody
ab176569 (purified) staining ATP5A in HeLa (human cervix adenocarcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 4% paraformaldehyde and permeabilized n 0.1% TritonX-100. Samples were incubated with primary antibody at 1/500 dilution (4.2μg/ml). An AlexaFluor®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as a secondary antibody at 1/1000 dilution (2μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HeLa cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56588.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ATP5A antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling ATP5A with Purified ab176569 at 1:500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126231.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ATP5A antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling ATP5A with Purified ab176569 at 1:500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125474.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ATP5A antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ATP5A with Purified ab176569 at 1:500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
![Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--flow-cytometry-intracellular-img32522.jpg "Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Flow Cytometry (Intracellular) staining using rabbit Anti-ATP5A antibody
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ATP5A with purified ab176569 at 1/60 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--western-blot-img103671.jpg "Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Western blot staining using rabbit Anti-ATP5A antibody
All lanes: Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: Human fetal liver lysate at 10 µg
Lane 4: Human fetal lung lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunocytochemistry-immunofluorescence-img26910.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-ATP5A antibody
Immunofluorescence analysis of MCF7 cells labeling ATP5A using ab176569 (unpurified) at a 1/100 dilution (green). DAPI nuclear staining (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105808.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ATP5A antibody
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATP5A using ab176569 (unpurified) at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125424.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ATP5A antibody
Immunohistochemical analysis of paraffin-embedded Human fetal heart tissue labeling ATP5A using ab176569 (unpurified) at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--flow-cytometry-intracellular-img27334.jpg "Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)
ATP5A Flow Cytometry (Intracellular) staining using rabbit Anti-ATP5A antibody
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling ATP5A using ab176569 (unpurified) at a 1/10 dilution (red) or a rabbit IgG negative control (green).
![Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--flow-cytometry-intracellular-img32522.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
![Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569), expandable thumbnail](https://content.abcam.com/products/images/atp5a-antibody-epr13030b-mitochondrial-marker-ab176569--western-blot-img103671.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569)")
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