Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling ATP5A using ab176569 (unpurified) at a 1/10 dilution (red) or a rabbit IgG negative control (green).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATP5A using ab176569 (unpurified) at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

Immunohistochemical analysis of paraffin-embedded Human fetal heart tissue labeling ATP5A using ab176569 (unpurified) at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ATP5A with Purified ab176569 at 1 : 500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ATP5A with purified ab176569 at 1/60 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

Immunofluorescence analysis of MCF7 cells labeling ATP5A using ab176569 (unpurified) at a 1/100 dilution (green). DAPI nuclear staining (blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

ab176569 (purified) staining ATP5A in HeLa (human cervix adenocarcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 4% paraformaldehyde and permeabilized n 0.1% TritonX-100. Samples were incubated with primary antibody at 1/500 dilution (4.2μg/ml). An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1/1000 dilution (2μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HeLa cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling ATP5A with Purified ab176569 at 1 : 500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling ATP5A with Purified ab176569 at 1 : 500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

• WB

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Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569) at 0.01 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Mouse brain lysates at 15 µg

Lane 3:

Rat brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 60 kDa

false

• WB

Supplier Data

Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (AB176569)

All lanes:

Western blot - Anti-ATP5A antibody [EPR13030(B)] - Mitochondrial Marker (ab176569) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Lane 4:

Human fetal lung lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 60 kDa

true