AB188107

Anti-ATP5A antibody - Mitochondrial Marker

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(3 Publications)

Goat Polyclonal ATP5A antibody. Suitable for WB and reacts with Mouse, Rat, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ATP5F1A aa 50-100.

View Alternative Names

ATP5A, ATP5A1, ATP5AL2, ATPM, ATP5F1A, ATP synthase F1 subunit alpha

2 Images

Supplier Data

Western blot - Anti-ATP5A antibody - Mitochondrial Marker (AB188107)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ATP5A antibody - Mitochondrial Marker (ab188107) at 0.01 µg/mL

Lane 1:

Human heart lysate (in RIPA buffer) at 35 µg

Lane 2:

Fetal mouse heart lysate (in RIPA buffer) at 35 µg

Lane 3:

Adult mouse heart lysate (in RIPA buffer) at 35 µg

Lane 4:

Adult rat heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 60 kDa

Observed band size: 52 kDa

true

Supplier Data

Western blot - Anti-ATP5A antibody - Mitochondrial Marker (AB188107)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ATP5A antibody - Mitochondrial Marker (ab188107) at 0.01 µg/mL

All lanes:

Pig heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 60 kDa

Observed band size: 55 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

applications

Immunogen

Synthetic Peptide within Human ATP5F1A aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P25705

Specificity

ab188107 is expected to recognize all reported isoforms (NP_004037.1; NP_001244263.1; NP_001001935.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab188107 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffer, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5A also known as ATP synthase F1 subunit alpha is a protein important for cellular energy production. As part of the ATP synthase complex it plays a mechanical role in synthesizing ATP from ADP and inorganic phosphate. The ATP5A protein has a molecular weight of approximately 55 kDa and is widely expressed in the inner mitochondrial membrane across different cell types. Its central function lies in its ability to harness the energy of the proton gradient generated by the electron transport chain to catalyze ATP synthesis.

Biological function summary

ATP5A is essential in cellular respiration serving as a catalytic core of the F1 component of ATP synthase. As part of the multi-subunit enzyme complex ATP synthase is responsible for ATP production the primary energy currency in cells. The ATP5A subunit works in conjunction with other subunits of the enzyme oligomer to facilitate the conversion of energy released during oxidative phosphorylation into a usable form. The protein's efficiency in this biological role underpins its importance in sustaining cellular energy homeostasis.

Pathways

ATP5A plays a pivotal role in oxidative phosphorylation and the electron transport chain integral components of cellular respiration. The oxidative phosphorylation pathway depends on this protein to manage the synthesis of ATP molecules while the electron transport chain creates the proton gradient necessary for ATP production. ATP5A is functionally connected to other proteins in these pathways such as ATP5B and cytochrome c oxidase working in a coordinated manner to ensure efficient energy transfer and maintenance.

ATP5A is implicative in mitochondrial disorders and neurodegenerative diseases such as Leigh syndrome and Parkinson's disease. These conditions often arise from deficits in ATP production where ineffective ATP synthase activity can contribute to cellular energy failures. In the context of Parkinson’s disease for instance ATP5A interactions with other proteins like Parkin can contribute to mitochondrial dysfunction an important pathological feature of the disorder. Through such associations alterations in ATP5A activity can significantly impact disease progression and symptomatology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions (PubMed : 30146159).

See full target information ATP5F1A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 49:4689-4704 PubMed33836087

2021

Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghai Zhang,Xiao He,Shihao Yao,Tianxiang Lin,Luwen Zhang,Danni Chen,Chao Chen,Qingxian Yang,Feng Li,Yi-Min Zhu,Min-Xin Guan

Cell 175:1088-1104.e23 PubMed30318146

2018

The 7q11.23 Protein DNAJC30 Interacts with ATP Synthase and Links Mitochondria to Brain Development.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew T N Tebbenkamp,Luis Varela,Jinmyung Choi,Miguel I Paredes,Alice M Giani,Jae Eun Song,Matija Sestan-Pesa,Daniel Franjic,André M M Sousa,Zhong-Wu Liu,Mingfeng Li,Candace Bichsel,Marco Koch,Klara Szigeti-Buck,Fuchen Liu,Zhuo Li,Yuka I Kawasawa,Constantinos D Paspalas,Yann S Mineur,Paolo Prontera,Giuseppe Merla,Marina R Picciotto,Amy F T Arnsten,Tamas L Horvath,Nenad Sestan

Nucleic acids research 46:10930-10945 PubMed30137487

2018

Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghai Zhang,Luwen Zhang,Danni Chen,Xiao He,Shihao Yao,Zengming Zhang,Ye Chen,Min-Xin Guan

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ATP5A antibody - Mitochondrial Marker

Western blot - Anti-ATP5A antibody - Mitochondrial Marker (AB188107)

Western blot - Anti-ATP5A antibody - Mitochondrial Marker (AB188107)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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