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AB119686

Anti-ATP5C1 antibody [2A1AA11]

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(7 Publications)

Mouse Monoclonal ATP5C1 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat, Cow samples. Cited in 7 publications.

View Alternative Names

ATP5C, ATP5C1, ATP5CL1, ATP5F1C, ATP synthase F1 subunit gamma, F-ATPase gamma subunit

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5C1 antibody [2A1AA11] (AB119686)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5C1 antibody [2A1AA11] (AB119686)

IHC image of ATP5C1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119686, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-ATP5C1 antibody [2A1AA11] (AB119686)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATP5C1 antibody [2A1AA11] (AB119686)

Immunocytochemistry using ab119686 stained HDFn cells (human). The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with antigen retrieval. The cells were then incubated with the antibody (ab119686, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.

Flow Cytometry - Anti-ATP5C1 antibody [2A1AA11] (AB119686)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-ATP5C1 antibody [2A1AA11] (AB119686)

Flow cytometry. Hela cells were stained with 1μg/mL anti-ATP5C1 antibody (ab119686) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Western blot - Anti-ATP5C1 antibody [2A1AA11] (AB119686)
  • WB

Unknown

Western blot - Anti-ATP5C1 antibody [2A1AA11] (AB119686)

All lanes:

Western blot - Anti-ATP5C1 antibody [2A1AA11] (ab119686) at 1 µg/mL

Lane 1:

human heart homogenate lysate at 15 µg

Lane 2:

human HepG2 cell lysate at 15 µg

Lane 3:

human liver mitochondria lysate at 7.5 µg

Lane 4:

rat liver mitochondria lysate at 7.5 µg

Lane 5:

mouse liver mitochondria lysate at 7.5 µg

Lane 6:

bovine heart mitochondria lysate at 7.5 µg

Secondary

All lanes:

Goat anti-mouse HRP at 1/5000 dilution

Predicted band size: 33 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2A1AA11

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Cow, Human

Applications

IHC-P, WB, ICC/IF, Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Precipitation Ammonium Sulphate
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ATP5C1 protein also known as ATP synthase subunit gamma plays an important role in cellular energy production. This protein has a mass of approximately 55 kDa. It remains primarily expressed in the mitochondria the powerhouse of cells where it forms a part of the ATP synthase complex. ATP5C1 is essential for ATP synthesis a pivotal process for energy production in cells.
Biological function summary

The ATP5C1 protein is an integral component of the F1 sector of the ATP synthase complex. This complex transduces electrochemical energy into ATP which is the main energy carrier in biological systems. ATP5C1 facilitates rotation of the complex’s central stalk enabling energy conversion. It ensures ATP is produced efficiently supporting various cellular processes. ATP5C1 expression levels affect cellular metabolism and energy homeostasis.

Pathways

ATP5C1 holds an important position within oxidative phosphorylation and the mitochondrial electron transport chain. These pathways are vital for ATP production and involve several proteins and enzymes including NADH dehydrogenase cytochrome c oxidase and other ATP synthase subunits. ATP5C1 interacts with these proteins to sustain the flow of electrons coupling it with proton gradients that drive ATP synthesis.

ATP5C1 mutations or malfunctions link to conditions such as mitochondrial diseases and metabolic disorders. For instance alterations in ATP5C1 can affect ATP synthesis efficiency leading to mitochondrial dysfunction. It also associates with neurodegenerative diseases where inefficient energy production exacerbates neuronal degradation. The protein's dysfunction may involve other proteins like ATP5D a fellow subunit within the ATP synthase complex that collaborates in maintaining ATP production.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit gamma, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). With the central stalk subunit delta, is essential for the biogenesis of F(1) catalytic part of the ATP synthase complex namely in the formation of F1 assembly intermediate (PubMed : 29499186).
See full target information ATP5F1C
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes research 2020:2057187 PubMed32083134

2020

Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNA Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Li,Lijun Wu,Jianjiang Liu,Wei Lin,Qiang Qi,Tianlan Zhao

Cell metabolism 27:1026-1039.e6 PubMed29606596

2018

Metabolic Maturation during Muscle Stem Cell Differentiation Is Achieved by miR-1/133a-Mediated Inhibition of the Dlk1-Dio3 Mega Gene Cluster.

Applications

Unspecified application

Species

Unspecified reactive species

Stas Wüst,Stefan Dröse,Juliana Heidler,Ilka Wittig,Ina Klockner,Andras Franko,Erik Bonke,Stefan Günther,Ulrich Gärtner,Thomas Boettger,Thomas Braun

The Biochemical journal 429:205-13 PubMed20415663

2010

Pyruvate dehydrogenase kinase 1 controls mitochondrial metabolism and insulin secretion in INS-1 832/13 clonal beta-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrika Krus,Olga Kotova,Peter Spégel,Elna Hallgard,Vladimir V Sharoyko,Anna Vedin,Thomas Moritz,Mary C Sugden,Thomas Koeck,Hindrik Mulder

Molecular and cellular biology 30:1303-18 PubMed20100866

2010

Mitochondrial p32 protein is a critical regulator of tumor metabolism via maintenance of oxidative phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Fogal,Adam D Richardson,Priya P Karmali,Immo E Scheffler,Jeffrey W Smith,Erkki Ruoslahti

Journal of emergencies, trauma, and shock 2:67-72 PubMed19561963

2009

Role of pyruvate dehydrogenase complex in traumatic brain injury and Measurement of pyruvate dehydrogenase enzyme by dipstick test.

Applications

Unspecified application

Species

Unspecified reactive species

Pushpa Sharma,Brandi Benford,Zhao Z Li,Geoffrey Sf Ling

Endocrinology 150:4104-13 PubMed19556420

2009

Activation of liver X receptor regulates substrate oxidation in white adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Britta M Stenson,Mikael Rydén,Knut R Steffensen,Kerstin Wåhlén,Amanda T Pettersson,Johan W Jocken,Peter Arner,Jurga Laurencikiene

FASEB journal : official publication of the Federa 22:3358-69 PubMed18511551

2008

Human tumor nanoparticles induce apoptosis of pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elodie Ristorcelli,Evelyne Beraud,Patrick Verrando,Claude Villard,Daniel Lafitte,Véronique Sbarra,Dominique Lombardo,Alain Verine
View all publications
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Product promise

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