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AB174438

Anti-ATP5D antibody [4D11]

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(2 Publications)

Mouse Monoclonal ATP5D antibody. Suitable for WB and reacts with Mouse, Rat, Cow samples. Cited in 2 publications.

View Alternative Names

ATP5D, ATP synthase F1 subunit delta, F-ATPase delta subunit

1 Images
Western blot - Anti-ATP5D antibody [4D11] (AB174438)
  • WB

Supplier Data

Western blot - Anti-ATP5D antibody [4D11] (AB174438)

All lanes:

Western blot - Anti-ATP5D antibody [4D11] (ab174438) at 1 µg/mL

Lane 1:

Human Heart Homogenate at 10 µg

Lane 2:

Human Liver Homogenate at 10 µg

Lane 3:

Rat Liver Homogenate at 10 µg

Lane 4:

Mouse Liver Homogenate at 10 µg

Lane 5:

Bovine Heart Homogenate at 10 µg

Lane 6:

Human HepG2 cell lysate at 20 µg

Lane 7:

Rat H4IIE cell lysate at 20 µg

Lane 8:

Mouse MEF cell lysate at 20 µg

Secondary

All lanes:

Goat anti Mouse IR700 in 5% milk/PBST at 1/5000 dilution

Predicted band size: 17 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4D11

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Cow

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification notes
ab174438 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99.9% HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5D also known as ATP synthase subunit delta is a part of the mitochondrial ATP synthase complex. This protein plays a role in the conversion of ADP to ATP by facilitating proton flow across mitochondrial membranes. ATP5D has a molecular mass of approximately 18-20 kDa. Expression of ATP5D occurs in tissues with high energy demands such as the heart skeletal muscle and liver reflecting its role in energy metabolism.
Biological function summary

ATP5D contributes to the production of cellular energy by acting as an essential subunit in the F1 region of the F1Fo ATP synthase complex. This complex is responsible for ATP synthesis during oxidative phosphorylation. ATP5D's role is critical for maintaining the structural integrity and function of the ATP synthase complex. Its presence ensures efficient ATP production essential for various cellular processes.

Pathways

ATP5D functions within oxidative phosphorylation and energy metabolism pathways. It is closely associated with other ATP synthase subunits required for the ATP production process. Complex interactions include interactions with proteins such as ATP5A and ATP5B within the ATP synthase complex which work together in the energy production pathway essential for cell survival.

Defects or mutations in ATP5D can be related to mitochondrial diseases like mitochondrial encephalomyopathy. Alterations in its normal function can lead to improper cellular energy production. Additionally ATP5D mutations may impact disorders like Leigh syndrome where mitochondrial dysfunction plays a critical role. Understanding ATP5D's connections including those with proteins involved in these disorders helps unravel its impact on these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
See full target information ATP5F1D
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:67 PubMed40266594

2025

Microglia-Derived IL-6 Promotes Müller Glia Reprogramming and Proliferation in Zebrafish Retina Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xu,Yi Li,Xiangyu Li,Xuan Tan,Lihua Liu,Lining Cao,Hui Xu

The EMBO journal 43:6001-6031 PubMed39424953

2024

The mitochondrial long non-coding RNA lncMtloop regulates mitochondrial transcription and suppresses Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wandi Xiong,Kaiyu Xu,Jacquelyne Ka-Li Sun,Siling Liu,Baizhen Zhao,Jie Shi,Karl Herrup,Hei-Man Chow,Lin Lu,Jiali Li
View all publications
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