AB117991

Anti-ATP5F1 antibody [9D1BC4]

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(15 Publications)

Mouse Monoclonal ATP5F1 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Cow, Rat samples. Cited in 15 publications.

View Alternative Names

ATP5F1, ATP5PB, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATPase subunit b

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

IHC image of ATP5F1 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117991, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow Cytometry - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

Flow Cyt

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Flow Cytometry - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

ab117991 at 1 ug/ml with Hela cells in flow cytometry.

Immunocytochemistry/ Immunofluorescence - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

Immunocytochemistry image of ab117991 (MS972) stained NIH3T3 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with urea/heat antigen retrieval method. The cells were incubated with ab117991 at 1 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates to the mitochondria. The four cells in the upper portion of the image show mitochondria in the elongated, reticular, arrangement, the three cells in the lower portion of the image show a punctuate mitochondrial organization and may be dividing/have recently divided.

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Western blot - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

Note - Extra bands seen in lane 6 are secondary antibody mouse-on-mouse effects and not related to the primary antibody.

All lanes:

Western blot - Anti-ATP5F1 antibody [9D1BC4] (ab117991) at 1 µg/mL

Lane 1:

Human heart homogenate at 15 µg

Lane 2:

HepG2 lysate at 15 µg

Lane 3:

Human liver mitochondria at 7.5 µg

Lane 4:

Bovine heart mitochondria at 7.5 µg

Lane 5:

Rat liver mitochondria at 7.5 µg

Lane 6:

Mouse liver mitochondria at 7.5 µg

Predicted band size: 29 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9D1BC4

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

IHC-P, WB, ICC/IF, Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5F1 also known as ATP synthase F(0) complex subunit B1 is a protein found in the inner mitochondrial membrane. It is a component of the ATP synthase enzyme an important player in energy production with a molecular weight around 55 kDa. ATP5F1 is expressed abundantly in energy-demanding tissues such as the heart liver and skeletal muscle. This protein plays an important mechanical role in synthesizing adenosine triphosphate (ATP) by catalyzing the combination of adenosine diphosphate (ADP) and inorganic phosphate.

Biological function summary

ATP5F1 serves as an important part of the ATP synthase complex which consists of various subunits forming the F1 and Fo regions. This complex carries out the final step of oxidative phosphorylation in the mitochondria by using the proton gradient across the mitochondrial membrane to drive ATP production. As a critical process in cellular respiration ATP generation influences numerous cellular functions including muscle contraction nerve impulse propagation and synthesis of biomolecules.

Pathways

ATP5F1 operates within the oxidative phosphorylation and electron transport chain pathways. These pathways are important for maintaining the cell's energy balance. ATP5F1 works closely with other proteins such as ATP synthase Fo subunits aiding in proton translocation and ensuring efficient ATP synthesis. Disruptions in these pathways can have significant effects on cellular metabolism and energy homeostasis.

ATP5F1 is linked to mitochondrial dysfunctions such as mitochondrial myopathy and Leigh syndrome. These conditions result from impaired oxidative phosphorylation leading to insufficient ATP production. ATP5F1 interacts with the NADH dehydrogenase complex and mutations can affect their functionality contributing to these disorders. Understanding ATP5F1's role helps researchers explore therapeutic strategies aimed at managing these mitochondrial diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

See full target information ATP5PB

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70609 PubMed40525396

2025

LSMEM2, Localized at the Neuromuscular Junction, Modulates Mitochondrial Integration in Skeletal Muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Eman Elrefaei,Satoru Yamazaki,Issei Yazawa,Yusuke Takahashi,Naoki Ito,Nozomi Hayashiji,Yuya Nishida,Ichizo Nishino,Seiji Takashima,Yasunori Shintani

Science advances 11:eadq1575 PubMed40184463

2025

Atf3 controls transitioning in female mitochondrial cardiomyopathy as identified by spatial and single-cell transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Tasneem Qaqorh,Yusuke Takahashi,Kohei Sameshima,Kentaro Otani,Issei Yazawa,Yuya Nishida,Kohei Tonai,Yoshitaka Fujihara,Mizuki Honda,Shinya Oki,Yasuyuki Ohkawa,David R Thorburn,Ann E Frazier,Atsuhito Takeda,Yoshihiko Ikeda,Heima Sakaguchi,Takuya Watanabe,Norihide Fukushima,Yasumasa Tsukamoto,Naomasa Makita,Osamu Yamaguchi,Kei Murayama,Akira Ohtake,Yasushi Okazaki,Takanari Kimura,Hisakazu Kato,Hijiri Inoue,Ken Matsuoka,Seiji Takashima,Yasunori Shintani

Nature communications 14:2628 PubMed37149717

2023

Mapping PTBP2 binding in human brain identifies SYNGAP1 as a target for therapeutic splice switching.

Applications

Unspecified application

Species

Unspecified reactive species

Jennine M Dawicki-McKenna,Alex J Felix,Elisa A Waxman,Congsheng Cheng,Defne A Amado,Paul T Ranum,Alexey Bogush,Lea V Dungan,Jean Ann Maguire,Alyssa L Gagne,Elizabeth A Heller,Deborah L French,Beverly L Davidson,Benjamin L Prosser

Scientific reports 12:8758 PubMed35610292

2022

Activation of Gαq sequesters specific transcripts into Ago2 particles.

Applications

Unspecified application

Species

Unspecified reactive species

Lela Jackson,Madison Rennie,Alison Poussaint,Suzanne Scarlata

Nature communications 13:2769 PubMed35589699

2022

Mitochondrial calcium uniporter stabilization preserves energetic homeostasis during Complex I impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Enrique Balderas,David R Eberhardt,Sandra Lee,John M Pleinis,Salah Sommakia,Anthony M Balynas,Xue Yin,Mitchell C Parker,Colin T Maguire,Scott Cho,Marta W Szulik,Anna Bakhtina,Ryan D Bia,Marisa W Friederich,Timothy M Locke,Johan L K Van Hove,Stavros G Drakos,Yasemin Sancak,Martin Tristani-Firouzi,Sarah Franklin,Aylin R Rodan,Dipayan Chaudhuri

Biomedicines 10: PubMed35203486

2022

Genetic Complementation of ATP Synthase Deficiency Due to Dysfunction of TMEM70 Assembly Factor in Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandra Marković,Kateřina Tauchmannová,Miroslava Šimáková,Petr Mlejnek,Vilma Kaplanová,Petr Pecina,Alena Pecinová,František Papoušek,František Liška,Jan Šilhavý,Jana Mikešová,Jan Neckář,Josef Houštěk,Michal Pravenec,Tomáš Mráček

Particle and fibre toxicology 18:44 PubMed34911549

2021

Transcriptomics of single dose and repeated carbon black and ozone inhalation co-exposure highlight progressive pulmonary mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Quincy A Hathaway,Nairrita Majumder,William T Goldsmith,Amina Kunovac,Mark V Pinti,Jack R Harkema,Vince Castranova,John M Hollander,Salik Hussain

Nature communications 12:4835 PubMed34376679

2021

Defining the molecular mechanisms of the mitochondrial permeability transition through genetic manipulation of F-ATP synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Carrer,Ludovica Tommasin,Justina Šileikytė,Francesco Ciscato,Riccardo Filadi,Andrea Urbani,Michael Forte,Andrea Rasola,Ildikò Szabò,Michela Carraro,Paolo Bernardi

Journal of Alzheimer's disease : JAD 78:1743-1753 PubMed33285636

2020

OGA Inhibition Alters Energetics and Nutrient Sensing in Alzheimer's Disease Cytoplasmic Hybrids.

Applications

Unspecified application

Species

Unspecified reactive species

Jarrod Flax,Heather M Wilkins,Reegan Miller,Sarah Griffith,Gentry K Cork,Amy Qiang,Jeffrey Thompson,Russell H Swerdlow,Chad Slawson

Cell reports 32:108095 PubMed32877677

2020

The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore.

Applications

Unspecified application

Species

Unspecified reactive species

Michela Carraro,Kristen Jones,Geppo Sartori,Marco Schiavone,Salvatore Antonucci,Roza Kucharczyk,Jean-Paul di Rago,Cinzia Franchin,Giorgio Arrigoni,Michael Forte,Paolo Bernardi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP5F1 antibody [9D1BC4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

Flow Cytometry - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

Immunocytochemistry/ Immunofluorescence - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

Western blot - Anti-ATP5F1 antibody [9D1BC4] (AB117991)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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