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Rabbit Recombinant Monoclonal ATP synthase C antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

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Images

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149), expandable thumbnail
  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149), expandable thumbnail
  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149), expandable thumbnail
  • Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Expected
Rat
Not recommended
Tested
Expected

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/2000 - 1/10000
Notes

-

Species
Human
Dilution info
1/2000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.

Alternative names

ATP5G1, ATP5MC1, ATP synthase lipid-binding protein, ATP synthase membrane subunit c locus 1, ATP synthase proteolipid P1, ATP synthase proton-transporting mitochondrial F(0) complex subunit C1, ATPase protein 9, ATPase subunit c

Recommended products

Rabbit Recombinant Monoclonal ATP synthase C antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13908
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATP5G1 ATP5G2 and ATP5G3 also known as subunits c of the mitochondrial ATP synthase are integral components of the F0 membrane sector of ATP synthase. These proteins have a mass of approximately 8.2 kilodaltons and play an important role in the proton-conducting pore of the enzyme. Expression occurs mainly in tissues with high energy demands such as cardiac and skeletal muscles. As integral membrane proteins they anchor as oligomers in the inner mitochondrial membrane and participate in the process of ATP production.

Biological function summary

ATP5G1 ATP5G2 and ATP5G3 form part of the ATP synthase complex which is essential for oxidative phosphorylation and cellular energy production. The ATP synthase complex consists of two main parts: F1 and F0. The subunits c encoded by ATP5G1 ATP5G2 and ATP5G3 construct a rotary motor within the F0 complex that allows proton translocation across the inner mitochondrial membrane. This process facilitates the production of ATP from ADP and inorganic phosphate which is the primary energy currency of the cell.

Pathways

ATP5G1 ATP5G2 and ATP5G3 subunits participate in the oxidative phosphorylation pathway an important component of cellular respiration. This pathway plays a major role in energy generation by creating ATP through electron transport and chemiosmotic coupling mechanisms. The ATP synthase complex works in conjunction with other proteins like cytochrome c oxidase in the electron transport chain. Efficient functioning of these proteins ensures ATP production which is vital for various cellular processes.

Associated diseases and disorders

Defects or malfunctions in ATP5G1 ATP5G2 and ATP5G3 have associations with mitochondrial disorders and neuromuscular diseases. Dysfunction in the ATP synthase complex can lead to conditions such as Leigh syndrome and other mitochondrial encephalomyopathies. The subunits are also connected with proteins involved in these disorders including the adenine nucleotide translocase. Understanding these relationships helps in diagnosing and developing therapeutic strategies for these energy deficiency-related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149), expandable thumbnail

    Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution

    Lane 1: mouse heart lysate at 10 µg

    Lane 2: rat heart lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 14 kDa

    Observed band size: 8 kDa

  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149), expandable thumbnail

    Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution

    All lanes: human fetal heart tissue lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 14 kDa, 33 kDa, 48 kDa

    Observed band size: 37 kDa, 48 kDa, 8 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

    Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab180149 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149), expandable thumbnail

    Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

    All lanes: Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: Human fetal heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

    Predicted band size: 14 kDa

  • Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149), expandable thumbnail

    Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

    Western blot analysis of fetal heart lysate immunoprecipitated with unpurified ab180149 at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

    All lanes: Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

    Immuohistochemical analysis of Paraffin-embedded Human liver tissue labeling ATP5G1 with unpurified ab180149 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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