AB180149

Anti-ATP5G1/G2/G3 antibody [EPR13908]

RabMAb
Recombinant
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(22 Publications)

Rabbit Recombinant Monoclonal ATP synthase C antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 22 publications.

View Alternative Names

ATP5G1, ATP5MC1, ATP synthase lipid-binding protein, ATP synthase membrane subunit c locus 1, ATP synthase proteolipid P1, ATP synthase proton-transporting mitochondrial F(0) complex subunit C1, ATPase protein 9, ATPase subunit c

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Immuohistochemical analysis of Paraffin-embedded Human liver tissue labeling ATP5G1 with unpurified ab180149 at 1/100 dilution followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab180149 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Supplier Data

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

All lanes:

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Human fetal heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 14 kDa

false

Lab

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution

All lanes:

human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 14 kDa,33 kDa,48 kDa

Observed band size: 37 kDa,48 kDa,8 kDa

false

Lab

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution

Lane 1:

mouse heart lysate at 10 µg

Lane 2:

rat heart lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 8 kDa

false

Supplier Data

Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Western blot analysis of fetal heart lysate immunoprecipitated with unpurified ab180149 at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149)

false

Carrier free

Anti-ATP5G1/G2/G3 antibody [EPR13908] - BSA and Azide free
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-ATP5G1/G2/G3 antibody [EPR13908]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ATP5G1/G2/G3 antibody [EPR13908]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ATP5G1/G2/G3 antibody [EPR13908]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-ATP5G1/G2/G3 antibody [EPR13908]
578 PE

PE Anti-ATP5G1/G2/G3 antibody [EPR13908]
660 APC

APC Anti-ATP5G1/G2/G3 antibody [EPR13908]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-ATP5G1/G2/G3 antibody [EPR13908]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13908

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5G1 ATP5G2 and ATP5G3 also known as subunits c of the mitochondrial ATP synthase are integral components of the F0 membrane sector of ATP synthase. These proteins have a mass of approximately 8.2 kilodaltons and play an important role in the proton-conducting pore of the enzyme. Expression occurs mainly in tissues with high energy demands such as cardiac and skeletal muscles. As integral membrane proteins they anchor as oligomers in the inner mitochondrial membrane and participate in the process of ATP production.

Biological function summary

ATP5G1 ATP5G2 and ATP5G3 form part of the ATP synthase complex which is essential for oxidative phosphorylation and cellular energy production. The ATP synthase complex consists of two main parts: F1 and F0. The subunits c encoded by ATP5G1 ATP5G2 and ATP5G3 construct a rotary motor within the F0 complex that allows proton translocation across the inner mitochondrial membrane. This process facilitates the production of ATP from ADP and inorganic phosphate which is the primary energy currency of the cell.

Pathways

ATP5G1 ATP5G2 and ATP5G3 subunits participate in the oxidative phosphorylation pathway an important component of cellular respiration. This pathway plays a major role in energy generation by creating ATP through electron transport and chemiosmotic coupling mechanisms. The ATP synthase complex works in conjunction with other proteins like cytochrome c oxidase in the electron transport chain. Efficient functioning of these proteins ensures ATP production which is vital for various cellular processes.

Defects or malfunctions in ATP5G1 ATP5G2 and ATP5G3 have associations with mitochondrial disorders and neuromuscular diseases. Dysfunction in the ATP synthase complex can lead to conditions such as Leigh syndrome and other mitochondrial encephalomyopathies. The subunits are also connected with proteins involved in these disorders including the adenine nucleotide translocase. Understanding these relationships helps in diagnosing and developing therapeutic strategies for these energy deficiency-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit c, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). With the subunit a (MT-ATP6), forms the proton-conducting channel in the F(0) domain, that contains two crucial half-channels (inlet and outlet) that facilitate proton movement from the mitochondrial intermembrane space (IMS) into the matrix (PubMed : 37244256). Protons are taken up via the inlet half-channel and released through the outlet half-channel, following a Grotthuss mechanism (PubMed : 37244256).

See full target information ATP5MC1

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiovascular development and disease 12: PubMed40863384

2025

Dysfunctional Electron Transport Chain Assembly in COXPD8.

Applications

Unspecified application

Species

Unspecified reactive species

Gisela Beutner,Heidie L Huyck,Gail Deutsch,Gloria S Pryhuber,George A Porter

Nature communications 16:6861 PubMed40715150

2025

Tumour initiated purinergic signalling promotes cardiomyocyte RBFOX1 degradation and cardiotoxicity from DNA damaging anticancer agents.

Applications

Unspecified application

Species

Unspecified reactive species

Saymon Tejay,Maria Areli Lorenzana-Carrillo,Guocheng Huang,Seyed Amirhossein Tabatabaei Dakhili,Yuan -Yuan Zhao,Farah Eaton,Michelle Mendiola Pla,Dawn E Bowles,Adam Kinnaird,D Ian Paterson,Edith Pituskin,John R Ussher,Evangelos D Michelakis,Gopinath Sutendra

iScience 28:112296 PubMed40276776

2025

Metabolic alterations and immune heterogeneity in gastric cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zeng,Zhihao Lin,Feiyan Feng,Yanyan Li,Weiwei Liu,Wenting He,Yongjun Huang,Xingtao Lin,Yan Mei,Hongmei Wu,Qingling Zhang

Cell death and differentiation 32:1518-1535 PubMed40108410

2025

Cryo-EM structure of the brine shrimp mitochondrial ATP synthase suggests an inactivation mechanism for the ATP synthase leak channel.

Applications

Unspecified application

Species

Unspecified reactive species

Amrendra Kumar,Juliana da Fonseca Rezende E Mello,Yangyu Wu,Daniel Morris,Ikram Mezghani,Erin Smith,Stephane Rombauts,Peter Bossier,Juno Krahn,Fred J Sigworth,Nelli Mnatsakanyan

Biochemical genetics 63:5394-5414 PubMed39656403

2024

Inflammatory Signaling Induces Mitochondrial Dysfunction and Neuronal Death in Traumatic Brain Injury via Downregulation of OXPHOS Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Dong,Hui Zhang,Lei Cai,Quanyi Ye,Heping Wang,Bo Liu,Wenhu Zhang,Junxin Li

Biomolecules 14: PubMed39595574

2024

Molecular Profiling of Mouse Models of Loss or Gain of Function of the KCNT1 (Slack) Potassium Channel and Antisense Oligonucleotide Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Fangxu Sun,Huafeng Wang,Jing Wu,Imran H Quraishi,Yalan Zhang,Maysam Pedram,Benbo Gao,Elizabeth A Jonas,Viet Nguyen,Sijia Wu,Omar S Mabrouk,Paymaan Jafar-Nejad,Leonard K Kaczmarek

Discover oncology 15:631 PubMed39514138

2024

Construction of a novel mitochondrial oxidative stress-related genes prognostic system and molecular subtype characterization for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Yang Li,Yanzheng Zhu,Min Wang,Zheyao Luan

iScience 27:110560 PubMed39184436

2024

Mitochondrial translation is the primary determinant of secondary mitochondrial complex I deficiencies.

Applications

Unspecified application

Species

Unspecified reactive species

Kristýna Čunátová,Marek Vrbacký,Guillermo Puertas-Frias,Lukáš Alán,Marie Vanišová,María José Saucedo-Rodríguez,Josef Houštěk,Erika Fernández-Vizarra,Jiří Neužil,Alena Pecinová,Petr Pecina,Tomáš Mráček

Genes 15: PubMed38397235

2024

Homozygous Mutation and Neurodegeneration in Weimaraners: Myelin Abnormalities and Accumulation of Lipofuscin-like Inclusions.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan H Keller,Gary S Johnson,Garrett Bullock,Tendai Mhlanga-Mutangadura,Malte Schwartz,Savannah G Pattridge,Juyuan Guo,Gregg D Kortz,Martin L Katz

Proceedings of the National Academy of Sciences of the United States of America 120:e2303713120 PubMed38091291

2023

The mitochondrial ATP synthase is a negative regulator of the mitochondrial permeability transition pore.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan Pekson,Felix G Liang,Joshua L Axelrod,Jaehoon Lee,Dongze Qin,Andre J H Wittig,Victor M Paulino,Min Zheng,Pablo M Peixoto,Richard N Kitsis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP5G1/G2/G3 antibody [EPR13908]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Western blot - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Immunoprecipitation - Anti-ATP5G1/G2/G3 antibody [EPR13908] (AB180149)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

Product promise