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AB173006

Anti-ATP5H antibody [10G5AB2]

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(1 Review)

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(2 Publications)

Mouse Monoclonal ATP5H antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat, African green monkey, Cow samples. Cited in 2 publications.

View Alternative Names

ATP5H, My032, ATP5PD, ATPase subunit d, ATP synthase peripheral stalk subunit d

4 Images
Flow Cytometry - Anti-ATP5H antibody [10G5AB2] (AB173006)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-ATP5H antibody [10G5AB2] (AB173006)

Flow Cytometry with HeLa cells stained with ab173006 (blue) and isotype-specific negative control (red).

Fixation : Paraformaldehyde fixed (4%, 15 min).

Permeabilization : Methanol permeabilized (90%, @ -20°C for >30 min).

Blocking : 1% BSA /PBS (20 min).

Primary : ab173006 0.5 μg/ml in 1% BSA/PBS, 1hr at room temperature.

Secondary : Alexa Fluor® 488 goat anti-mouse IgG1 used at a 1/1000 dilution in 1% BSA/PBS for 30 min at RT.

Washing : 2x 1% BSA/PBS.

Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB2] (AB173006)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB2] (AB173006)

Immunocytochemistry with HDFn cells stained with ab173006 (red) and DAPI (blue).

Fixation : Paraformaldehyde fixed (4%, 20 min).

Used heated antigen retrieval for optimal signal.

Permeabilization : Triton X-100 permeabilized (0.1%, 10min).

Blocking : 10% Goat Serum/PBS (1 hour).

Primary : ab173006 5 μg/ml (10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C).

Secondary : Alexa Fluor® 594 goat anti-mouse H+L used at a 1/1000 dilution in 1% Goat Serum/PBS for 1hr at RT.

Washing : 3X 1% Goat Serum/PBS, 10 mins/wash.

DAPI : 2 μM in 1% Goat Serum/PBS, 10 mins.

The target protein locates to the mitochondria (red).

Western blot - Anti-ATP5H antibody [10G5AB2] (AB173006)
  • WB

Supplier Data

Western blot - Anti-ATP5H antibody [10G5AB2] (AB173006)

All lanes:

Western blot - Anti-ATP5H antibody [10G5AB2] (ab173006) at 0.2 µg/mL

Lane 1:

Human Heart Homogenate at 20 µg

Lane 2:

Rat Heart Homogenate at 20 µg

Lane 3:

Mouse Heart Homogenate at 20 µg

Lane 4:

Bovine Heart Homogenate at 15 µg

Lane 5:

Hela cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

Predicted band size: 18 kDa

false

Western blot - Anti-ATP5H antibody [10G5AB2] (AB173006)
  • WB

Supplier Data

Western blot - Anti-ATP5H antibody [10G5AB2] (AB173006)

All lanes:

Western blot - Anti-ATP5H antibody [10G5AB2] (ab173006) at 0.5 µg/mL

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

H4IIE cell lysate at 20 µg

Lane 3:

MEF cell lysate at 20 µg

Lane 4:

NIH-3T3 cell lysate at 20 µg

Lane 5:

COS7 cell lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

Predicted band size: 18 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10G5AB2

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Cow, Human, African green monkey

Applications

WB, Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification notes
ab173006 is purified to near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5H also known as ATP synthase subunit d is a component of the ATP synthase complex an important enzyme found in the mitochondria. It weighs approximately 18.4 kDa. This protein is part of the F1F0 ATP synthase complex specifically the F0 domain. ATP5H is highly expressed in tissues with high energy demand such as skeletal muscle and cardiac muscle indicating its important role in the cellular energy production.
Biological function summary

ATP5H facilitates the production of ATP the energy currency of the cell. It is essential in the synthesis of ATP from ADP and inorganic phosphate driven by a proton gradient across the mitochondrial membrane. ATP5H is integral to the structure of the F1F0 ATP synthase complex ensuring efficient proton translocation and ATP synthesis. This subunit is critical for the overall function and stability of the ATP synthase complex.

Pathways

ATP5H plays a role in oxidative phosphorylation and energy metabolism pathways. This protein interacts closely with other ATP synthase subunits including ATP5B and ATP5O orchestrating the conversion of energy derived from nutrients to ATP. The oxidative phosphorylation pathway is central to energy production in eukaryotic cells and ATP5H forms an essential link in this process.

ATP5H is implicated in mitochondrial dysfunctions and related conditions such as mitochondrial myopathies. Alterations or deficiencies in ATP5H can disrupt ATP production potentially leading to neuromuscular disorders. Additionally connections to ATP5A1 another subunit of ATP synthase are noted. Dysregulation in these interactions can provide insight into the molecular mechanisms of these diseases providing potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
See full target information ATP5PD
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Life sciences 308:120930 PubMed36075471

2022

SARS-CoV-2 infection and replication kinetics in different human cell types: The role of autophagy, cellular metabolism and ACE2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia Silva Bartolomeo,Robertha Mariana Rodrigues Lemes,Rafael Leite Morais,Gabriela Cruz Pereria,Tamires Alves Nunes,Angelica Jardim Costa,Rui Monteiro de Barros Maciel,Carla Torres Braconi,Juliana Terzi Maricato,Luiz Mario Ramos Janini,Liria Hiromi Okuda,Kil Sun Lee,Carla Máximo Prado,Rodrigo Portes Ureshino,Roberta Sessa Stilhano

BMC genomics 20:569 PubMed31291894

2019

Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes.

Applications

Unspecified application

Species

Unspecified reactive species

Yanling Zhao,Xiaoli Lu,Zhipeng Cheng,Mengfang Tian,Yangzong Qiangba,Qiang Fu,Zili Ren
View all publications
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Product promise

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