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Mouse Monoclonal ATP5H antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat, African green monkey, Cow samples. Cited in 2 publications.


Images

Western blot - Anti-ATP5H antibody [10G5AB2] (AB173006), expandable thumbnail
  • Flow Cytometry - Anti-ATP5H antibody [10G5AB2] (AB173006), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB2] (AB173006), expandable thumbnail
  • Western blot - Anti-ATP5H antibody [10G5AB2] (AB173006), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
African green monkey
Expected
Tested
Expected
Cow
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat, African green monkey, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
0.5 µg/mL
Notes

-

Species
Mouse
Dilution info
0.5 µg/mL
Notes

-

Species
Rat
Dilution info
0.5 µg/mL
Notes

-

Species
African green monkey
Dilution info
0.5 µg/mL
Notes

-

Species
Cow
Dilution info
0.5 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Rat, African green monkey, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Alternative names

ATP5H, My032, ATP5PD, ATPase subunit d, ATP synthase peripheral stalk subunit d

Recommended products

Mouse Monoclonal ATP5H antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat, African green monkey, Cow samples. Cited in 2 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
10G5AB2
Concentration
Loading...
Purification notes

ab173006 is purified to near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATP5H also known as ATP synthase subunit d is a component of the ATP synthase complex an important enzyme found in the mitochondria. It weighs approximately 18.4 kDa. This protein is part of the F1F0 ATP synthase complex specifically the F0 domain. ATP5H is highly expressed in tissues with high energy demand such as skeletal muscle and cardiac muscle indicating its important role in the cellular energy production.

Biological function summary

ATP5H facilitates the production of ATP the energy currency of the cell. It is essential in the synthesis of ATP from ADP and inorganic phosphate driven by a proton gradient across the mitochondrial membrane. ATP5H is integral to the structure of the F1F0 ATP synthase complex ensuring efficient proton translocation and ATP synthesis. This subunit is critical for the overall function and stability of the ATP synthase complex.

Pathways

ATP5H plays a role in oxidative phosphorylation and energy metabolism pathways. This protein interacts closely with other ATP synthase subunits including ATP5B and ATP5O orchestrating the conversion of energy derived from nutrients to ATP. The oxidative phosphorylation pathway is central to energy production in eukaryotic cells and ATP5H forms an essential link in this process.

Associated diseases and disorders

ATP5H is implicated in mitochondrial dysfunctions and related conditions such as mitochondrial myopathies. Alterations or deficiencies in ATP5H can disrupt ATP production potentially leading to neuromuscular disorders. Additionally connections to ATP5A1 another subunit of ATP synthase are noted. Dysregulation in these interactions can provide insight into the molecular mechanisms of these diseases providing potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ATP5H antibody [10G5AB2] (ab173006), expandable thumbnail

    Western blot - Anti-ATP5H antibody [10G5AB2] (ab173006)

    All lanes: Western blot - Anti-ATP5H antibody [10G5AB2] (ab173006) at 0.2 µg/mL

    Lane 1: Human Heart Homogenate at 20 µg

    Lane 2: Rat Heart Homogenate at 20 µg

    Lane 3: Mouse Heart Homogenate at 20 µg

    Lane 4: Bovine Heart Homogenate at 15 µg

    Lane 5: Hela cell lysate at 20 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size: 18 kDa

  • Flow Cytometry - Anti-ATP5H antibody [10G5AB2] (ab173006), expandable thumbnail

    Flow Cytometry - Anti-ATP5H antibody [10G5AB2] (ab173006)

    Flow Cytometry with HeLa cells stained with ab173006 (blue) and isotype-specific negative control (red).

    Fixation: Paraformaldehyde fixed (4%, 15 min).

    Permeabilization: Methanol permeabilized (90%, @ -20°C for >30 min).

    Blocking: 1% BSA /PBS (20 min).

    Primary: ab173006 0.5 μg/ml in 1% BSA/PBS, 1hr at room temperature.

    Secondary: Alexa Fluor® 488 goat anti-mouse IgG1 used at a 1/1000 dilution in 1% BSA/PBS for 30 min at RT.

    Washing: 2x 1% BSA/PBS.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB2] (ab173006), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB2] (ab173006)

    Immunocytochemistry with HDFn cells stained with ab173006 (red) and DAPI (blue).

    Fixation: Paraformaldehyde fixed (4%, 20 min).

    Used heated antigen retrieval for optimal signal.

    Permeabilization: Triton X-100 permeabilized (0.1%, 10min).

    Blocking: 10% Goat Serum/PBS (1 hour).

    Primary: ab173006 5 μg/ml (10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C).

    Secondary: Alexa Fluor® 594 goat anti-mouse H+L used at a 1/1000 dilution in 1% Goat Serum/PBS for 1hr at RT.

    Washing: 3X 1% Goat Serum/PBS, 10 mins/wash.

    DAPI: 2 μM in 1% Goat Serum/PBS, 10 mins.

    The target protein locates to the mitochondria (red).

  • Western blot - Anti-ATP5H antibody [10G5AB2] (ab173006), expandable thumbnail

    Western blot - Anti-ATP5H antibody [10G5AB2] (ab173006)

    All lanes: Western blot - Anti-ATP5H antibody [10G5AB2] (ab173006) at 0.5 µg/mL

    Lane 1: HepG2 cell lysate at 20 µg

    Lane 2: H4IIE cell lysate at 20 µg

    Lane 3: MEF cell lysate at 20 µg

    Lane 4: NIH-3T3 cell lysate at 20 µg

    Lane 5: COS7 cell lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size: 18 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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