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AB110275

Anti-ATP5H antibody [7F9BG1]

5

(3 Reviews)

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(21 Publications)

Mouse Monoclonal ATP5H antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat, Cow, African green monkey samples. Cited in 21 publications.

View Alternative Names

ATP5H, My032, ATP5PD, ATPase subunit d, ATP synthase peripheral stalk subunit d

4 Images
Flow Cytometry - Anti-ATP5H antibody [7F9BG1] (AB110275)
  • Flow Cyt

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Flow Cytometry - Anti-ATP5H antibody [7F9BG1] (AB110275)

HeLa cells were stained with 1 µg/mL ab110275 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [7F9BG1] (AB110275)
  • ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [7F9BG1] (AB110275)

Mitochondrial localization of ATP5H. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110275 (5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).

Western blot - Anti-ATP5H antibody [7F9BG1] (AB110275)
  • WB

Supplier Data

Western blot - Anti-ATP5H antibody [7F9BG1] (AB110275)

All lanes:

Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275) at 1 µg/mL

Lane 1:

HDFn (human) cell lysates at 20 µg

Lane 2:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysates at 20 µg

Lane 3:

H4IIE (rat) cell lysates at 20 µg

Lane 4:

MEF (mouse) cell lysates at 20 µg

Lane 5:

bovine heart mitochondria lysates at 5 µg

Predicted band size: 18 kDa

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Western blot - Anti-ATP5H antibody [7F9BG1] (AB110275)
  • WB

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Western blot - Anti-ATP5H antibody [7F9BG1] (AB110275)

All lanes:

Western blot - Anti-ATP5H antibody [7F9BG1] (ab110275) at 1/1 dilution

Lane 1:

Human heart mitochondria at 10 µg

Lane 2:

Bovine heart mitochondria at 4 µg

Lane 3:

Rat heart mitochondria at 10 µg

Lane 4:

Mouse heart mitochondria at 10 µg

Lane 5:

HepG2 mitochondria at 20 µg

Predicted band size: 18 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7F9BG1

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Cow, Human, African green monkey

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Secondary Antibodies

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  • 15
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p>(heat-induced antigen-retrieval improvessignal)</p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>(heat-induced antigen-retrieval improvessignal)</p>" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

For mouse and rat samples, this antibody will only recognize ATP5H in purified mitochondrial samples. Mouse and rat cell lysates and tissue homogenates are not recommneded with this antibody.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110275 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5H also known as ATP synthase subunit d is a component of the ATP synthase complex an important enzyme found in the mitochondria. It weighs approximately 18.4 kDa. This protein is part of the F1F0 ATP synthase complex specifically the F0 domain. ATP5H is highly expressed in tissues with high energy demand such as skeletal muscle and cardiac muscle indicating its important role in the cellular energy production.
Biological function summary

ATP5H facilitates the production of ATP the energy currency of the cell. It is essential in the synthesis of ATP from ADP and inorganic phosphate driven by a proton gradient across the mitochondrial membrane. ATP5H is integral to the structure of the F1F0 ATP synthase complex ensuring efficient proton translocation and ATP synthesis. This subunit is critical for the overall function and stability of the ATP synthase complex.

Pathways

ATP5H plays a role in oxidative phosphorylation and energy metabolism pathways. This protein interacts closely with other ATP synthase subunits including ATP5B and ATP5O orchestrating the conversion of energy derived from nutrients to ATP. The oxidative phosphorylation pathway is central to energy production in eukaryotic cells and ATP5H forms an essential link in this process.

ATP5H is implicated in mitochondrial dysfunctions and related conditions such as mitochondrial myopathies. Alterations or deficiencies in ATP5H can disrupt ATP production potentially leading to neuromuscular disorders. Additionally connections to ATP5A1 another subunit of ATP synthase are noted. Dysregulation in these interactions can provide insight into the molecular mechanisms of these diseases providing potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit d, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (PubMed : 37244256). Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements (By similarity).
See full target information ATP5PD
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10719 PubMed39715749

2024

Preventing excessive autophagy protects from the pathology of mtDNA mutations in Drosophila melanogaster.

Applications

Unspecified application

Species

Unspecified reactive species

Najla El Fissi,Florian A Rosenberger,Kai Chang,Alissa Wilhalm,Tom Barton-Owen,Fynn M Hansen,Zoe Golder,David Alsina,Anna Wedell,Matthias Mann,Patrick F Chinnery,Christoph Freyer,Anna Wredenberg

Nature cell biology 25:1575-1589 PubMed37770567

2023

Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas J Kramer,Gyan Prakash,R Stefan Isaac,Karine Choquet,Iliana Soto,Boryana Petrova,Hope E Merens,Naama Kanarek,L Stirling Churchman

International journal of molecular sciences 24: PubMed37834071

2023

The Pro-Oncogenic Protein IF Promotes Proliferation of Anoxic Cancer Cells during Re-Oxygenation.

Applications

Unspecified application

Species

Unspecified reactive species

Riccardo Righetti,Silvia Grillini,Valentina Del Dotto,Anna Costanzini,Francesca Liuzzi,Claudia Zanna,Gianluca Sgarbi,Giancarlo Solaini,Alessandra Baracca

Disease models & mechanisms 16: PubMed37497665

2023

OPA1 deficiency impairs oxidative metabolism in cycling cells, underlining a translational approach for degenerative diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Aurélie M C Millet,Corentin Coustham,Camille Champigny,Marlène Botella,Christine Demeilliers,Anne Devin,Anne Galinier,Pascale Belenguer,Joel Bordeneuve-Guibé,Noélie Davezac

Biochimica et biophysica acta. Molecular basis of disease 1870:166879 PubMed37689158

2023

The pro-oncogenic protein IF does not contribute to the Warburg effect and is not regulated by PKA in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gianluca Sgarbi,Riccardo Righetti,Valentina Del Dotto,Silvia Grillini,Valentina Giorgio,Alessandra Baracca,Giancarlo Solaini

Nutrients 12: PubMed32993022

2020

Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Rocchina Vilella,Gianluca Sgarbi,Valeria Naponelli,Monia Savi,Leonardo Bocchi,Francesca Liuzzi,Riccardo Righetti,Federico Quaini,Caterina Frati,Saverio Bettuzzi,Giancarlo Solaini,Donatella Stilli,Federica Rizzi,Alessandra Baracca

American journal of physiology. Heart and circulat 318:H1516-H1524 PubMed32383992

2020

Contractile work directly modulates mitochondrial protein levels in human engineered heart tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Ronald Ng,Lorenzo R Sewanan,Allison L Brill,Paul Stankey,Xia Li,Yibing Qyang,Barbara E Ehrlich,Stuart G Campbell

Scientific reports 10:3571 PubMed32107448

2020

Endothelin-1 Mediated Decrease in Mitochondrial Gene Expression and Bioenergetics Contribute to Neurodegeneration of Retinal Ganglion Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Renuka M Chaphalkar,Dorota L Stankowska,Shaoqing He,Bindu Kodati,Nicole Phillips,Jude Prah,Shaohua Yang,Raghu R Krishnamoorthy

Redox biology 30:101429 PubMed31981894

2020

Codon optimization is an essential parameter for the efficient allotopic expression of mtDNA genes.

Applications

WB

Species

Human

Caitlin J Lewis,Bhavna Dixit,Elizabeth Batiuk,Carter J Hall,Matthew S O'Connor,Amutha Boominathan

The Journal of clinical investigation 128:4098-4114 PubMed30124467

2018

Mitochondrial reprogramming via ATP5H loss promotes multimodal cancer therapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Kwon-Ho Song,Jae-Hoon Kim,Young-Ho Lee,Hyun Cheol Bae,Hyo-Jung Lee,Seon Rang Woo,Se Jin Oh,Kyung-Mi Lee,Cassian Yee,Bo Wook Kim,Hanbyoul Cho,Eun Joo Chung,Joon-Yong Chung,Stephen M Hewitt,Tae-Wook Chung,Ki-Tae Ha,Young-Ki Bae,Chih-Ping Mao,Andrew Yang,T C Wu,Tae Woo Kim
View all publications
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