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AB122241

Anti-ATP5I antibody

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(8 Publications)

Rabbit Polyclonal ATP5I antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP5ME aa 1 to C-terminus.

View Alternative Names

ATP5I, ATP5K, ATP5ME, ATPase subunit e, ATP synthase membrane subunit e

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5I antibody (AB122241)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5I antibody (AB122241)

Immunohistochemical staining of human duodenum shows strong cytoplasmic granular positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5I antibody (AB122241)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5I antibody (AB122241)

Immunohistochemical staining of human testis shows strong cytoplasmic granular positivity in Leydig cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5I antibody (AB122241)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5I antibody (AB122241)

Immunohistochemical staining of human liver shows strong cytoplasmic granular positivity in hepatocytes.

Immunocytochemistry/ Immunofluorescence - Anti-ATP5I antibody (AB122241)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATP5I antibody (AB122241)

Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ATP5ME aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P56385

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5L antibody (AB126181)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab122241 is mono-specific.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5I also known as ATP synthase subunit e is a part of the mitochondrial ATP synthase complex. Its molecular weight is approximately 11 kDa. ATP5I plays a role in the formation of the F1Fo ATP synthase which is present in the inner mitochondrial membrane. This complex is essential for the synthesis of ATP the energy currency of the cell. ATP5I is expressed in tissues with high energy demands such as heart and skeletal muscle where it contributes to energy production.
Biological function summary

ATP5I functions as part of the ATP synthase complex also known as Complex V within the mitochondria. This protein is involved in the final step of oxidative phosphorylation where it aids in the conversion of ADP to ATP using the proton gradient across the inner mitochondrial membrane. The presence of ATP5I ensures the proper assembly and function of the ATP synthase complex facilitating efficient production of ATP necessary for cellular activities.

Pathways

The involvement of ATP5I in oxidative phosphorylation emphasizes its key role in cellular metabolism. This process is integral to the electron transport chain a critical pathway for energy harvesting in cells. ATP5I works in concert with other subunits like ATP5F1 and ATP5H which together form the functional ATP synthase complex. The efficiency of this pathway directly affects energy production within cells linking ATP5I to metabolic regulation.

Aberrations in ATP5I function can contribute to mitochondrial diseases such as Leigh syndrome and mitochondrial encephalomyopathy. These disorders are characterized by defects in energy metabolism due to impaired ATP synthesis. Associations of ATP5I with other components of the ATP synthase complex like ATP5A1 suggest that disruptions in these relationships can lead to a cascade of dysfunctional metabolic activities highlighting its importance in maintaining cellular energy homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit e, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (PubMed : 37244256).
See full target information ATP5ME
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 36:2130-2145.e7 PubMed39084216

2024

Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tongtong Wang,Anand Kumar Sharma,Chunyan Wu,Claudia Irene Maushart,Adhideb Ghosh,Wu Yang,Patrik Stefanicka,Zuzana Kovanicova,Jozef Ukropec,Jing Zhang,Myrtha Arnold,Manuel Klug,Katrien De Bock,Ulrich Schneider,Cristina Popescu,Bo Zheng,Lianggong Ding,Fen Long,Revati Sumukh Dewal,Caroline Moser,Wenfei Sun,Hua Dong,Martin Takes,Dominique Suelberg,Alexander Mameghani,Antonio Nocito,Christoph Johannes Zech,Alin Chirindel,Damian Wild,Irene A Burger,Michael R Schön,Arne Dietrich,Min Gao,Markus Heine,Yizhi Sun,Ariana Vargas-Castillo,Susanna Søberg,Camilla Scheele,Miroslav Balaz,Matthias Blüher,Matthias Johannes Betz,Bruce M Spiegelman,Christian Wolfrum

Molecular and cellular biology 44:226-244 PubMed38828998

2024

Reduced Protein Import via TIM23 SORT Drives Disease Pathology in TIMM50-Associated Mitochondrial Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan J Crameri,Catherine S Palmer,Tegan Stait,Thomas D Jackson,Matthew Lynch,Adriane Sinclair,Leah E Frajman,Alison G Compton,David Coman,David R Thorburn,Ann E Frazier,Diana Stojanovski

International journal of molecular sciences 22: PubMed34638634

2021

Proteomic Study of Low-Birth-Weight Nephropathy in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Toshiyuki Imasawa,Stéphane Claverol,Didier Lacombe,Nivea Dias Amoedo,Rodrigue Rossignol

Nature communications 12:4835 PubMed34376679

2021

Defining the molecular mechanisms of the mitochondrial permeability transition through genetic manipulation of F-ATP synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Carrer,Ludovica Tommasin,Justina Šileikytė,Francesco Ciscato,Riccardo Filadi,Andrea Urbani,Michael Forte,Andrea Rasola,Ildikò Szabò,Michela Carraro,Paolo Bernardi

Cell reports 35:108983 PubMed33852870

2021

A naturally occurring mutation in ATP synthase subunit c is associated with increased damage following hypoxia/reoxygenation in STEMI patients.

Applications

Unspecified application

Species

Unspecified reactive species

Giampaolo Morciano,Gaia Pedriali,Massimo Bonora,Rita Pavasini,Elisa Mikus,Simone Calvi,Matteo Bovolenta,Magdalena Lebiedzinska-Arciszewska,Mirko Pinotti,Alberto Albertini,Mariusz R Wieckowski,Carlotta Giorgi,Roberto Ferrari,Lorenzo Galluzzi,Gianluca Campo,Paolo Pinton

Cell reports 32:108095 PubMed32877677

2020

The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore.

Applications

Unspecified application

Species

Unspecified reactive species

Michela Carraro,Kristen Jones,Geppo Sartori,Marco Schiavone,Salvatore Antonucci,Roza Kucharczyk,Jean-Paul di Rago,Cinzia Franchin,Giorgio Arrigoni,Michael Forte,Paolo Bernardi

Nature communications 10:5823 PubMed31862883

2019

A mitochondrial megachannel resides in monomeric FF ATP synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Nelli Mnatsakanyan,Marc C Llaguno,Youshan Yang,Yangyang Yan,Joachim Weber,Fred J Sigworth,Elizabeth A Jonas

International journal of cancer 145:2157-2169 PubMed30924128

2019

Fibulin-7 is overexpressed in glioblastomas and modulates glioblastoma neovascularization through interaction with angiopoietin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Susana de Vega,Akihide Kondo,Mario Suzuki,Hajime Arai,Shabierjiang Jiapaer,Hemragul Sabit,Mitsutoshi Nakada,Tomoko Ikeuchi,Muneaki Ishijima,Eri Arikawa-Hirasawa,Yoshihiko Yamada,Yasunori Okada
View all publications
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