AB126181

Anti-ATP5L antibody

Name: Anti-ATP5L antibody (ab126181) | Abcam
Brand: Abcam Limited
SKU: AB126181
Price: 520 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal ATP5L antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP5MG aa 1-100.

View Alternative Names

ATP5L, ATP5MG, ATPase subunit g, ATP synthase membrane subunit g

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5L antibody (AB126181)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5L antibody (AB126181)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling ATP5L protein at mitochondria with ab126181 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Unknown

Western blot - Anti-ATP5L antibody (AB126181)

15% SDS PAGE

All lanes:

Western blot - Anti-ATP5L antibody (ab126181) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 11 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ATP5MG aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O75964

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5L also known as ATP synthase subunit f is a component of the mitochondrial ATP synthase complex. This protein is involved in the production of ATP the primary energy carrier within cells. The precise mass of human ATP5L is approximately 16 kDa. ATP5L is highly expressed in tissues with high energy demand such as the heart liver and skeletal muscle indicating its role in energy metabolism and mitochondrial functionality.

Biological function summary

ATP5L plays an essential part in ATP synthesis by facilitating the proton translocation across the mitochondrial membrane. It is a component of the F1F0 ATP synthase complex also known as Complex V of the electron transport chain. This complex synthesizes ATP from ADP and inorganic phosphate during oxidative phosphorylation an important process for cellular energy supply. Through its involvement in this complex ATP5L contributes to maintaining adequate levels of ATP within the cell.

Pathways

ATP5L acts within the oxidative phosphorylation and cellular respiration pathways. It collaborates with closely associated proteins like ATP5O and ATP5B in the energy production process. These pathways critically support cellular energy homeostasis and provide the energy required for various metabolic processes. ATP5L's function is thereby directly linked to the electron transport chain's efficient operation enabling the conversion of nutrient-derived energy into a usable cellular form.

Dysfunction in ATP5L has implications in metabolic-related disorders such as mitochondrial diseases and energy metabolism imbalances. ATP5L mutations or dysregulation can contribute to conditions like mitochondrial encephalomyopathy which affects the nervous system and muscles. Additionally the interaction of ATP5L with related proteins such as ATP5A1 can influence cardiac diseases where energy deficiencies lead to compromised heart function. Understanding these links offers insight into potential therapeutic targets for disorders related to energy metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Subunit g, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (PubMed : 37244256).

See full target information ATP5MG

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101647 PubMed39019006

2024

Dysregulation of FLVCR1a-dependent mitochondrial calcium handling in neural progenitors causes congenital hydrocephalus.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Bertino,Dibyanti Mukherjee,Massimo Bonora,Christoph Bagowski,Jeannette Nardelli,Livia Metani,Diletta Isabella Zanin Venturini,Diego Chianese,Nicolas Santander,Iris Chiara Salaroglio,Andreas Hentschel,Elisa Quarta,Tullio Genova,Arpana Arjun McKinney,Anna Lucia Allocco,Veronica Fiorito,Sara Petrillo,Giorgia Ammirata,Francesco De Giorgio,Evan Dennis,Garrett Allington,Felicitas Maier,Moneef Shoukier,Karl-Philipp Gloning,Luca Munaron,Federico Mussano,Ettore Salsano,Davide Pareyson,Maja di Rocco,Fiorella Altruda,Georgia Panagiotakos,Kristopher T Kahle,Pierre Gressens,Chiara Riganti,Paolo P Pinton,Andreas Roos,Thomas Arnold,Emanuela Tolosano,Deborah Chiabrando

Nature communications 12:4835 PubMed34376679

2021

Defining the molecular mechanisms of the mitochondrial permeability transition through genetic manipulation of F-ATP synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Carrer,Ludovica Tommasin,Justina Šileikytė,Francesco Ciscato,Riccardo Filadi,Andrea Urbani,Michael Forte,Andrea Rasola,Ildikò Szabò,Michela Carraro,Paolo Bernardi

Cell reports 35:108983 PubMed33852870

2021

A naturally occurring mutation in ATP synthase subunit c is associated with increased damage following hypoxia/reoxygenation in STEMI patients.

Applications

Unspecified application

Species

Unspecified reactive species

Giampaolo Morciano,Gaia Pedriali,Massimo Bonora,Rita Pavasini,Elisa Mikus,Simone Calvi,Matteo Bovolenta,Magdalena Lebiedzinska-Arciszewska,Mirko Pinotti,Alberto Albertini,Mariusz R Wieckowski,Carlotta Giorgi,Roberto Ferrari,Lorenzo Galluzzi,Gianluca Campo,Paolo Pinton

Cell reports 32:108095 PubMed32877677

2020

The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore.

Applications

Unspecified application

Species

Unspecified reactive species

Michela Carraro,Kristen Jones,Geppo Sartori,Marco Schiavone,Salvatore Antonucci,Roza Kucharczyk,Jean-Paul di Rago,Cinzia Franchin,Giorgio Arrigoni,Michael Forte,Paolo Bernardi

Nature communications 10:5823 PubMed31862883

2019

A mitochondrial megachannel resides in monomeric FF ATP synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Nelli Mnatsakanyan,Marc C Llaguno,Youshan Yang,Yangyang Yan,Joachim Weber,Fred J Sigworth,Elizabeth A Jonas

Oncogene 35:5000-9 PubMed26923328

2016

RNA-binding motif protein 47 inhibits Nrf2 activity to suppress tumor growth in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

T Sakurai,K Isogaya,S Sakai,M Morikawa,Y Morishita,S Ehata,K Miyazono,D Koinuma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ATP5L antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5L antibody (AB126181)

Western blot - Anti-ATP5L antibody (AB126181)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise