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Mouse Monoclonal ATP5O antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, African green monkey, Cow samples. Cited in 25 publications.


Images

Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276), expandable thumbnail
  • Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276), expandable thumbnail
  • Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (AB110276), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (AB110276), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (AB110276), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
African green monkey
Expected
Tested
Expected
Expected
Cow
Expected
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

African green monkey, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

African green monkey

Dilution info

0.5 µg/mL

Notes

-

Species

Human

Dilution info

0.5 µg/mL

Notes

-

Species

Cow

Dilution info

0.5 µg/mL

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

African green monkey, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.5-1 µg/mL

Notes

Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization.

Expected
Expected

Species

African green monkey, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization.

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Alternative names

Recommended products

Mouse Monoclonal ATP5O antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, African green monkey, Cow samples. Cited in 25 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

4C11C10D12

Purity

IgG fraction

Specificity

Antigen retrieval is required for ICC. Immediately before the permeabilization step, heat the coverslips for 10 minutes in 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes.

Light chain type

kappa

Concentration
Loading...
Purification notes

Near homogeneity as judged by SDS-PAGE. ab110276 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ATP5O also known as OLIGOMYCIN SENSITIVITY CONFERRING PROTEIN (OSCP) plays an important role mechanically in the function of mitochondria. It is a component of the F1F0 ATP synthase complex responsible for the generation of ATP from ADP using the proton gradient across the mitochondrial membrane. ATP5O assists in the stabilization of the enzyme structure. The target protein has a molecular mass of approximately 23 kDa. Expression of ATP5O occurs in various tissues especially in muscles and organs with high energy demands such as the heart and liver.

Biological function summary

ATP5O assists in the energy production process by associating with the mitochondrial ATP synthase complex. It interacts closely with other subunits to form the stator stalk which is vital in the maintenance of the enzyme’s structural integrity during ATP synthesis. The proper function of ATP5O ensures effective conversion of the chemiosmotic energy into chemical energy within the mitochondria making metabolism efficient and supporting cellular energy requirements. This makes its role in energy homeostasis invaluable.

Pathways

ATP5O integrates into oxidative phosphorylation a pathway critical for cellular respiration and energy production. It collaborates with other ATP synthase subunits as well as cytochrome c to complete the electron transport chain culminating the release of ATP for cellular function. ATP5O’s activity impacts cellular energy homeostasis and interacts with proteins like ATP5A and ATP5B which are also part of the ATP synthase complex. Its involvement ensures effective energy transfer and metabolic adaptation to cellular demands.

Associated diseases and disorders

ATP5O disruptions have links to mitochondrial disorders and neurodegenerative diseases. Mutations affecting ATP5O function can lead to compromised ATP production resulting in conditions like mitochondrial encephalomyopathy. Additionally dysfunctions in ATP5O may contribute to Alzheimer’s disease due to impaired energy metabolism. The link through disease pathways is often closely associated with proteins in the ATP synthase complex highlighting ATP5O's significance in maintaining mitochondrial and neuronal health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276), expandable thumbnail

    Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)

    False colour image of Western blot: Anti-ATP5O antibody [4C11C10D12] staining at 1 ug/ml, shown in green. In Western blot, ab110276 was shown to bind specifically to ATP5O. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution.

    All lanes: Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/mL

    Lane 1: Recombinant Human ATP5O protein (Recombinant Human ATP5O protein ab104549) cell lysate at 0.1 µg

    Lane 2: Recombinant Human ATP5O protein (Recombinant Human ATP5O protein ab104549) cell lysate at 0.5 µg

    Performed under reducing conditions.

    Predicted band size: 23 kDa

    Observed band size: 24 kDa

  • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276), expandable thumbnail

    Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)

    All lanes: Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 0.5 µg/mL

    Lane 1: Human heart mitochondria at 5 µg

    Lane 2: Bovine heart mitochondria at 1 µg

    Predicted band size: 23 kDa

  • Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (ab110276), expandable thumbnail

    Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (ab110276)

    HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (ab110276), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (ab110276)

    Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (ab110276), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (ab110276)

    Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

  • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276), expandable thumbnail

    Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)

    Secondary antibody: Goat-Anti-Mouse-IR800 1:4000

    All lanes: Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/mL

    Lane 1: HDFn (human) cell lysates at 20 µg

    Lane 2: COS7 (monkey) cell lysates at 20 µg

    Lane 3: H4IIE (rat) cell lysates at 20 µg

    Lane 4: MEF (mouse) cell lysates at 20 µg

    Lane 5: bovine heart mitochondria lysates at 5 µg

    Predicted band size: 23 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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