Mouse Monoclonal ATP5O antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, African green monkey, Cow samples. Cited in 25 publications.
IgG1
Mouse
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
Liquid
Monoclonal
Flow Cyt | WB | IHC-P | ICC/IF | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended |
African green monkey | Expected | Tested | Expected | Expected |
Cow | Expected | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info 0.5 µg/mL | Notes - |
Species Human | Dilution info 0.5 µg/mL | Notes - |
Species Cow | Dilution info 0.5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization. |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization. |
Species Mouse | Dilution info - | Notes - |
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Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
ATP5O, ATPO, ATPO, ATP5O, ATP5PO, ATP synthase peripheral stalk subunit OSCP, Oligomycin sensitivity conferral protein, OSCP
Mouse Monoclonal ATP5O antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, African green monkey, Cow samples. Cited in 25 publications.
IgG1
Mouse
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
Liquid
Monoclonal
4C11C10D12
IgG fraction
Antigen retrieval is required for ICC. Immediately before the permeabilization step, heat the coverslips for 10 minutes in 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes.
kappa
Near homogeneity as judged by SDS-PAGE. ab110276 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Blue Ice
+4°C
+4°C
Do Not Freeze
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Product was previously marketed under the MitoSciences sub-brand.
This supplementary information is collated from multiple sources and compiled automatically.
ATP5O also known as OLIGOMYCIN SENSITIVITY CONFERRING PROTEIN (OSCP) plays an important role mechanically in the function of mitochondria. It is a component of the F1F0 ATP synthase complex responsible for the generation of ATP from ADP using the proton gradient across the mitochondrial membrane. ATP5O assists in the stabilization of the enzyme structure. The target protein has a molecular mass of approximately 23 kDa. Expression of ATP5O occurs in various tissues especially in muscles and organs with high energy demands such as the heart and liver.
ATP5O assists in the energy production process by associating with the mitochondrial ATP synthase complex. It interacts closely with other subunits to form the stator stalk which is vital in the maintenance of the enzyme’s structural integrity during ATP synthesis. The proper function of ATP5O ensures effective conversion of the chemiosmotic energy into chemical energy within the mitochondria making metabolism efficient and supporting cellular energy requirements. This makes its role in energy homeostasis invaluable.
ATP5O integrates into oxidative phosphorylation a pathway critical for cellular respiration and energy production. It collaborates with other ATP synthase subunits as well as cytochrome c to complete the electron transport chain culminating the release of ATP for cellular function. ATP5O’s activity impacts cellular energy homeostasis and interacts with proteins like ATP5A and ATP5B which are also part of the ATP synthase complex. Its involvement ensures effective energy transfer and metabolic adaptation to cellular demands.
ATP5O disruptions have links to mitochondrial disorders and neurodegenerative diseases. Mutations affecting ATP5O function can lead to compromised ATP production resulting in conditions like mitochondrial encephalomyopathy. Additionally dysfunctions in ATP5O may contribute to Alzheimer’s disease due to impaired energy metabolism. The link through disease pathways is often closely associated with proteins in the ATP synthase complex highlighting ATP5O's significance in maintaining mitochondrial and neuronal health.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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False colour image of Western blot: Anti-ATP5O antibody [4C11C10D12] staining at 1 ug/ml, shown in green. In Western blot, ab110276 was shown to bind specifically to ATP5O. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution.
All lanes: Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/mL
Lane 1: Recombinant Human ATP5O protein (Recombinant Human ATP5O protein ab104549) cell lysate at 0.1 µg
Lane 2: Recombinant Human ATP5O protein (Recombinant Human ATP5O protein ab104549) cell lysate at 0.5 µg
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 24 kDa
All lanes: Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 0.5 µg/mL
Lane 1: Human heart mitochondria at 5 µg
Lane 2: Bovine heart mitochondria at 1 µg
Predicted band size: 23 kDa
HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.
Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
Secondary antibody: Goat-Anti-Mouse-IR800 1:4000
All lanes: Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/mL
Lane 1: HDFn (human) cell lysates at 20 µg
Lane 2: COS7 (monkey) cell lysates at 20 µg
Lane 3: H4IIE (rat) cell lysates at 20 µg
Lane 4: MEF (mouse) cell lysates at 20 µg
Lane 5: bovine heart mitochondria lysates at 5 µg
Predicted band size: 23 kDa
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