AB110276

Anti-ATP5O antibody [4C11C10D12]

Name: Anti-ATP5O antibody [4C11C10D12] (ab110276) | Abcam
Brand: Abcam Limited
SKU: AB110276
Price: 475 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(31 Publications)

Mouse Monoclonal ATP5O antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, African green monkey, Cow samples. Cited in 31 publications.

View Alternative Names

ATP5O, ATPO, ATP5PO, ATP synthase subunit O, Oligomycin sensitivity conferral protein, OSCP

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (AB110276)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (AB110276)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.

Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Flow Cyt

Unknown

Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (AB110276)

HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Unknown

Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276)

All lanes:

Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 0.5 µg/mL

Lane 1:

Human heart mitochondria at 5 µg

Lane 2:

Bovine heart mitochondria at 1 µg

Predicted band size: 23 kDa

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Supplier Data

Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Secondary antibody : Goat-Anti-Mouse-IR800 1 : 4000

All lanes:

Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/mL

Lane 1:

HDFn (human) cell lysates at 20 µg

Lane 2:

COS7 (monkey) cell lysates at 20 µg

Lane 3:

H4IIE (rat) cell lysates at 20 µg

Lane 4:

MEF (mouse) cell lysates at 20 µg

Lane 5:

bovine heart mitochondria lysates at 5 µg

Predicted band size: 23 kDa

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Lab

Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276)

False colour image of Western blot : Anti-ATP5O antibody [4C11C10D12] staining at 1 ug/ml, shown in green. In Western blot, ab110276 was shown to bind specifically to ATP5O. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) at 1/20000 dilution.

All lanes:

Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) at 1 µg/mL

Lane 1:

Recombinant Human ATP5O protein (<a href='/en-us/products/proteins-peptides/recombinant-human-atp5o-protein-ab104549'>ab104549</a>) cell lysate at 0.1 µg

Lane 2:

Recombinant Human ATP5O protein (<a href='/en-us/products/proteins-peptides/recombinant-human-atp5o-protein-ab104549'>ab104549</a>) cell lysate at 0.5 µg

Predicted band size: 23 kDa

Observed band size: 24 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ATP5O antibody [4C11C10D12]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4C11C10D12

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Cow, Human, African green monkey

Applications

IHC-P, WB, Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Antigen retrieval is required for ICC. Immediately before the permeabilization step, heat the coverslips for 10 minutes in 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-1 µg/mL", "ICCIF-species-notes": "Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "0.5-1 µg/mL", "ICCIF-species-notes": "Perform heat-induced antigen-retrieval with 100mM Tris, 5% urea, pH 9.5 at 95°C for 10 minutes immediately before permeabilization." }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Near homogeneity as judged by SDS-PAGE. ab110276 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP5O also known as OLIGOMYCIN SENSITIVITY CONFERRING PROTEIN (OSCP) plays an important role mechanically in the function of mitochondria. It is a component of the F1F0 ATP synthase complex responsible for the generation of ATP from ADP using the proton gradient across the mitochondrial membrane. ATP5O assists in the stabilization of the enzyme structure. The target protein has a molecular mass of approximately 23 kDa. Expression of ATP5O occurs in various tissues especially in muscles and organs with high energy demands such as the heart and liver.

Biological function summary

ATP5O assists in the energy production process by associating with the mitochondrial ATP synthase complex. It interacts closely with other subunits to form the stator stalk which is vital in the maintenance of the enzyme’s structural integrity during ATP synthesis. The proper function of ATP5O ensures effective conversion of the chemiosmotic energy into chemical energy within the mitochondria making metabolism efficient and supporting cellular energy requirements. This makes its role in energy homeostasis invaluable.

Pathways

ATP5O integrates into oxidative phosphorylation a pathway critical for cellular respiration and energy production. It collaborates with other ATP synthase subunits as well as cytochrome c to complete the electron transport chain culminating the release of ATP for cellular function. ATP5O’s activity impacts cellular energy homeostasis and interacts with proteins like ATP5A and ATP5B which are also part of the ATP synthase complex. Its involvement ensures effective energy transfer and metabolic adaptation to cellular demands.

ATP5O disruptions have links to mitochondrial disorders and neurodegenerative diseases. Mutations affecting ATP5O function can lead to compromised ATP production resulting in conditions like mitochondrial encephalomyopathy. Additionally dysfunctions in ATP5O may contribute to Alzheimer’s disease due to impaired energy metabolism. The link through disease pathways is often closely associated with proteins in the ATP synthase complex highlighting ATP5O's significance in maintaining mitochondrial and neuronal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit OSCP, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed : 37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed : 37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed : 37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (PubMed : 37244256). Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements (By similarity).

See full target information ATP5PO

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Diabetes, metabolic syndrome and obesity : targets and therapy 18:2317-2330 PubMed40672059

2025

Metformin Activation of Sirtuin 3 Signaling Regulates Mitochondrial Function Improves Diabetes-Associated Cognitive Impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Fei An,Hang Su,Chun-Qiang Zhang,Xue-Ting Wang,Guang-Qiong Zhang,Ling-Yun Fu,Yi-Ni Xu,Ling Tao,Xiang-Chun Shen

Communications biology 7:1486 PubMed39528709

2024

N-terminal cleavage of cyclophilin D boosts its ability to bind F-ATP synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Coluccino,Alessandro Negro,Antonio Filippi,Camilla Bean,Valentina Pia Muraca,Clarissa Gissi,Diana Canetti,Maria Chiara Mimmi,Elisa Zamprogno,Francesco Ciscato,Laura Acquasaliente,Vincenzo De Filippis,Marina Comelli,Michela Carraro,Andrea Rasola,Christoph Gerle,Paolo Bernardi,Alessandra Corazza,Giovanna Lippe

Biochimica et biophysica acta. Molecular basis of disease 1870:167131 PubMed38521420

2024

A stagewise response to mitochondrial dysfunction in mitochondrial DNA maintenance disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Vincent,Chun Chen,Tiago Bernardino Gomes,Valeria Di Leo,Tuomas Laalo,Kamil Pabis,Rodrick Capaldi,Michael F Marusich,David McDonald,Andrew Filby,Andrew Fuller,Diana Lehmann Urban,Stephan Zierz,Marcus Deschauer,Doug Turnbull,Amy K Reeve,Conor Lawless

Cell death and differentiation 29:2335-2346 PubMed35614131

2022

The mitochondrial chaperone TRAP1 regulates F-ATP synthase channel formation.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Cannino,Andrea Urbani,Marco Gaspari,Mariaconcetta Varano,Alessandro Negro,Antonio Filippi,Francesco Ciscato,Ionica Masgras,Christoph Gerle,Elena Tibaldi,Anna Maria Brunati,Giorgio Colombo,Giovanna Lippe,Paolo Bernardi,Andrea Rasola

International journal of biological sciences 18:2329-2344 PubMed35414767

2022

Claudin-10 overexpression suppresses human clear cell renal cell carcinoma growth and metastasis by regulating ATP5O and causing mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Wuping Yang,Kenan Zhang,Zedan Zhang,Jingcheng Zhou,Lei Li,Yawei Xu,Jianhui Qiu,Lin Cai,Yanqing Gong,Kan Gong

Cell death & disease 12:847 PubMed34518519

2021

Sirtuin 3 regulates mitochondrial protein acetylation and metabolism in tubular epithelial cells during renal fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Ping Wen,Jing Luo,Hao Ding,Hongdi Cao,Weichun He,Ke Zen,Yang Zhou,Junwei Yang,Lei Jiang

Nature communications 12:4835 PubMed34376679

2021

Defining the molecular mechanisms of the mitochondrial permeability transition through genetic manipulation of F-ATP synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Carrer,Ludovica Tommasin,Justina Šileikytė,Francesco Ciscato,Riccardo Filadi,Andrea Urbani,Michael Forte,Andrea Rasola,Ildikò Szabò,Michela Carraro,Paolo Bernardi

NPJ Parkinson's disease 7:39 PubMed33980828

2021

Imaging mass cytometry reveals generalised deficiency in OXPHOS complexes in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Chen,David McDonald,Alasdair Blain,Ashwin Sachdeva,Laura Bone,Anna L M Smith,Charlotte Warren,Sarah J Pickett,Gavin Hudson,Andrew Filby,Amy E Vincent,Doug M Turnbull,Amy K Reeve

Genomics, proteomics & bioinformatics 18:679-695 PubMed33607292

2021

Screening of Potential Biomarkers for Gastric Cancer with Diagnostic Value Using Label-free Global Proteome Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxi Song,Jun Wang,Jingxu Sun,Xiaowan Chen,Jinxin Shi,Zhonghua Wu,Dehao Yu,Fei Zhang,Zhenning Wang

Biochimica et biophysica acta. Molecular cell research 1868:118942 PubMed33359711

2020

TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel.

Applications

Unspecified application

Species

Unspecified reactive species

Hela Bahri,Jeremie Buratto,Manuel Rojo,Jim Paul Dompierre,Bénédicte Salin,Corinne Blancard,Sylvain Cuvellier,Marie Rose,Amel Ben Ammar Elgaaied,Emmanuel Tetaud,Jean-Paul di Rago,Anne Devin,Stéphane Duvezin-Caubet

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ATP5O antibody [4C11C10D12]

Immunocytochemistry/ Immunofluorescence - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Flow Cytometry - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Western blot - Anti-ATP5O antibody [4C11C10D12] (AB110276)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (31)

Product promise