Rabbit Polyclonal ATP6J/ATP6V1G1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1G1 aa 1 to C-terminus.
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Publications
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- Nature communications 8:16402017UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophia Maschalidi et. al.PubMed 29158474
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ATP6V1G1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75348
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Function
Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:32001091, PubMed:33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).
Alternative names
ATP6G, ATP6G1, ATP6J, ATP6V1G1, V-type proton ATPase subunit G 1, V-ATPase subunit G 1, V-ATPase 13 kDa subunit 1, Vacuolar proton pump subunit G 1, Vacuolar proton pump subunit M16
Recommended products
Rabbit Polyclonal ATP6J/ATP6V1G1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1G1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ATP6V1G1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75348
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ATP6J/ATP6V1G1 protein also known as V-type proton ATPase subunit G 1 is a component of the V1 domain of the vacuolar ATPase (V-ATPase) complex. The protein has a mass of roughly 13 kDa. It is expressed in various tissues with a higher abundance observed in the kidney and brain. The protein plays a role in the energy-dependent transport of protons across intracellular membranes an important component of cellular processes that require ATP hydrolysis.
- Biological function summary
ATP6V1G1 is part of the multi-subunit V-ATPase complex which functions as a proton pump to acidify intracellular compartments like lysosomes and endosomes. The complex enables the maintenance of microenvironments necessary for processes such as protein sorting zymogen activation and receptor-mediated endocytosis. By facilitating pH regulation within cells ATP6V1G1 supports essential cellular activities and homeostasis.
- Pathways
ATP6V1G1 integrates into the acidification pathway linked to intracellular organelles. It also interfaces with the mTOR signaling pathway which depends on lysosomal acidification for its activation. The cooperation between V-ATPase and mTOR relates to other binding proteins and complexes involved in cellular growth and autophagy such as LAMP2 and LC3.
- Associated diseases and disorders
Dysfunctions in ATP6V1G1-associated pathways contribute to lysosomal storage disorders and renal tubular acidosis. Impaired V-ATPase function affects lysosomal enzyme activation and cellular trafficking. Additionally altered interactions between ATP6V1G1 and cystinosin a protein involved in cystinosis link the V-ATPase to pathological states within the kidneys impacting cellular waste processing.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-ATP6J/ATP6V1G1 antibody (ab220044)
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized PC-3 cells labeling ATP6J/ATP6V1G1 with ab220044 at 4 μg/ml (green).
Western blot - Anti-ATP6J/ATP6V1G1 antibody (ab220044)
All lanes: Western blot - Anti-ATP6J/ATP6V1G1 antibody (ab220044) at 1/100 dilution
Lane 1: RT-4 cell lysate
Lane 2: U-251 MG cell lysate
Lane 3: Human plasma
Lane 4: Human liver lysate
Predicted band size: 14 kDa
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