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AB220044

Anti-ATP6J/ATP6V1G1 antibody

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(1 Publication)

Rabbit Polyclonal ATP6J/ATP6V1G1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1G1 aa 1 to C-terminus.

View Alternative Names

ATP6G, ATP6G1, ATP6J, ATP6V1G1, V-type proton ATPase subunit G 1, V-ATPase subunit G 1, V-ATPase 13 kDa subunit 1, Vacuolar proton pump subunit G 1, Vacuolar proton pump subunit M16

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ATP6J/ATP6V1G1 antibody (AB220044)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATP6J/ATP6V1G1 antibody (AB220044)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized PC-3 cells labeling ATP6J/ATP6V1G1 with ab220044 at 4 μg/ml (green).

Western blot - Anti-ATP6J/ATP6V1G1 antibody (AB220044)
  • WB

Supplier Data

Western blot - Anti-ATP6J/ATP6V1G1 antibody (AB220044)

All lanes:

Western blot - Anti-ATP6J/ATP6V1G1 antibody (ab220044) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ATP6V1G1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75348

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ATP6J/ATP6V1G1 protein also known as V-type proton ATPase subunit G 1 is a component of the V1 domain of the vacuolar ATPase (V-ATPase) complex. The protein has a mass of roughly 13 kDa. It is expressed in various tissues with a higher abundance observed in the kidney and brain. The protein plays a role in the energy-dependent transport of protons across intracellular membranes an important component of cellular processes that require ATP hydrolysis.
Biological function summary

ATP6V1G1 is part of the multi-subunit V-ATPase complex which functions as a proton pump to acidify intracellular compartments like lysosomes and endosomes. The complex enables the maintenance of microenvironments necessary for processes such as protein sorting zymogen activation and receptor-mediated endocytosis. By facilitating pH regulation within cells ATP6V1G1 supports essential cellular activities and homeostasis.

Pathways

ATP6V1G1 integrates into the acidification pathway linked to intracellular organelles. It also interfaces with the mTOR signaling pathway which depends on lysosomal acidification for its activation. The cooperation between V-ATPase and mTOR relates to other binding proteins and complexes involved in cellular growth and autophagy such as LAMP2 and LC3.

Dysfunctions in ATP6V1G1-associated pathways contribute to lysosomal storage disorders and renal tubular acidosis. Impaired V-ATPase function affects lysosomal enzyme activation and cellular trafficking. Additionally altered interactions between ATP6V1G1 and cystinosin a protein involved in cystinosis link the V-ATPase to pathological states within the kidneys impacting cellular waste processing.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 32001091, PubMed : 33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed : 32001091). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed : 28296633).
See full target information ATP6V1G1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 8:1640 PubMed29158474

2017

UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia Maschalidi,Paula Nunes-Hasler,Clarissa R Nascimento,Ignacio Sallent,Valérie Lannoy,Meriem Garfa-Traore,Nicolas Cagnard,Fernando E Sepulveda,Pablo Vargas,Ana-Maria Lennon-Duménil,Peter van Endert,Thierry Capiod,Nicolas Demaurex,Guillaume Darrasse-Jèze,Bénédicte Manoury
View all publications
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Product promise

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