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AB237611

Anti-ATP6V0A1 antibody

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(1 Publication)

Rabbit Polyclonal ATP6V0A1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V0A1 aa 50-300.

View Alternative Names

ATP6N1, ATP6N1A, VPP1, ATP6V0A1, V-type proton ATPase 116 kDa subunit a 1, V-ATPase 116 kDa subunit a 1, Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, Vacuolar adenosine triphosphatase subunit Ac116, Vacuolar proton pump subunit 1, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0A1 antibody (AB237611)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0A1 antibody (AB237611)

Paraffin-embedded human kidney tissue stained for ATP6V0A1 using ab237611 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ATP6V0A1 antibody (AB237611)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATP6V0A1 antibody (AB237611)

A549 (human lung carcinoma cell line) cells stained for ATP6V0A1 (green) using ab237611 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ATP6V0A1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

Q93050

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Primary Antibodies

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Anti-ATP6V0D1/P39 antibody [EPR18320-38]

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V0A1 also known as a subunit of the V0 domain of the vacuolar ATPase (V-ATPase) is a transmembrane protein involved in the acidification of intracellular compartments. It forms a component of the proton channel within the V-ATPase. This protein weighs approximately 95 kDa and can be found expressed in many tissues such as the kidney brain and liver. Its mechanical role involves contributing to proton translocation across cell membranes which is essential for various cellular activities.
Biological function summary

The protein participates in the ATP-dependent acidification of organelles like lysosomes and endosomes. As a part of a multi-subunit V-ATPase complex ATP6V0A1 helps maintain cellular pH balance and is essential for processes such as protein degradation and receptor-mediated endocytosis. Its activity influences the function of numerous cellular processes including ion transport and neurotransmitter release by regulating the pH in synaptic vesicles.

Pathways

ATP6V0A1 is involved in essential biological pathways such as the endocytic pathway and the lysosomal degradation pathway. Within these pathways V-ATPase in which ATP6V0A1 is a component interacts with several important proteins like ClC-5 which is involved in chloride ion transport. These interactions ensure the maintenance of the acidic environment necessary for enzyme activity and substrate processing within lysosomes and endosomes.

Mutations or dysfunction in ATP6V0A1 associate with pathologies such as osteoporosis and some forms of tumour growth. Abnormal activity of V-ATPase with connection to ATP6V0A1 may influence cancer cell behavior by altering the acidic microenvironment impacting cell invasion and metastasis. Additionally relationship with proteins like mTOR important in regulating cell growth and metabolism highlights its importance in disease development highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that transports protons across cellular membranes. V-ATPase is responsible for the acidification of various organelles, such as lysosomes, endosomes, the trans-Golgi network, and secretory granules, including synaptic vesicles (PubMed : 33065002, PubMed : 33833240, PubMed : 34909687). In certain cell types, can be exported to the plasma membrane, where it is involved in the acidification of the extracellular environment (By similarity). Required for assembly and activity of the vacuolar ATPase (By similarity). Through its action on compartment acidification, plays an essential role in neuronal development in terms of integrity and connectivity of neurons (PubMed : 33833240).
See full target information ATP6V0A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37298473

2023

Chondrocyte Thrombomodulin Protects against Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Kang,Ai-Lun Yang,Chao-Han Lai,Tsan-Ju Chen,Sung-Yen Lin,Yan-Hsiung Wang,Chau-Zen Wang,Edward M Conway,Hua-Lin Wu,Mei-Ling Ho,Je-Ken Chang,Chung-Hwan Chen,Tsung-Lin Cheng
View all publications
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Product promise

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