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AB97440

Anti-ATP6V0A4 antibody

4

(1 Review)

|

(4 Publications)

Rabbit Polyclonal ATP6V0A4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V0A4 aa 50-300.

View Alternative Names

ATP6N1B, ATP6N2, ATP6V0A4, V-type proton ATPase 116 kDa subunit a 4, V-ATPase 116 kDa isoform a 4, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4, Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform

1 Images
Western blot - Anti-ATP6V0A4 antibody (AB97440)
  • WB

Supplier Data

Western blot - Anti-ATP6V0A4 antibody (AB97440)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ATP6V0A4 antibody (ab97440) at 1/3000 dilution

Lane 1:

MCF-7 whole cell lysates at 30 µg

Lane 2:

MCF-7 whole membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 96 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ATP6V0A4 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

Q9HBG4

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Secondary Antibodies

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Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V0A4 also known by its alternate name V-ATPase 116 kDa subunit a isoform 4 plays a significant role in acidifying intracellular compartments. This protein is part of the vacuolar ATPase (V-ATPase) complex a proton pump that utilizes ATP hydrolysis to translocate protons across cellular membranes. ATP6V0A4 possesses a mass of approximately 116 kDa and sees expression mainly in the kidney particularly in the intercalated cells of the renal collecting duct. Its expression ensures efficient acid-base homeostasis by aiding the secretion of protons into the urinary space.
Biological function summary

ATP6V0A4 is essential for maintaining proper ion balance and pH regulation in the kidney. It is a component of the V-ATPase complex which comprises multiple subunits working together to pump protons. By participating in intracellular acidification this protein facilitates various processes like protein degradation receptor-mediated endocytosis and coupled transport. The function of the V-ATPase complex in which ATP6V0A4 resides ensures cell survival in acidic environments and supports cellular homeostasis.

Pathways

ATP6V0A4 plays a role in renal acidification and the regulation of systemic blood pH levels. It functions within the context of ion transport pathways in the kidney contributing to the bicarbonate reabsorption process. In these pathways ATP6V0A4 interacts with other components of the V-ATPase complex and associates with proteins like anion exchanger 1 (AE1) to help maintain electrolyte balance and acid-base equilibrium in the body.

Mutations in ATP6V0A4 connect to distal renal tubular acidosis (dRTA) a condition characterized by an inability to effectively acidify urine due to renal tubular dysfunction. This disorder can lead to metabolic acidosis failure to thrive and nephrocalcinosis. Additionally ATP6V0A4 may relate to autosomal recessive inheritance patterns indirectly involving various proteins in acid-base regulation pathways. Understanding these interactions provides potential avenues for therapeutic intervention in dRTA and related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). Involved in normal vectorial acid transport into the urine by the kidney (PubMed : 10973252, PubMed : 12414817).
See full target information ATP6V0A4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:366 PubMed37559594

2023

Identification of ATP6V0A4 as a potential biomarker in renal cell carcinoma using integrated bioinformatics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinming Xu,Jiahao Jiang,Cong Yin,Yan Wang,Bentao Shi

The Journal of biological chemistry 298:102395 PubMed35988642

2022

Isoform a4 of the vacuolar ATPase a subunit promotes 4T1-12B breast cancer cell-dependent tumor growth and metastasis in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Su,Michael P Collins,Christina M McGuire,Mohammed A Alshagawi,Mariam K Alamoudi,Zhen Li,Michael Forgac

The Journal of biological chemistry 294:11248-11258 PubMed31167791

2019

Isoform-specific gene disruptions reveal a role for the V-ATPase subunit a4 isoform in the invasiveness of 4T1-12B breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Christina M McGuire,Michael P Collins,GeHong Sun-Wada,Yoh Wada,Michael Forgac

Scientific reports 7:15944 PubMed29162837

2017

L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human Niemann-Pick type C disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroko Yanagisawa,Tomohiro Ishii,Kentaro Endo,Emiko Kawakami,Kazuaki Nagao,Toshiyuki Miyashita,Keiko Akiyama,Kazuhiko Watabe,Masaaki Komatsu,Daisuke Yamamoto,Yoshikatsu Eto
View all publications
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Product promise

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