Rabbit Polyclonal ATP6V0A4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V0A4 aa 50-300.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
WB | ICC/IF | |
---|---|---|
Human | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). Involved in normal vectorial acid transport into the urine by the kidney (PubMed:10973252, PubMed:12414817).
ATP6N1B, ATP6N2, ATP6V0A4, V-type proton ATPase 116 kDa subunit a 4, V-ATPase 116 kDa isoform a 4, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4, Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform
Rabbit Polyclonal ATP6V0A4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V0A4 aa 50-300.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
ATP6V0A4 also known by its alternate name V-ATPase 116 kDa subunit a isoform 4 plays a significant role in acidifying intracellular compartments. This protein is part of the vacuolar ATPase (V-ATPase) complex a proton pump that utilizes ATP hydrolysis to translocate protons across cellular membranes. ATP6V0A4 possesses a mass of approximately 116 kDa and sees expression mainly in the kidney particularly in the intercalated cells of the renal collecting duct. Its expression ensures efficient acid-base homeostasis by aiding the secretion of protons into the urinary space.
ATP6V0A4 is essential for maintaining proper ion balance and pH regulation in the kidney. It is a component of the V-ATPase complex which comprises multiple subunits working together to pump protons. By participating in intracellular acidification this protein facilitates various processes like protein degradation receptor-mediated endocytosis and coupled transport. The function of the V-ATPase complex in which ATP6V0A4 resides ensures cell survival in acidic environments and supports cellular homeostasis.
ATP6V0A4 plays a role in renal acidification and the regulation of systemic blood pH levels. It functions within the context of ion transport pathways in the kidney contributing to the bicarbonate reabsorption process. In these pathways ATP6V0A4 interacts with other components of the V-ATPase complex and associates with proteins like anion exchanger 1 (AE1) to help maintain electrolyte balance and acid-base equilibrium in the body.
Mutations in ATP6V0A4 connect to distal renal tubular acidosis (dRTA) a condition characterized by an inability to effectively acidify urine due to renal tubular dysfunction. This disorder can lead to metabolic acidosis failure to thrive and nephrocalcinosis. Additionally ATP6V0A4 may relate to autosomal recessive inheritance patterns indirectly involving various proteins in acid-base regulation pathways. Understanding these interactions provides potential avenues for therapeutic intervention in dRTA and related conditions.
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7.5% SDS PAGE
All lanes: Western blot - Anti-ATP6V0A4 antibody (ab97440) at 1/3000 dilution
All lanes: Hep G2 whole cell lysate at 30 µg
Predicted band size: 96 kDa
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