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AB230323

Anti-ATP6V0C antibody

3

(2 Reviews)

|

(1 Publication)

Rabbit Polyclonal ATP6V0C antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATP6V0C aa 100-150.

View Alternative Names

ATP6C, ATP6L, ATPL, ATP6V0C, V-type proton ATPase 16 kDa proteolipid subunit c, V-ATPase 16 kDa proteolipid subunit c, Vacuolar proton pump 16 kDa proteolipid subunit c

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0C antibody (AB230323)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0C antibody (AB230323)

Formalin-fixed, paraffin-embedded human kidney tissue stained for ATP6V0C using ab230323 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ATP6V0C antibody (AB230323)
  • WB

Supplier Data

Western blot - Anti-ATP6V0C antibody (AB230323)

All lanes:

Western blot - Anti-ATP6V0C antibody (ab230323) at 1/1000 dilution

All lanes:

NIH-3T3 (mouse embryo fibroblast cell line) whole cell lysate at 35 µg

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ATP6V0C aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P27449

Specificity

This ATP6V0C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-126 amino acids from the C-terminal region of human ATP6V0C.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V0C also known as V-type proton ATPase 16 kDa proteolipid subunit or V0C functions as an integral component of the proton pump that translocates protons across intracellular and plasma membranes. This subunit essential for the assembly and operation of the V-ATPase complex has a mass of approximately 16 kDa. It is widely expressed particularly in cells with high ATP demand such as kidney and brain cells where acid-base balance and nutrient transport are important.
Biological function summary

ATP6V0C is a critical player in cellular processes involving pH regulation and vesicular transport. The protein forms part of the V-ATPase complex which contributes to acidification of endosomes lysosomes and the Golgi apparatus. This acidification helps in protein sorting zymogen activation receptor-mediated endocytosis and generation of proton gradients across membranes. As part of this complex ATP6V0C works closely with other subunits to achieve proper function.

Pathways

ATP6V0C participates prominently in the endocytic and lysosomal pathways. It plays a role in maintaining the acidity of the lysosomal environment necessary for hydrolase activity. ATP6V0C operates in concert with other proteins such as ATP6V1A and ClC-7 to regulate proton transport and lysosomal function linking it closely to cellular energy metabolism and nutrient sensing pathways.

ATP6V0C's malfunction associates with conditions such as osteopetrosis and neurodegenerative disorders wherein defective lysosomal acidification leads to impaired cellular function. Osteoclasts responsible for bone resorption rely on ATP6V0C and its associated complex for mineral dissolution. Mutations or dysfunctions in ATP6V0C can lead to disease states by affecting proteins like ATP6V1B2 causing improper acidification and substrate processing within lysosomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 33065002, PubMed : 36074901). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments, and in some cell types, it is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity).
See full target information ATP6V0C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC genomics 20:363 PubMed31072381

2019

Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops.

Applications

Unspecified application

Species

Unspecified reactive species

Junlin Song,Chunde Wang
View all publications
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Product promise

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