Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free
- RabMAb
- Recombinant
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(1 Publication)
Rabbit Recombinant Monoclonal ATP6V0D1/P39 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
View Alternative Names
ATP6D, VPATPD, ATP6V0D1, V-type proton ATPase subunit d 1, V-ATPase subunit d 1, 32 kDa accessory protein, V-ATPase 40 kDa accessory protein, V-ATPase AC39 subunit, Vacuolar proton pump subunit d 1, p39
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ATP6V0D1/P39 with ab202897 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : - ab202897 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling ATP6V0D1/P39 with ab202897 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ATP6V0D1/P39 with ab202897 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) labeling ATP6V0D1/P39 with ab202897 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ATP6V0D1/P39 with ab202897 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : - ab202897 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP6V0D1/P39 with ab202897 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ATP6V0D1/P39 with ab202897 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse kidney tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320] (<a href='/en-us/products/primary-antibodies/atp6v0d1-p39-antibody-epr18320-ab202897'>ab202897</a>) at 1/2000 dilution
Lane 1:
Human fetal brain lysate at 10 µg
Lane 2:
Human fetal heart lysate at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
false
Exposure time: 10s
- IP
Supplier Data
Immunoprecipitation - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
ATP6V0D1/P39 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202897 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab202897 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab202897 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202897 in HeLa whole cell lysate.
Blocking and dilution buffer : 5% NFDM/TBST.
Exposure time : 10 seconds.
All lanes:
Immunoprecipitation - Anti-ATP6V0D1/P39 antibody [EPR18320] (<a href='/en-us/products/primary-antibodies/atp6v0d1-p39-antibody-epr18320-ab202897'>ab202897</a>)
Predicted band size: 40 kDa
Observed band size: 40 kDa
false
- WB
Supplier Data
Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320] (<a href='/en-us/products/primary-antibodies/atp6v0d1-p39-antibody-epr18320-ab202897'>ab202897</a>) at 1/2000 dilution
All lanes:
Human breast cancer lysate at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
false
Exposure time: 3min
- WB
Supplier Data
Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320] (<a href='/en-us/products/primary-antibodies/atp6v0d1-p39-antibody-epr18320-ab202897'>ab202897</a>) at 1/10000 dilution
Lane 1:
Human fetal kidney lysate at 20 µg
Lane 2:
HeLa (Human eithelial cells from cervix adenocarcinoma) cell lysate at 20 µg
Lane 3:
MCF7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg
Lane 4:
A431 (Human epidermoid carcinoma) cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 122 kDa,40 kDa,59 kDa,60 kDa,72 kDa
Observed band size: 40 kDa
false
Exposure time: 1min
- WB
Supplier Data
Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320] - BSA and Azide free (AB251385)
This data was developed using ab202897, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-ATP6V0D1/P39 antibody [EPR18320] (<a href='/en-us/products/primary-antibodies/atp6v0d1-p39-antibody-epr18320-ab202897'>ab202897</a>) at 1/2000 dilution
Lane 1:
Mouse brain lysate at 10 µg
Lane 2:
Mouse kidney lysate at 10 µg
Lane 3:
Rat kidney lysate at 10 µg
Lane 4:
C6 (Rat glial tumor cells) cell lysate at 10 µg
Lane 5:
RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate at 10 µg
Lane 6:
PC-12 (Rat adrenal gland pheochromocytoma) cell lysate at 10 µg
Lane 7:
NIH/3T3 (Mouse embryo fibroblast cells) cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
false
Exposure time: 5s
Reactivity data
Product details
ab251385 is the carrier-free version of ab202897.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATP6V0D1 impacts the acidification necessary for intracellular processes by being a component of the V-ATPase complex. This complex functions as a proton pump moving protons across membranes to regulate pH in cellular compartments like lysosomes and endosomes. Proper function of V-ATPase and thereby of ATP6V0D1 is important for cellular homeostasis and energy metabolism. Disruption of these processes could impair critical functions such as protein degradation and nutrient processing.
Pathways
ATP6V0D1 plays roles in key cellular pathways such as the glucose metabolism pathway and protein processing in the endoplasmic reticulum. In these pathways ATP6V0D1 works closely with other proteins like ATP6V1 subunits within the V-ATPase complex. Its involvement in these pathways highlights not only its importance in cellular energy balance but also in maintaining the structural integrity of cells under varying metabolic conditions.
Product protocols
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Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Journal of nanobiotechnology 22:800 PubMed39731111
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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