JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB194557

Anti-ATP6V0D2 antibody

4

(3 Reviews)

|

(3 Publications)

Mouse Polyclonal ATP6V0D2 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V0D2 aa 200 to C-terminus.

View Alternative Names

V-type proton ATPase subunit d 2, V-ATPase subunit d 2, Vacuolar proton pump subunit d 2, ATP6V0D2

2 Images
Western blot - Anti-ATP6V0D2 antibody (AB194557)
  • WB

Supplier Data

Western blot - Anti-ATP6V0D2 antibody (AB194557)

All lanes:

Western blot - Anti-ATP6V0D2 antibody (ab194557) at 1/500 dilution

All lanes:

Y79 cell lysate at 50 µg

Predicted band size: 40 kDa

false

Western blot - Anti-ATP6V0D2 antibody (AB194557)
  • WB

Supplier Data

Western blot - Anti-ATP6V0D2 antibody (AB194557)

Expected MW of immunogen is 33.7 kDa.

All lanes:

Western blot - Anti-ATP6V0D2 antibody (ab194557) at 1/1000 dilution

All lanes:

recombinant immunogen at 0.2 µg

Predicted band size: 40 kDa,81 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ATP6V0D2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N8Y2

style
left-column

Alternative Products

Primary Antibodies

AB236375

Anti-ATP6V0D2 antibody [OTI2D6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D2 antibody [OTI2D6] (AB236375)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB202899

Anti-ATP6V0D1/P39 antibody [EPR18320-38]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [EPR18320-38] (AB202899)

  • 0
  • 0
Primary Antibodies

AB214672

Anti-c-Fos antibody [EPR20769]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR20769] (AB214672)

  • 5
  • 3
Primary Antibodies

AB32042

Anti-Cleaved Caspase-3 antibody [E83-77]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] (AB32042)

  • 4
  • 28
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V0D2 also known as V-type proton ATPase subunit d 2 is a subunit of the V-ATPase complex. It has a mass of approximately 38 kDa. This protein is expressed in various tissues with significant expression noted in bone and white adipose tissue. Mechanically ATP6V0D2 contributes to the acidification of intracellular compartments. It functions as part of the proton pump machinery facilitating the transfer of protons across membranes which is essential for processes like endocytosis and intracellular trafficking.
Biological function summary

ATP6V0D2 plays a significant role in bone resorption and adipogenesis. It is a component of the larger V-ATPase complex important for creating acidic environments required for enzyme activity particularly in osteoclasts. This acidity enables the breakdown of bone matrix which is a fundamental part of bone remodeling. Additionally ATP6V0D2 influences fat cell differentiation by modulating the function of cytosolic components within the complex.

Pathways

ATP6V0D2 functions as an integral part of the bone remodeling and lysosomal degradation pathways. In the bone remodeling pathway the protein works closely with other proton pump components like ATP6V0A3 to regulate osteoclast activity. In lysosomal degradation it aids in maintaining the acidity essential for lysosomal enzyme activity. Both pathways are connected through ATP6V0D2's regulation of intracellular pH and ion homeostasis.

ATP6V0D2 has associations with osteoporosis and obesity. In osteoporosis deficient or dysfunctional ATP6V0D2 activity can impair osteoclast function leading to altered bone resorption and skeletal fragility. In the context of obesity this protein influences adipocyte differentiation and function. ATP6V0D2's role in these disorders connects it to other proteins like RANKL in bone metabolism and PPARγ in adipogenesis linking it beyond just its structural function within proton pumps.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). May play a role in coupling of proton transport and ATP hydrolysis (By similarity). Regulator of osteoclast fusion and bone formation (By similarity).
See full target information ATP6V0D2
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and molecular physiology 327:L749-L755 PubMed39254087

2024

The pH-sensitive translocation of V-ATPase in the small airway of cystic fibrosis pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Raul Villacreses,Ian M Thornell,Xiaopeng Li,Steven E Mather,Jimena Urbano,David A Stoltz,Joseph Zabner

The Journal of clinical investigation 129:631-646 PubMed30431439

2019

Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2α-mediated tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Na Liu,Jing Luo,Dong Kuang,Sanpeng Xu,Yaqi Duan,Yu Xia,Zhengping Wei,Xiuxiu Xie,Bingjiao Yin,Fang Chen,Shunqun Luo,Huicheng Liu,Jing Wang,Kan Jiang,Feili Gong,Zhao-Hui Tang,Xiang Cheng,Huabin Li,Zhuoya Li,Arian Laurence,Guoping Wang,Xiang-Ping Yang

International journal of molecular medicine 42:2071-2079 PubMed30066839

2018

Vγ9Vδ2 T cells inhibit immature dendritic cell transdifferentiation into osteoclasts through downregulation of RANK, c‑Fos and ATP6V0D2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Zhu,Zhiyong Zeng,Dongbiao Qiu,Junmin Chen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com