JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB113745

Anti-ATP6V1A antibody [8B1F3]

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Monoclonal ATP6V1A antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 2 publications.

View Alternative Names

CLS8, TFP1, YDL185W, D1286, VMA1, V-type proton ATPase catalytic subunit A, V-ATPase subunit A, Vacuolar proton pump subunit A

1 Images
Western blot - Anti-ATP6V1A antibody [8B1F3] (AB113745)
  • WB

Unknown

Western blot - Anti-ATP6V1A antibody [8B1F3] (AB113745)

All lanes:

Western blot - Anti-ATP6V1A antibody [8B1F3] (ab113745) at 1 µg/mL

All lanes:

Yeast Whole Cell at 15 µg

Predicted band size: 68 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8B1F3

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V1A also known as V-ATPase subunit A is a component of the V1 domain of the vacuolar ATPase (V-ATPase) which is a large enzyme responsible for acidifying intracellular compartments. This protein has a mass of approximately 70 kDa and operates in cellular processes by hydrolyzing ATP to drive protons across membranes. ATP6V1A displays significant expression in several cell types including neurons epithelial cells and osteoclasts. The protein enables V-ATPase functionality important for maintaining the pH balance inside cells and organelles facilitating various physiological processes.
Biological function summary

ATP6V1A is an integral part of the V1 domain of the V-ATPase complex a multi-subunit assembly critical for the acidification of intracellular organelles such as lysosomes endosomes and the Golgi apparatus. This activity supports processes like protein degradation receptor-mediated endocytosis and membrane trafficking. The V-ATPase containing ATP6V1A uses energy from ATP hydrolysis to pump protons which is essential for cellular homeostasis and ion transport.

Pathways

ATP6V1A plays an important role in the endocytosis and autophagy pathways. These pathways depend on the acidification of intracellular compartments enabling processes such as the breakdown and recycling of cellular components. ATP6V1A is related to proteins like V-ATPase subunit c (ATP6V1C) and other subunits that form the V-ATPase enzyme. These protein interactions allow the modulation and execution of cellular transport and hormone regulation pathways.

Mutations or dysregulation of ATP6V1A have been linked to renal tubular acidosis and osteoporosis where improper acidification impacts bone resorption and kidney function. The protein connections include its interaction with other V-ATPase subunits like ATP6V0A3 which can also result in osteopetrosis when defective. Such conditions demonstrate how ATP6V1A's role in acidification processes is important for normal physiological function and how its dysfunction leads to disease.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 18055462, PubMed : 2139027). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments (PubMed : 18055462, PubMed : 2139027).. PI-SceI is an endonuclease that can cleave at a site present in a VMA1 allele that lacks the derived endonuclease segment of the open reading frame; cleavage at this site only occurs during meiosis and initiates 'homing', a genetic event that converts a VMA1 allele lacking VDE into one that contains it.
See full target information VMA1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 130:2317-2328 PubMed28576969

2017

Redox regulation of the yeast voltage-gated Ca channel homolog Cch1p by glutathionylation of specific cysteine residues.

Applications

Unspecified application

Species

Unspecified reactive species

Avinash Chandel,Anand K Bachhawat

Molecular biology of the cell 27:3913-3925 PubMed27708136

2016

Glutathione depletion activates the yeast vacuolar transient receptor potential channel, Yvc1p, by reversible glutathionylation of specific cysteines.

Applications

IF

Species

Unspecified reactive species

Avinash Chandel,Krishna K Das,Anand K Bachhawat
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB137574

Anti-ATP6V1A antibody

Immunoprecipitation - Anti-ATP6V1A antibody (AB137574)

  • 4
  • 1
Primary Antibodies

AB209823

Anti-ATP6V1A antibody

IHC - Wholemount - Anti-ATP6V1A antibody (AB209823)

  • 0
  • 0
Primary Antibodies

AB199325

Anti-ATP6V1A antibody [EPR19271]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1A antibody [EPR19271] (AB199325)

  • 0
  • 0
Primary Antibodies

AB199326

Anti-ATP6V1A antibody [EPR19270]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1A antibody [EPR19270] (AB199326)

  • 4
  • 2

Complementary Products

Primary Antibodies

AB202899

Anti-ATP6V0D1/P39 antibody [EPR18320-38]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V0D1/P39 antibody [EPR18320-38] (AB202899)

  • 0
  • 0
Primary Antibodies

AB73404

Anti-ATP6V1B2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1B2 antibody (AB73404)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB14730

Anti-ATPB antibody [3D5] - Mitochondrial Marker

Lab Essentials

Western blot - Anti-ATPB antibody [3D5] - Mitochondrial Marker (AB14730)

  • 5
  • 42

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com