Rabbit Polyclonal ATP6V1A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1A aa 50-350.
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- Nature communications 14:12592023Mucociliary Wnt signaling promotes cilia biogenesis and beating.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarina Seidl,Fabio Da Silva,Kaiqing Zhang,Kai Wohlgemuth,Heymut Omran,Christof NiehrsPubMed 36878953
- Nature communications 12:21072021ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H-ATPases is essential for brain development in humans and mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazushi Aoto,Mitsuhiro Kato,Tenpei Akita,Mitsuko Nakashima,Hiroki Mutoh,Noriyuki Akasaka,Jun Tohyama,Yoshiko Nomura,Kyoko Hoshino,Yasuhiko Ago,Ryuta Tanaka,Orna Epstein,Revital Ben-Haim,Eli Heyman,Takehiro Miyazaki,Hazrat Belal,Shuji Takabayashi,Chihiro Ohba,Atsushi Takata,Takeshi Mizuguchi,Satoko Miyatake,Noriko Miyake,Atsuo Fukuda,Naomichi Matsumoto,Hirotomo SaitsuPubMed 33833240
- Allergy, asthma & immunology research 13:479-4972021ATP6V0d2 Suppresses Alveoli Macrophage Alternative Polarization and Allergic Asthma via Degradation of PU.1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Liu,Yuchen Feng,Huicheng Liu,Wenliang Wu,Yuxia Liang,Pingfei Li,Zhengping Wei,Min Wu,Zhao Hui Tang,Junyan Han,Xiang Cheng,Zheng Liu,Arian Laurence,Huabin Li,Guohua Zhen,Xiang Ping YangPubMed 33733641
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e212232021The lysosomal membrane protein Sidt2 is a vital regulator of mitochondrial quality control in skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLizhuo Wang,Cui Yu,Wenjun Pei,Mengya Geng,Yao Zhang,Zihui Li,Feiteng Liang,Fengbiao Tan,Hui Du,Jialin GaoPubMed 33715196
- Frontiers in microbiology 11:5738442020and Escape From the Clearance of Macrophages via Controlling TFEB.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanshan Rao et. al.PubMed 33324360
- Autophagy 17:1998-20102020ATM loss disrupts the autophagy-lysosomal pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAifang Cheng,Kai-Hei Tse,Hei-Man Chow,Yunqiao Gan,Xuan Song,Fulin Ma,Yi Xuan Yvonne Qian,Weiyi She,Karl HerrupPubMed 32757690
- The Journal of nutritional biochemistry 73:1082142019Formononetin alleviates hepatic steatosis by facilitating TFEB-mediated lysosome biogenesis and lipophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Wang et. al.PubMed 31520816
- Nature chemical biology 15:776-7852019Covalent targeting of the vacuolar H-ATPase activates autophagy via mTORC1 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClive Yik-Sham Chung et. al.PubMed 31285595
- The Journal of experimental medicine 216:2091-21122019Thioesterase PPT1 balances viral resistance and efficient T cell crosspriming in dendritic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengju Ou et. al.PubMed 31262842
- EBioMedicine 45:408-4212019A subunit of V-ATPases, ATP6V1B2, underlies the pathology of intellectual disability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeihao Zhao et. al.PubMed 31257146
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ATP6V1A aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link P38606
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 - 1/20000 | Notes - |
Species Human | Dilution info 1/5000 - 1/20000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:8463241). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633). May play a role in neurite development and synaptic connectivity (PubMed:29668857). (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction.
Alternative names
ATP6A1, ATP6V1A1, VPP2, ATP6V1A, V-type proton ATPase catalytic subunit A, V-ATPase subunit A, V-ATPase 69 kDa subunit, Vacuolar ATPase isoform VA68, Vacuolar proton pump subunit alpha
Recommended products
Rabbit Polyclonal ATP6V1A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1A aa 50-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ATP6V1A aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link P38606
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP6V1A also known as V-ATPase subunit A is a component of the V1 domain of the vacuolar ATPase (V-ATPase) which is a large enzyme responsible for acidifying intracellular compartments. This protein has a mass of approximately 70 kDa and operates in cellular processes by hydrolyzing ATP to drive protons across membranes. ATP6V1A displays significant expression in several cell types including neurons epithelial cells and osteoclasts. The protein enables V-ATPase functionality important for maintaining the pH balance inside cells and organelles facilitating various physiological processes.
- Biological function summary
ATP6V1A is an integral part of the V1 domain of the V-ATPase complex a multi-subunit assembly critical for the acidification of intracellular organelles such as lysosomes endosomes and the Golgi apparatus. This activity supports processes like protein degradation receptor-mediated endocytosis and membrane trafficking. The V-ATPase containing ATP6V1A uses energy from ATP hydrolysis to pump protons which is essential for cellular homeostasis and ion transport.
- Pathways
ATP6V1A plays an important role in the endocytosis and autophagy pathways. These pathways depend on the acidification of intracellular compartments enabling processes such as the breakdown and recycling of cellular components. ATP6V1A is related to proteins like V-ATPase subunit c (ATP6V1C) and other subunits that form the V-ATPase enzyme. These protein interactions allow the modulation and execution of cellular transport and hormone regulation pathways.
- Associated diseases and disorders
Mutations or dysregulation of ATP6V1A have been linked to renal tubular acidosis and osteoporosis where improper acidification impacts bone resorption and kidney function. The protein connections include its interaction with other V-ATPase subunits like ATP6V0A3 which can also result in osteopetrosis when defective. Such conditions demonstrate how ATP6V1A's role in acidification processes is important for normal physiological function and how its dysfunction leads to disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-ATP6V1A antibody (ab137574)
7.5% SDS PAGE
All lanes: Western blot - Anti-ATP6V1A antibody (ab137574) at 1/10000 dilution
Lane 1: Hela whole cell lysate at 30 µg
Lane 2: Hep G2 whole cell lysate at 30 µg
Predicted band size: 68 kDa
Western blot - Anti-ATP6V1A antibody (ab137574)
7.5% SDS PAGE
All lanes: Western blot - Anti-ATP6V1A antibody (ab137574) at 1/5000 dilution
All lanes: Mouse brain whole cell lysate at 20 µg
Predicted band size: 68 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1A antibody (ab137574)
Immunofluorescence analysis of methanol-fixed A431 cells labelled with ab137574 at 1/500 dilution. Costained with Hoechst 33342.
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