AB137574

Anti-ATP6V1A antibody

Name: Anti-ATP6V1A antibody (ab137574) | Abcam
Brand: Abcam Limited
SKU: AB137574
Rating: 4 (1 reviews)

(1 Review)

(20 Publications)

Rabbit Polyclonal ATP6V1A antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1A aa 50-350.

View Alternative Names

ATP6A1, ATP6V1A1, VPP2, ATP6V1A, V-type proton ATPase catalytic subunit A, V-ATPase subunit A, V-ATPase 69 kDa subunit, Vacuolar ATPase isoform VA68, Vacuolar proton pump subunit alpha

4 Images

Supplier Data

Immunoprecipitation - Anti-ATP6V1A antibody (AB137574)

Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 µg of ATP6V1A antibody. Western blot analysis was performed using ATP6V1A antibody.

All lanes:

Immunoprecipitation - Anti-ATP6V1A antibody (ab137574) at 5 µg

Lane 1:

HeLa whole cell extracts (Input)

Lane 2:

HeLa whole cell extracts with control IgG

Lane 3:

HeLa whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-ATP6V1A antibody (AB137574)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-ATP6V1A antibody (ab137574) at 1/5000 dilution

Lane 1:

ATP6V1A shRNA transfected 293T whole cell extracts at 30 µg

Lane 2:

Non-transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-ATP6V1A antibody (AB137574)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-ATP6V1A antibody (ab137574) at 1/10000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 68 kDa

false

Unknown

Western blot - Anti-ATP6V1A antibody (AB137574)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ATP6V1A antibody (ab137574) at 1/5000 dilution

All lanes:

Mouse brain whole cell lysate at 20 µg

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human ATP6V1A aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

P38606

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V1A also known as V-ATPase subunit A is a component of the V1 domain of the vacuolar ATPase (V-ATPase) which is a large enzyme responsible for acidifying intracellular compartments. This protein has a mass of approximately 70 kDa and operates in cellular processes by hydrolyzing ATP to drive protons across membranes. ATP6V1A displays significant expression in several cell types including neurons epithelial cells and osteoclasts. The protein enables V-ATPase functionality important for maintaining the pH balance inside cells and organelles facilitating various physiological processes.

Biological function summary

ATP6V1A is an integral part of the V1 domain of the V-ATPase complex a multi-subunit assembly critical for the acidification of intracellular organelles such as lysosomes endosomes and the Golgi apparatus. This activity supports processes like protein degradation receptor-mediated endocytosis and membrane trafficking. The V-ATPase containing ATP6V1A uses energy from ATP hydrolysis to pump protons which is essential for cellular homeostasis and ion transport.

Pathways

ATP6V1A plays an important role in the endocytosis and autophagy pathways. These pathways depend on the acidification of intracellular compartments enabling processes such as the breakdown and recycling of cellular components. ATP6V1A is related to proteins like V-ATPase subunit c (ATP6V1C) and other subunits that form the V-ATPase enzyme. These protein interactions allow the modulation and execution of cellular transport and hormone regulation pathways.

Mutations or dysregulation of ATP6V1A have been linked to renal tubular acidosis and osteoporosis where improper acidification impacts bone resorption and kidney function. The protein connections include its interaction with other V-ATPase subunits like ATP6V0A3 which can also result in osteopetrosis when defective. Such conditions demonstrate how ATP6V1A's role in acidification processes is important for normal physiological function and how its dysfunction leads to disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 8463241). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed : 32001091). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed : 28296633). May play a role in neurite development and synaptic connectivity (PubMed : 29668857).. (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction.

See full target information ATP6V1A

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:111515 PubMed39758816

2025

TBC1D24 interacts with the v-ATPase and regulates intraorganellar pH in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Pepe,Davide Aprile,Enrico Castroflorio,Antonella Marte,Simone Giubbolini,Samir Hopestone,Anna Parsons,Tânia Soares,Fabio Benfenati,Peter L Oliver,Anna Fassio

Cellular and molecular life sciences : CMLS 81:416 PubMed39367928

2024

Apache is a neuronal player in autophagy required for retrograde axonal transport of autophagosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Parisi,Alessandro Esposito,Enrico Castroflorio,Mattia Bramini,Sara Pepe,Antonella Marte,Fabrizia C Guarnieri,Flavia Valtorta,Pietro Baldelli,Fabio Benfenati,Anna Fassio,Silvia Giovedì

Acta physiologica (Oxford, England) 240:e14186 PubMed38837572

2024

ATP6V1A is required for synaptic rearrangements and plasticity in murine hippocampal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Esposito,Sara Pepe,Maria Sabina Cerullo,Katia Cortese,Hanako Tsushima Semini,Silvia Giovedì,Renzo Guerrini,Fabio Benfenati,Antonio Falace,Anna Fassio

Nature communications 14:1259 PubMed36878953

2023

Mucociliary Wnt signaling promotes cilia biogenesis and beating.

Applications

Unspecified application

Species

Unspecified reactive species

Carina Seidl,Fabio Da Silva,Kaiqing Zhang,Kai Wohlgemuth,Heymut Omran,Christof Niehrs

Cell reports 41:111508 PubMed36288714

2022

Genome-wide CRISPR screen reveals v-ATPase as a drug target to lower levels of ALS protein ataxin-2.

Applications

Unspecified application

Species

Unspecified reactive species

Garam Kim,Lisa Nakayama,Jacob A Blum,Tetsuya Akiyama,Steven Boeynaems,Meenakshi Chakraborty,Julien Couthouis,Eduardo Tassoni-Tsuchida,Caitlin M Rodriguez,Michael C Bassik,Aaron D Gitler

Brain : a journal of neurology 145:2687-2703 PubMed35675510

2022

Phenotypic and genetic spectrum of ATP6V1A encephalopathy: a disorder of lysosomal homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Renzo Guerrini,Davide Mei,Katalin Kerti-Szigeti,Sara Pepe,Mary Kay Koenig,Gretchen Von Allmen,Megan T Cho,Kimberly McDonald,Janice Baker,Vikas Bhambhani,Zöe Powis,Lance Rodan,Rima Nabbout,Giulia Barcia,Jill A Rosenfeld,Carlos A Bacino,Cyril Mignot,Lillian H Power,Catharine J Harris,Dragan Marjanovic,Rikke S Møller,Trine B Hammer,Riikka Keski Filppula,Päivi Vieira,Clara Hildebrandt,Stephanie Sacharow,Luca Maragliano,Fabio Benfenati,Katherine Lachlan,Andreas Benneche,Florence Petit,Jean Madeleine de Sainte Agathe,Barbara Hallinan,Yue Si,Ingrid M Wentzensen,Fanggeng Zou,Vinodh Narayanan,Naomichi Matsumoto,Alessandra Boncristiano,Giancarlo la Marca,Mitsuhiro Kato,Kristin Anderson,Carmen Barba,Luisa Sturiale,Domenico Garozzo,Roberto Bei,Laura Masuelli,Valerio Conti,Gaia Novarino,Anna Fassio

Nature communications 12:2107 PubMed33833240

2021

ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H-ATPases is essential for brain development in humans and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kazushi Aoto,Mitsuhiro Kato,Tenpei Akita,Mitsuko Nakashima,Hiroki Mutoh,Noriyuki Akasaka,Jun Tohyama,Yoshiko Nomura,Kyoko Hoshino,Yasuhiko Ago,Ryuta Tanaka,Orna Epstein,Revital Ben-Haim,Eli Heyman,Takehiro Miyazaki,Hazrat Belal,Shuji Takabayashi,Chihiro Ohba,Atsushi Takata,Takeshi Mizuguchi,Satoko Miyatake,Noriko Miyake,Atsuo Fukuda,Naomichi Matsumoto,Hirotomo Saitsu

Allergy, asthma & immunology research 13:479-497 PubMed33733641

2021

ATP6V0d2 Suppresses Alveoli Macrophage Alternative Polarization and Allergic Asthma via Degradation of PU.1.

Applications

Unspecified application

Species

Unspecified reactive species

Na Liu,Yuchen Feng,Huicheng Liu,Wenliang Wu,Yuxia Liang,Pingfei Li,Zhengping Wei,Min Wu,Zhao Hui Tang,Junyan Han,Xiang Cheng,Zheng Liu,Arian Laurence,Huabin Li,Guohua Zhen,Xiang Ping Yang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21223 PubMed33715196

2021

The lysosomal membrane protein Sidt2 is a vital regulator of mitochondrial quality control in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Lizhuo Wang,Cui Yu,Wenjun Pei,Mengya Geng,Yao Zhang,Zihui Li,Feiteng Liang,Fengbiao Tan,Hui Du,Jialin Gao

Frontiers in microbiology 11:573844 PubMed33324360

2020

and Escape From the Clearance of Macrophages via Controlling TFEB.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Rao,Tao Xu,Yu Xia,Hongfeng Zhang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ATP6V1A antibody

Immunoprecipitation - Anti-ATP6V1A antibody (AB137574)

Western blot - Anti-ATP6V1A antibody (AB137574)

Western blot - Anti-ATP6V1A antibody (AB137574)

Western blot - Anti-ATP6V1A antibody (AB137574)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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