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AB209823

Anti-ATP6V1A antibody

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(1 Publication)

Rabbit Polyclonal ATP6V1A antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1A aa 50-400.

View Alternative Names

ATP6A1, ATP6V1A1, VPP2, ATP6V1A, V-type proton ATPase catalytic subunit A, V-ATPase subunit A, V-ATPase 69 kDa subunit, Vacuolar ATPase isoform VA68, Vacuolar proton pump subunit alpha

2 Images
IHC - Wholemount - Anti-ATP6V1A antibody (AB209823)
  • IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-ATP6V1A antibody (AB209823)

Immunohistochemical analysis of whole-mount zebrafish embryo (2 days-post-fertilization) labeling ATP6V1A with ab209823 at a 1/100 dilution.

IHC - Wholemount - Anti-ATP6V1A antibody (AB209823)
  • IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-ATP6V1A antibody (AB209823)

Immunohistochemical analysis of paraformaldehyde-fixed whole-mount zebrafish embryo labeling ATP6V1A in epidermal Ionocytes using ab209823 at a 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Zebrafish

Applications

IHC - Wmt

applications

Immunogen

Recombinant Fragment Protein within Human ATP6V1A aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

P38606

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store undiluted
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V1A also known as V-ATPase subunit A is a component of the V1 domain of the vacuolar ATPase (V-ATPase) which is a large enzyme responsible for acidifying intracellular compartments. This protein has a mass of approximately 70 kDa and operates in cellular processes by hydrolyzing ATP to drive protons across membranes. ATP6V1A displays significant expression in several cell types including neurons epithelial cells and osteoclasts. The protein enables V-ATPase functionality important for maintaining the pH balance inside cells and organelles facilitating various physiological processes.
Biological function summary

ATP6V1A is an integral part of the V1 domain of the V-ATPase complex a multi-subunit assembly critical for the acidification of intracellular organelles such as lysosomes endosomes and the Golgi apparatus. This activity supports processes like protein degradation receptor-mediated endocytosis and membrane trafficking. The V-ATPase containing ATP6V1A uses energy from ATP hydrolysis to pump protons which is essential for cellular homeostasis and ion transport.

Pathways

ATP6V1A plays an important role in the endocytosis and autophagy pathways. These pathways depend on the acidification of intracellular compartments enabling processes such as the breakdown and recycling of cellular components. ATP6V1A is related to proteins like V-ATPase subunit c (ATP6V1C) and other subunits that form the V-ATPase enzyme. These protein interactions allow the modulation and execution of cellular transport and hormone regulation pathways.

Mutations or dysregulation of ATP6V1A have been linked to renal tubular acidosis and osteoporosis where improper acidification impacts bone resorption and kidney function. The protein connections include its interaction with other V-ATPase subunits like ATP6V0A3 which can also result in osteopetrosis when defective. Such conditions demonstrate how ATP6V1A's role in acidification processes is important for normal physiological function and how its dysfunction leads to disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 8463241). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed : 32001091). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed : 28296633). May play a role in neurite development and synaptic connectivity (PubMed : 29668857).. (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction.
See full target information ATP6V1A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 18:4877-4885 PubMed30280191

2018

Overexpression of small ubiquitin‑like modifier 2 ameliorates high glucose‑induced reductions in cardiomyocyte proliferation via the transforming growth factor‑β/Smad pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhao,Qile Shen
View all publications
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