AB249327

Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ATP6V1D antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

ATP6M, VATD, ATP6V1D, V-type proton ATPase subunit D, V-ATPase subunit D, V-ATPase 28 kDa accessory protein, Vacuolar proton pump subunit D

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

This data was developed using ab157458, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Paraffin-embedded Human breast tissue labeling ATP6V1D with ab157458 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

This data was developed using ab157458, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling ATP6V1D with ab157458 at 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

This data was developed using ab157458, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling ATP6V1D with ab157458 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

This data was developed using ab157458, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells labeling ATP6V1D with ab157458 at 1/10 dilution (red) or a rabbit IgG negative (green).

Unknown

Immunoprecipitation - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

This data was developed using ab157458, the same antibody clone in a different buffer formulation.

Detection of ATP6V1D by Western Blot of Immunprecipitate. Y79 cell lysate immunoprecipitated using ab157458 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-ATP6V1D antibody [EPR11326(B)] (<a href='/en-us/products/primary-antibodies/atp6v1d-antibody-epr11326b-ab157458'>ab157458</a>) at 1/10000 dilution

All lanes:

Immunoprecipitation pellet from Y79 cell lysate at 10 µg

Predicted band size: 28 kDa

false

Unknown

Western blot - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

This data was developed using ab157458, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ATP6V1D antibody [EPR11326(B)] (<a href='/en-us/products/primary-antibodies/atp6v1d-antibody-epr11326b-ab157458'>ab157458</a>) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Y79 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11326(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IP, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249327 is the carrier-free version of ab157458.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V1D also known as V1D is a subunit of the V1 domain of vacuolar ATPase (V-ATPase). This enzyme is essential for catalyzing ATP hydrolysis and is mitochondrial proton pump playing roles in acidifying various cell compartments. With a molecular weight of approximately 36 kDa ATP6V1D is expressed in many tissues with higher levels noted in the kidney liver and brain. It helps regulate the pH of intracellular compartments important for cellular processes like protein degradation and neurotransmitter uptake.

Biological function summary

ATP6V1D is an integral part of the V-ATPase complex. The V-ATPases have a critical role in the acidification of eukaryotic intracellular compartments which are important for processes such as protein sorting zymogen activation and receptor-mediated endocytosis. This complex operates in various cell types and facilitates the transport of protons across membranes maintaining the acidic environments required for enzyme activity and molecular processing.

Pathways

ATP6V1D participates in key cellular functions such as the Wnt signaling pathway and endocytosis. In the Wnt pathway it contributes to regulating cellular growth and differentiation and interacts with other proteins like ATP6V1A and ATP6V1F to sustain vesicular trafficking and pH homeostasis. These interactions support the movement and function of vesicles affecting the fusion of endosomes and lysosomes essential for cellular responses to external stimuli.

Abnormalities involving ATP6V1D have been linked to neurodegenerative diseases and renal tubular acidosis. Researchers have observed that malfunction of V-ATPases including the ATP6V1D subunit can alter pH regulation in neurons associated with Alzheimer's disease. Furthermore ATP6V1D interactions with proteins such as ATP6V1C1 in the context of impaired proton pumping contribute to metabolic acidosis affecting kidney function and leading to conditions like Type I renal tubular acidosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (PubMed : 21844891).

See full target information ATP6V1D

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

Flow Cytometry (Intracellular) - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

Immunoprecipitation - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

Western blot - Anti-ATP6V1D antibody [EPR11326(B)] - BSA and Azide free (AB249327)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise