Rabbit Polyclonal ATP6V1E1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1E1 aa 1-200.
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- PLoS genetics 17:e10096032021Loss of zebrafish atp6v1e1b, encoding a subunit of vacuolar ATPase, recapitulates human ARCL type 2C syndrome and identifies multiple pathobiological signatures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLore Pottie,Wouter Van Gool,Michiel Vanhooydonck,Franz-Georg Hanisch,Geert Goeminne,Andreja Rajkovic,Paul Coucke,Patrick Sips,Bert CallewaertPubMed 34143769
- Autophagy :1-192020Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy H Ponsford et. al.PubMed 32515674
- Nature microbiology 3:1369-13762018The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomas Doyle et. al.PubMed 30478388
- eLife 7:2018Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZohreh Farsi et. al.PubMed 29652249
- Matrix biology : journal of the International Society for Matrix Biology 52-54:234-2452015V-type ATPase proton pump expression during enamel formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuni Sarkar,Xin Wen,Emil J Simanian,Michael L PainePubMed 26586472
- American journal of physiology. Renal physiology 302:F1121-322011V-ATPase promotes transforming growth factor-β-induced epithelial-mesenchymal transition of rat proximal tubular epithelial cells.Applications:WB, ICC/IFReactive species:RatXueqin Cao,Qiongqiong Yang,Jing Qin,Shili Zhao,Xiaoyan Li,Jinjin Fan,Wenfang Chen,Yi Zhou,Haiping Mao,Xueqing YuPubMed 22129967
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ATP6V1E1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P36543
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:32001091, PubMed:33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091).
Alternative names
ATP6E, ATP6E2, ATP6V1E1, V-type proton ATPase subunit E 1, V-ATPase subunit E 1, V-ATPase 31 kDa subunit, Vacuolar proton pump subunit E 1, p31
Recommended products
Rabbit Polyclonal ATP6V1E1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1E1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ATP6V1E1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P36543
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP6V1E1 also known as V-type proton ATPase subunit E1 is a component of the vacuolar ATPase (V-ATPase) complex which is key in cellular processes like acidification of intracellular organelles. This protein has a mass of approximately 31 kDa and is found extensively in the cytosol. ATP6V1E1 is expressed in various tissues including those in the kidney liver and brain indicating its widespread significance in cellular function.
- Biological function summary
ATP6V1E1 plays a role in energizing the V-ATPase complex which contributes to proton translocation across intracellular membranes. The protein acts within the V1 domain of the V-ATPase a multi-subunit enzyme responsible for acidifying compartments such as endosomes lysosomes and the Golgi apparatus. This acidification is important for protein degradation receptor-mediated endocytosis and neurotransmitter uptake highlighting ATP6V1E1's role in maintaining cellular homeostasis.
- Pathways
ATP6V1E1 is integrated into processes like the autophagy and endocytic pathways. It interacts with other proteins within the V-ATPase complex to regulate organelle pH and support vital functions such as lysosomal degradation. ATP6V1E1's interactions with proteins like ATP6V1A highlights its involvement in these metabolic pathways demonstrating how these components work together to regulate cellular energy and waste management.
- Associated diseases and disorders
ATP6V1E1 has connections to disorders including renal tubular acidosis and osteopetrosis. Mutations in this protein can disrupt the normal acidification process leading to these conditions. Through its role in the V-ATPase complex ATP6V1E1 is functionally linked to other subunits like ATP6V1A. Together they influence the pathophysiology of these diseases by impacting the regulatory mechanisms necessary for stable cellular pH and related metabolic activities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-ATP6V1E1 antibody (ab111733)
12% SDS PAGE
All lanes: Western blot - Anti-ATP6V1E1 antibody (ab111733) at 1/3000 dilution
All lanes: A549 whole cell lysate at 30 µg
Predicted band size: 26 kDa
Western blot - Anti-ATP6V1E1 antibody (ab111733)
12% SDS PAGE
All lanes: Western blot - Anti-ATP6V1E1 antibody (ab111733) at 1/1000 dilution
All lanes: Mouse brain whole cell lysate at 20 µg
Predicted band size: 26 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1E1 antibody (ab111733)
Immunofluorescence analysis of ATP6V1E1 in methanol-fixed HeLa cells, using ab111733 at a 1/200 dilution. Lower image shows the cells counter stained with Hoechst 33342.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-ATP6V1E1 antibody (ab111733)
ATP6V1E1 western blot using anti-ATP6V1E1 antibody ab111733. Publication image and figure legend from Farsi, Z., Gowrisankaran, S., et al., 2018, Elife, PubMed 29652249.
ab111733 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab111733 please see the product overview.
Functional analysis of the vATPase on CCVs.(A) Immunoblots of isolated SVs and CCVs for the clathrin light (LC) and heavy chains (HC), and SV marker proteins synaptophysin (Syph) and VAMP2 (left panel), as well as various Vo and V1 subunits of vATPase (right panel). (B) The ratio of the Vo and V1 as well as synaptophysin and VAMP2 (as SV markers) detected by immunoblot in equal protein amount of CCVs and SVs (C) Normalized levels of Vo and V1 in CCV and SV samples, indicating that Vo:V1 ratio is 1:1 in both preparations. (D) ATPase activity measured in 1.3 µg of isolated SVs and CCVs. (E) Blocking percentage of ATPase activity by NEM (vATPase inhibitor) in 1.3 µg of SVs and CCVs. Error bars in (B–E) represent SD of 3–4 experimental replicates (p<0.01 for D and E, and >0.05 for C).Both Vo and V1 subunits are present on CCVs.Immunoblot for Voa and V1A subunits indicate presence of subunits during the CCV isolation procedure.ATPase activity measurements in isolated SVs.To make sure that the measured ATPase activity is within the standard curve range of the used kit, we tested different amounts of isolated SVs. The measurements of ATPase activity were then performed with 1.3 µg of SVs and CCVs which is in the liner range (inset).
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