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AB251033

Anti-ATP6V1F antibody [EPR15053] - BSA and Azide free

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Rabbit Recombinant Monoclonal ATP6V1F antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.

View Alternative Names

ATP6S14, VATF, ATP6V1F, V-type proton ATPase subunit F, V-ATPase subunit F, V-ATPase 14 kDa subunit, Vacuolar proton pump subunit F

3 Images
Western blot - Anti-ATP6V1F antibody [EPR15053] - BSA and Azide free (AB251033)
  • WB

Supplier Data

Western blot - Anti-ATP6V1F antibody [EPR15053] - BSA and Azide free (AB251033)

This data was developed using ab190789, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ATP6V1F antibody [EPR15053] (<a href='/en-us/products/primary-antibodies/atp6v1f-antibody-epr15053-ab190789'>ab190789</a>) at 1/1000 dilution

All lanes:

Mouse kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 13 kDa,27 kDa,29 kDa,88 kDa

false

Immunoprecipitation - Anti-ATP6V1F antibody [EPR15053] - BSA and Azide free (AB251033)
  • IP

Supplier Data

Immunoprecipitation - Anti-ATP6V1F antibody [EPR15053] - BSA and Azide free (AB251033)

This data was developed using ab190789, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of Human fetal kidney lysate labeling ATP6V1F using ab190789 at 1/100 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-ATP6V1F antibody [EPR15053] (<a href='/en-us/products/primary-antibodies/atp6v1f-antibody-epr15053-ab190789'>ab190789</a>)

Predicted band size: 13 kDa

false

Western blot - Anti-ATP6V1F antibody [EPR15053] - BSA and Azide free (AB251033)
  • WB

Supplier Data

Western blot - Anti-ATP6V1F antibody [EPR15053] - BSA and Azide free (AB251033)

This data was developed using ab190789, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ATP6V1F antibody [EPR15053] (<a href='/en-us/products/primary-antibodies/atp6v1f-antibody-epr15053-ab190789'>ab190789</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15053

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab251033 is the carrier-free version of ab190789.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP6V1F also known as Vacuolar ATPase subunit F is a component of the V-type ATPase (V-ATPase) complex. This protein has a molecular mass of approximately 13 kDa. ATP6V1F is expressed in various tissues with higher levels observed in kidney heart and skeletal muscle tissues. It plays a mechanical role in proton transport across the vacuolar membrane important for acidifying cellular organelles such as endosomes lysosomes and the Golgi apparatus.
Biological function summary

ATP6V1F plays a critical part in cellular processes such as protein degradation ion homeostasis and membrane trafficking. ATP6V1F is a part of the V-ATPase complex which is essential for maintaining the acidification of intracellular compartments. The proton pump activity of V-ATPase involves ATP hydrolysis where ATP6V1F contributes to its regulation and structural stability. It helps in cellular events that require compartmental pH regulation impacting multiple physiological roles.

Pathways

ATP6V1F interacts with processes like endocytosis and lysosome-mediated degradation. The V-ATPase complex which includes ATP6V1F functions in conjunction with proteins such as Rab5 and clathrin during endocytic pathways and is implicated in mTOR signaling. These interactions underline its involvement in both nutrient sensing and cellular metabolism indicating its significance in cellular nutrient dynamics and homeostasis.

ATP6V1F connects to conditions like neurodegenerative diseases and osteopetrosis. Variations in this protein or its dysfunction can lead to improper acidification of lysosomes contributing to neurological disorders through aggregation of misfolded proteins. Additionally its role in osteoclast-mediated bone resorption relates ATP6V1F to osteopetrosis where excessive bone density occurs due to failed bone resorption. The protein complex V-ATPase and its subunits like ATP6V0D1 and ATP6V1B2 often closely correlate with these conditions illustrating a network of related proteins influencing similar pathways and disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed : 33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity).
See full target information ATP6V1F
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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