Rabbit Polyclonal ATP6V1H antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ATP6V1H aa 400-450.
View Alternative Names
CGI-11, ATP6V1H, V-type proton ATPase subunit H, V-ATPase subunit H, Nef-binding protein 1, Protein VMA13 homolog, V-ATPase 50/57 kDa subunits, Vacuolar proton pump subunit H, Vacuolar proton pump subunit SFD, NBP1
- IP
Supplier Data
Immunoprecipitation - Anti-ATP6V1H antibody (AB187706)
Lane 1 : Detection of Human ATP6V1H by Immunoprecipitation from HeLa whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab187706 at 6 μg per reaction.
Lane 2 : Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab187706 at 1 μg/ml.
All lanes:
Immunoprecipitation - Anti-ATP6V1H antibody (ab187706)
Predicted band size: 56 kDa
true
Exposure time: 30s
- WB
Supplier Data
Western blot - Anti-ATP6V1H antibody (AB187706)
All lanes:
Western blot - Anti-ATP6V1H antibody (ab187706) at 1 µg/mL
Lane 1:
HeLa cell lysate at 50 µg
Lane 2:
293T cell lysate at 50 µg
Lane 3:
Jurkat cell lysate at 50 µg
Lane 4:
Mouse cell lysate at 50 µg
Lane 5:
Mouse NIH3T3 cell lysate at 50 µg
Predicted band size: 56 kDa
true
Exposure time: 30s
- WB
CiteAb
Western blot - Anti-ATP6V1H antibody (AB187706)
ATP6V1H western blot using anti-ATP6V1H antibody ab187706. Publication image and figure legend from Farsi, Z., Gowrisankaran, S., et al., 2018, Elife, PubMed 29652249.
ab187706 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab187706 please see the product overview.
Functional analysis of the vATPase on CCVs.(A) Immunoblots of isolated SVs and CCVs for the clathrin light (LC) and heavy chains (HC), and SV marker proteins synaptophysin (Syph) and VAMP2 (left panel), as well as various Vo and V1 subunits of vATPase (right panel). (B) The ratio of the Vo and V1 as well as synaptophysin and VAMP2 (as SV markers) detected by immunoblot in equal protein amount of CCVs and SVs (C) Normalized levels of Vo and V1 in CCV and SV samples, indicating that Vo : V1 ratio is 1 : 1 in both preparations. (D) ATPase activity measured in 1.3 µg of isolated SVs and CCVs. (E) Blocking percentage of ATPase activity by NEM (vATPase inhibitor) in 1.3 µg of SVs and CCVs. Error bars in (B–E) represent SD of 3–4 experimental replicates (p<0.01 for D and E, and >0.05 for C).Both Vo and V1 subunits are present on CCVs.Immunoblot for Voa and V1A subunits indicate presence of subunits during the CCV isolation procedure.ATPase activity measurements in isolated SVs.To make sure that the measured ATPase activity is within the standard curve range of the used kit, we tested different amounts of isolated SVs. The measurements of ATPase activity were then performed with 1.3 µg of SVs and CCVs which is in the liner range (inset).
false
Reactivity data
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATP6V1H functions within the V-ATPase complex which is essential for acidifying intracellular organelles such as lysosomes and endosomes. This subunit contributes to energy-dependent proton transport affecting pH regulation within cells and intracellular processes like protein degradation and receptor-mediated endocytosis. It often cooperates with other subunits within the V-ATPase complex to maintain proper cell function and homeostasis.
Pathways
ATP6V1H participates in critical processes such as endocytosis and protein sorting pathways. These pathways influence cellular environment and nutrient sensing. The ATP6V1H subunit interacts with proteins like ATP6V1A and ATP6V0C which are other components of the V-ATPase complex. Together they form a complex vital for membrane-mediated transport and cellular signaling pathways.
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Publications (3)
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Frontiers in cell and developmental biology 9:743124 PubMed35252216
2022
Applications
Unspecified application
Species
Unspecified reactive species
eLife 8: PubMed31793879
2019
Applications
Unspecified application
Species
Unspecified reactive species
eLife 7: PubMed29652249
2018
Applications
Unspecified application
Species
Unspecified reactive species
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