JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB133731

Anti-ATP7b antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal ATP7B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ATP7B.

View Alternative Names

PWD, WC1, WND, ATP7B, Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP7b antibody (AB133731)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP7b antibody (AB133731)

Immunohistochemical analysis of formalin-fixed, paraffin embedded Human lung (respiratory epithelium) tissue labelling ATP7b with ab133731 at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody (AB133731)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody (AB133731)

Immunofluorescence analysis of HeLa cells, labelling ATP7b with ab133731 at a 1/100 dilution (left image), or immunizing peptide (right image).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ATP7B. The exact immunogen used to generate this antibody is proprietary information.

P35670

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP7B also known as Wilson disease protein is a copper-transporting ATPase. This protein has a mass of approximately 146 kDa. It is mainly expressed in the liver kidney placenta and brain. ATP7B functions mechanically by transporting copper across different cellular compartments. It utilizes ATP hydrolysis to pump copper ions helping the body regulate copper homeostasis. This transport activity is located predominantly in the trans-Golgi network where ATP7B assists in incorporating copper into ceruloplasmin a critical copper-carrying blood plasma protein.
Biological function summary

ATP7B plays a significant role in maintaining copper balance within the body. It associates with intracellular vesicles and through its catalytic activity influences various cellular metabolic processes. Although ATP7B does not typically form large complexes its interaction with other proteins and cellular organelles contributes to copper ion binding and transfer. Proper ATP7B function ensures that copper is channeled effectively to places where it is required for enzymatic activity or is expelled from cells to prevent accumulation.

Pathways

ATP7B is a central figure in the copper transport and homeostasis pathway. Its role is directly connected to the biosynthesis of copper-dependent enzymes. The protein impacts the pathway involving ceruloplasmin biosynthesis by regulating copper ion incorporation. ATP7A another copper-transporting ATPase shares similar pathway responsibilities but in different tissues showing divergence in their specific biological roles. Together they ensure whole-body copper balance.

ATP7B mutations or dysfunction is strongly implicated in Wilson's disease a genetic disorder characterized by excessive copper accumulation. This can result in hepatic neurological and psychiatric symptoms due to copper buildup. The protein's relationship to Wilson's disease suggests its critical role in preventing copper toxicity. There is also evidence linking ATP7B with Menkes disease but ATP7A plays a more direct role in Menkes highlighting the distinct responsibilities these ATPases have in different tissues and conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload.
See full target information ATP7B
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 22:794 PubMed34093750

2021

Effects of extract on lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Huixiang Tian,Yueqin Li,Jie Mei,Lei Cao,Jiye Yin,Zhaoqian Liu,Juan Chen,Xiangping Li

Journal of enzyme inhibition and medicinal chemistry 33:1199-1211 PubMed30132373

2018

Discovery of new chromen-4-one derivatives as telomerase inhibitors through regulating expression of dyskerin.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Quan Wang,Meng Di Yang,Xing Chen,Yang Wang,Liu Zeng Chen,Xiu Cheng,Xin Hua Liu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB240880

Anti-ATP7b antibody [S62-29] - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody [S62-29] - N-terminal (AB240880)

  • 0
  • 0
Primary Antibodies

AB211985

PE Anti-ATP7b antibody [EPR6794]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-ATP7b antibody [EPR6794] (AB211985)

  • 0
  • 0
Primary Antibodies

AB124973

Anti-ATP7b antibody [EPR6794]

KO Validated|RabMAb|Recombinant

Western blot - Anti-ATP7b antibody [EPR6794] (AB124973)

  • 1
  • 1
Primary Antibodies

AB240049

Anti-ATP7b antibody [EPR6793] - BSA and Azide free

RabMAb|Recombinant

OI-RD Scanning - Anti-ATP7b antibody [EPR6793] - BSA and Azide free (AB240049)

  • 0
  • 0
Primary Antibodies

AB240011

Anti-ATP7b antibody [EPR6794] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ATP7b antibody [EPR6794] - BSA and Azide free (AB240011)

  • 0
  • 0
Primary Antibodies

AB131208

Anti-ATP7b antibody [EPR6793]

RabMAb|Recombinant

Western blot - Anti-ATP7b antibody [EPR6793] (AB131208)

  • 4
  • 1

Complementary Products

Assay Kits

AB273296

Ceruloplasmin Activity Assay Kit (Colorimetric)

Functional Studies - Ceruloplasmin Activity Assay Kit (Colorimetric) (AB273296)

  • 0
  • 0
Primary Antibodies

AB131400

Anti-ATP7A antibody [L60/4]

Immunocytochemistry/ Immunofluorescence - Anti-ATP7A antibody [L60/4] (AB131400)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com