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AB131208

Anti-ATP7b antibody [EPR6793]

4

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal ATP7B antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

PWD, WC1, WND, ATP7B, Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein

2 Images
Western blot - Anti-ATP7b antibody [EPR6793] (AB131208)
  • WB

Lab

Western blot - Anti-ATP7b antibody [EPR6793] (AB131208)

The molecular weight observed is consistent with what has been described in PMID : 26438057 and 18673401

All lanes:

Western blot - Anti-ATP7b antibody [EPR6793] (ab131208) at 1/1000 dilution

All lanes:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 157 kDa

Observed band size: 165 kDa

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OI-RD Scanning - Anti-ATP7b antibody [EPR6793] (AB131208)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ATP7b antibody [EPR6793] (AB131208)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-ATP7b antibody [EPR6793] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6793

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP7B also known as Wilson disease protein is a copper-transporting ATPase. This protein has a mass of approximately 146 kDa. It is mainly expressed in the liver kidney placenta and brain. ATP7B functions mechanically by transporting copper across different cellular compartments. It utilizes ATP hydrolysis to pump copper ions helping the body regulate copper homeostasis. This transport activity is located predominantly in the trans-Golgi network where ATP7B assists in incorporating copper into ceruloplasmin a critical copper-carrying blood plasma protein.
Biological function summary

ATP7B plays a significant role in maintaining copper balance within the body. It associates with intracellular vesicles and through its catalytic activity influences various cellular metabolic processes. Although ATP7B does not typically form large complexes its interaction with other proteins and cellular organelles contributes to copper ion binding and transfer. Proper ATP7B function ensures that copper is channeled effectively to places where it is required for enzymatic activity or is expelled from cells to prevent accumulation.

Pathways

ATP7B is a central figure in the copper transport and homeostasis pathway. Its role is directly connected to the biosynthesis of copper-dependent enzymes. The protein impacts the pathway involving ceruloplasmin biosynthesis by regulating copper ion incorporation. ATP7A another copper-transporting ATPase shares similar pathway responsibilities but in different tissues showing divergence in their specific biological roles. Together they ensure whole-body copper balance.

ATP7B mutations or dysfunction is strongly implicated in Wilson's disease a genetic disorder characterized by excessive copper accumulation. This can result in hepatic neurological and psychiatric symptoms due to copper buildup. The protein's relationship to Wilson's disease suggests its critical role in preventing copper toxicity. There is also evidence linking ATP7B with Menkes disease but ATP7A plays a more direct role in Menkes highlighting the distinct responsibilities these ATPases have in different tissues and conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload.
See full target information ATP7B
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40806783

2025

CFAP300 Loss-of-Function Mutations with Primary Ciliary Dyskinesia: Evidence from Ex Vivo and ALI Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Anna G Demchenko,Tatiana A Kyian,Elena I Kondratyeva,Elizaveta E Bragina,Oksana P Ryzhkova,Roman V Veiko,Aleksandra G Nazarova,Vyacheslav B Chernykh,Svetlana A Smirnikhina,Sergey I Kutsev

MedComm 6:e70312 PubMed40761482

2025

Plumbagin Triggers Cuproptosis in Hepatocellular Carcinoma (HCC) via the DNA-Methyltransferase 1 (DNMT1)/microRNA-302a-3p (miR-302a-3p)/ATPase Copper Transporting Beta (ATP7B) Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chuyu Wang,Hao Wang,Chong Wang,Tongtong Tian,Anli Jin,Yu Liu,Ran Huo,Te Liu,Baishen Pan,Wei Guo,Wenjing Yang,Beili Wang

Molecular oncology 15:3527-3544 PubMed34390123

2021

Elesclomol induces copper-dependent ferroptosis in colorectal cancer cells via degradation of ATP7A.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Gao,Zhao Huang,Jiufei Duan,Edouard C Nice,Jie Lin,Canhua Huang

Neurobiology of disease 149:105228 PubMed33359139

2020

The M1311V variant of ATP7A is associated with impaired trafficking and copper homeostasis in models of motor neuron disease.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine Bakkar,Alexander Starr,Benjamin E Rabichow,Ileana Lorenzini,Zachary T McEachin,Robert Kraft,Matthew Chaung,Sam Macklin-Isquierdo,Taylor Wingfield,Briggs Carhart,Nathan Zahler,Wen-Hsuan Chang,Gary J Bassell,Alexandre Betourne,Nicholas Boulis,Samuel V Alworth,Justin K Ichida,Paul R August,Daniela C Zarnescu,Rita Sattler,Robert Bowser

NPJ genomic medicine 5:16 PubMed32284880

2020

variant c.1934T > G p.Met645Arg causes Wilson disease by promoting exon 6 skipping.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Merico,Carl Spickett,Matthew O'Hara,Boyko Kakaradov,Amit G Deshwar,Phil Fradkin,Shreshth Gandhi,Jiexin Gao,Solomon Grant,Ken Kron,Frank W Schmitges,Zvi Shalev,Mark Sun,Marta Verby,Matthew Cahill,James J Dowling,Johan Fransson,Erno Wienholds,Brendan J Frey
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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