AB240880

Anti-ATP7b antibody [S62-29] - N-terminal

Name: Anti-ATP7b antibody [S62-29] - N-terminal (ab240880) | Abcam
Brand: Abcam Limited
SKU: AB240880
Price: 520 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal ATP7B antibody. N-terminal. Suitable for ICC/IF and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATP7B aa 1-50.

View Alternative Names

PWD, WC1, WND, ATP7B, Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody [S62-29] - N-terminal (AB240880)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody [S62-29] - N-terminal (AB240880)

4% Formaldehyde-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells stained for ATP7b (Green) using ab240880 at 1/100 dilution for 60 minutes at RT. The secondary antibody used was a Goat Anti-Mouse ATTO 488 at 1 : 200 for 60 min at RT. Phalloidin Texas Red F-Actin stain was used as a counterstain (Red) at 1/1000 dilution for 60 minutes at RT. The nuclear counterstain was DAPI (Blue) at 1/5000 dilution for 5 minutes at RT.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

S62-29

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human ATP7B aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P35670

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP7B also known as Wilson disease protein is a copper-transporting ATPase. This protein has a mass of approximately 146 kDa. It is mainly expressed in the liver kidney placenta and brain. ATP7B functions mechanically by transporting copper across different cellular compartments. It utilizes ATP hydrolysis to pump copper ions helping the body regulate copper homeostasis. This transport activity is located predominantly in the trans-Golgi network where ATP7B assists in incorporating copper into ceruloplasmin a critical copper-carrying blood plasma protein.

Biological function summary

ATP7B plays a significant role in maintaining copper balance within the body. It associates with intracellular vesicles and through its catalytic activity influences various cellular metabolic processes. Although ATP7B does not typically form large complexes its interaction with other proteins and cellular organelles contributes to copper ion binding and transfer. Proper ATP7B function ensures that copper is channeled effectively to places where it is required for enzymatic activity or is expelled from cells to prevent accumulation.

Pathways

ATP7B is a central figure in the copper transport and homeostasis pathway. Its role is directly connected to the biosynthesis of copper-dependent enzymes. The protein impacts the pathway involving ceruloplasmin biosynthesis by regulating copper ion incorporation. ATP7A another copper-transporting ATPase shares similar pathway responsibilities but in different tissues showing divergence in their specific biological roles. Together they ensure whole-body copper balance.

ATP7B mutations or dysfunction is strongly implicated in Wilson's disease a genetic disorder characterized by excessive copper accumulation. This can result in hepatic neurological and psychiatric symptoms due to copper buildup. The protein's relationship to Wilson's disease suggests its critical role in preventing copper toxicity. There is also evidence linking ATP7B with Menkes disease but ATP7A plays a more direct role in Menkes highlighting the distinct responsibilities these ATPases have in different tissues and conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload.

See full target information ATP7B

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Kaohsiung journal of medical sciences 39:916-926 PubMed37338034

2023

Suppression of cAMP/PKA/CREB signaling ameliorates retinal injury in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Ling Fang,Qin Zhang,Wen-Wen Xue,Jin-Hua Tao,Hai-Dong Zou,Qiu-Rong Lin,Yu-Lan Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP7b antibody [S62-29] - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-ATP7b antibody [S62-29] - N-terminal (AB240880)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Proteins & Peptides

Product promise